FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2894979603> ?p ?o ?g. }

• W2894979603 endingPage "6678" @default.
• W2894979603 startingPage "6669" @default.
• W2894979603 abstract "The ability of histidine to participate in a wide range of stabilizing polar interactions preferentially populates this residue in functionally important sites of proteins. Histidine possesses an amphiphilic and electrostatic nature that is essential for amino acids residing at membrane interfaces. However, the frequency of occurrence of histidine at membrane interfaces, particularly transmembrane β-barrels, is lower than those of other aromatic residues. Here, we carry out comprehensive energetic measurements using equilibrium folding of the outer membrane enzyme PagP to address the contribution of a C-terminal interface histidine to barrel stability. We show that placing histidine at the C-terminus universally destabilizes PagP by 4.0-8.0 kcal mol-1 irrespective of the neighboring residue. Spectroscopic and electrophoretic measurements indicate that the altered stability may arise from a loss of barrel compaction. Isoleucine, methionine, and valine salvage this destabilization marginally (in addition to tyrosine, which shows an exceptionally high folding free energy value), when placed at the penultimate position, at the expense of an altered folding pathway. Double-mutant cycle analysis indicates that the coupling energy between the terminal and penultimate residues in PagP-X160H161 increases when the level of intrinsic destabilization by the terminal H161 is high. Our observations that neighboring residues cannot salvage the energetic destabilization of histidine may explain why histidine is less abundant at membrane interfaces." @default.
• W2894979603 created "2018-10-12" @default.
• W2894979603 creator A5009443579 @default.
• W2894979603 creator A5022686532 @default.
• W2894979603 creator A5027353929 @default.
• W2894979603 creator A5031226525 @default.
• W2894979603 creator A5048940255 @default.
• W2894979603 date "2018-10-04" @default.
• W2894979603 modified "2023-10-17" @default.
• W2894979603 title "Salvaging the Thermodynamic Destabilization of Interface Histidine in Transmembrane β-Barrels" @default.
• W2894979603 cites W1553900867 @default.
• W2894979603 cites W1968326351 @default.
• W2894979603 cites W1971078428 @default.
• W2894979603 cites W1972442457 @default.
• W2894979603 cites W1974061371 @default.
• W2894979603 cites W1985204165 @default.
• W2894979603 cites W1990122271 @default.
• W2894979603 cites W1991825531 @default.
• W2894979603 cites W1994190865 @default.
• W2894979603 cites W1996478334 @default.
• W2894979603 cites W2003434089 @default.
• W2894979603 cites W2004181413 @default.
• W2894979603 cites W2004547923 @default.
• W2894979603 cites W2008251757 @default.
• W2894979603 cites W2010499374 @default.
• W2894979603 cites W2014101652 @default.
• W2894979603 cites W2017076970 @default.
• W2894979603 cites W2018131055 @default.
• W2894979603 cites W2019215286 @default.
• W2894979603 cites W2022077899 @default.
• W2894979603 cites W2025859600 @default.
• W2894979603 cites W2026270597 @default.
• W2894979603 cites W2026593599 @default.
• W2894979603 cites W2026907061 @default.
• W2894979603 cites W2030353309 @default.
• W2894979603 cites W2032240634 @default.
• W2894979603 cites W2046763527 @default.
• W2894979603 cites W2047376279 @default.
• W2894979603 cites W2049562138 @default.
• W2894979603 cites W2053754839 @default.
• W2894979603 cites W2068265376 @default.
• W2894979603 cites W2076541507 @default.
• W2894979603 cites W2078619116 @default.
• W2894979603 cites W2081288871 @default.
• W2894979603 cites W2081336227 @default.
• W2894979603 cites W2092661111 @default.
• W2894979603 cites W2104534420 @default.
• W2894979603 cites W2110089370 @default.
• W2894979603 cites W2121372091 @default.
• W2894979603 cites W2127922494 @default.
• W2894979603 cites W2138836809 @default.
• W2894979603 cites W2142267788 @default.
• W2894979603 cites W2145602919 @default.
• W2894979603 cites W2155085669 @default.
• W2894979603 cites W2156961003 @default.
• W2894979603 cites W2165870614 @default.
• W2894979603 cites W2192218325 @default.
• W2894979603 cites W2283046745 @default.
• W2894979603 cites W2306692283 @default.
• W2894979603 cites W2461988432 @default.
• W2894979603 cites W2476261036 @default.
• W2894979603 cites W2496298880 @default.
• W2894979603 cites W2622962302 @default.
• W2894979603 cites W2741125573 @default.
• W2894979603 cites W2744467258 @default.
• W2894979603 cites W2762193974 @default.
• W2894979603 cites W2783213712 @default.
• W2894979603 doi "https://doi.org/10.1021/acs.biochem.8b00805" @default.
• W2894979603 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6284319" @default.
• W2894979603 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30284812" @default.
• W2894979603 hasPublicationYear "2018" @default.
• W2894979603 type Work @default.
• W2894979603 sameAs 2894979603 @default.
• W2894979603 citedByCount "8" @default.
• W2894979603 countsByYear W28949796032019 @default.
• W2894979603 countsByYear W28949796032020 @default.
• W2894979603 countsByYear W28949796032022 @default.
• W2894979603 countsByYear W28949796032023 @default.
• W2894979603 crossrefType "journal-article" @default.
• W2894979603 hasAuthorship W2894979603A5009443579 @default.
• W2894979603 hasAuthorship W2894979603A5022686532 @default.
• W2894979603 hasAuthorship W2894979603A5027353929 @default.
• W2894979603 hasAuthorship W2894979603A5031226525 @default.
• W2894979603 hasAuthorship W2894979603A5048940255 @default.
• W2894979603 hasBestOaLocation W28949796031 @default.
• W2894979603 hasConcept C118892022 @default.
• W2894979603 hasConcept C119599485 @default.
• W2894979603 hasConcept C12554922 @default.
• W2894979603 hasConcept C127413603 @default.
• W2894979603 hasConcept C170493617 @default.
• W2894979603 hasConcept C185592680 @default.
• W2894979603 hasConcept C204328495 @default.
• W2894979603 hasConcept C24530287 @default.
• W2894979603 hasConcept C2776545253 @default.
• W2894979603 hasConcept C2776580952 @default.
• W2894979603 hasConcept C2777305461 @default.
• W2894979603 hasConcept C2778460671 @default.
• W2894979603 hasConcept C41625074 @default.

• next