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- W2894969429 abstract "To explore genome mutation meaningfully, it is in urgent need to develop an automated and inexpensive platform for DNA mutation analysis. Digital microfluidics is a powerful platform for a broad range of applications due to the advantages of high automatization and low reagent consumption. Pyrosequencing enables DNA sequencing based on non-electrophoresis bioluminescence, which is suitable for rapid and sensitive analysis of short sequences. Herein, we describe a palmtop sequencing platform for automatic, real-time and portable analysis of DNA mutations, which is based on the pyrosequencing principle and implemented by digital microfluidics. The portable system can sequence a DNA template with up to 53 bp with 100% accuracy within 2 h. Mutation in the KRAS gene can be detected within 30 min with a LOD as low as 5% mutant level. Portable and accurate gender identification was further demonstrated by sequencing a short amelogenin fragment. With the advantages of portability, ease of use, high accuracy, and low cost, the palmtop sequencing platform shows great potential for portable genetic testing in a variety of circumstances." @default.
- W2894969429 created "2018-10-12" @default.
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- W2894969429 date "2019-02-01" @default.
- W2894969429 modified "2023-10-16" @default.
- W2894969429 title "Rapid, real-time chemiluminescent detection of DNA mutation based on digital microfluidics and pyrosequencing" @default.
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- W2894969429 doi "https://doi.org/10.1016/j.bios.2018.09.092" @default.
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