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- W2894956255 abstract "Abstract The NLRP3 inflammasome is a well-studied target for the treatment of multiple inflammatory diseases, but how to promote the current therapeutics remains a large challenge. CRISPR/Cas9, as a gene editing tool, allows for direct ablation of NLRP3 at the genomic level. In this study, we screen an optimized cationic lipid-assisted nanoparticle (CLAN) to deliver Cas9 mRNA (mCas9) and guide RNA (gRNA) into macrophages. By using CLAN encapsulating mCas9 and gRNA-targeting NLRP3 (gNLRP3) (CLAN mCas9/gNLRP3 ), we disrupt NLRP3 of macrophages, inhibiting the activation of the NLRP3 inflammasome in response to diverse stimuli. After intravenous injection, CLAN mCas9/gNLRP3 mitigates acute inflammation of LPS-induced septic shock and monosodium urate crystal (MSU)-induced peritonitis. In addition, CLAN mCas9/gNLRP3 treatment improves insulin sensitivity and reduces adipose inflammation of high-fat-diet (HFD)-induced type 2 diabetes (T2D). Thus, our study provides a promising strategy for treating NLRP3-dependent inflammatory diseases and provides a carrier for delivering CRISPR/Cas9 into macrophages." @default.
- W2894956255 created "2018-10-12" @default.
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- W2894956255 date "2018-10-05" @default.
- W2894956255 modified "2023-10-16" @default.
- W2894956255 title "Targeting of NLRP3 inflammasome with gene editing for the amelioration of inflammatory diseases" @default.
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- W2894956255 doi "https://doi.org/10.1038/s41467-018-06522-5" @default.
- W2894956255 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6173702" @default.
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- W2894956255 hasPublicationYear "2018" @default.
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