FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895028872> ?p ?o ?g. }

• W2895028872 abstract "An HIV cure has not yet been achieved because latent viral reservoirs persist, particularly in resting CD4+ T lymphocytes. In vitro, it is difficult to infect resting CD4+ T cells with HIV-1, but infections readily occur in vivo. Endothelial cells (EC) line the lymphatic vessels in the lymphoid tissues and regularly interact with resting CD4+ T cells in vivo. Others and we have shown that EC promoted productive and latent HIV infection of resting CD4+ T cells. However, the EC used in previous studies were from human umbilical cords (HUVEC), which are macrovascular; whereas EC residing in the lymphoid tissues are microvascular.In this study, we investigated the effects of microvascular EC stimulation of resting CD4+ T cells in establishing viral infection and latency. Human resting and activated CD4+ T cells were cultured alone or with endothelial cells and infected with a pseudotyped virus. Infection levels, indicated by green fluorescent protein expression, were measured with flow cytometry and data was analyzed using Flowing Software and Excel.We confirmed that EC from lymphatic tissue (LEC) were able to promote HIV infection and latency formation in resting CD4+ T cells while keeping them in resting phenotype, and that IL-6 was involved in LEC stimulation of CD4+ T cells. However, there are some differences between stimulation by LEC and HUVEC. Unlike HUVEC stimulation, we demonstrated that LEC stimulation of resting memory T cells does not depend on major histocompatibility complex class II (MHC II) interactions with T cell receptors (TCR) and that CD2-CD58 interactions were not involved in LEC stimulation of resting T cells. LEC also secreted lower levels of IL-6 than HUVEC. We also found that LEC stimulation increases HIV infection rates in activated CD4+ T cells.While differences in T cell stimulation between lymphatic EC and HUVEC were observed, we confirmed that similar to macrovascular EC stimulation, microvascular EC stimulation promotes direct HIV infection and latency formation in resting CD4+ T cells without T cell activation. LEC stimulation also increased infection rates in activated CD4+ T cells. Additionally, the present study established a physiologically more relevant model of EC interactions with resting CD4+ T cells and further highlighted the importance of investigating the roles of EC in HIV infection and latency in both resting and activated CD4+ T cells." @default.
• W2895028872 created "2018-10-12" @default.
• W2895028872 creator A5005899718 @default.
• W2895028872 creator A5025106192 @default.
• W2895028872 creator A5043144487 @default.
• W2895028872 creator A5051356582 @default.
• W2895028872 creator A5053132847 @default.
• W2895028872 creator A5076596725 @default.
• W2895028872 creator A5082312925 @default.
• W2895028872 creator A5085116538 @default.
• W2895028872 date "2018-10-03" @default.
• W2895028872 modified "2023-09-27" @default.
• W2895028872 title "Lymphatic endothelial cells promote productive and latent HIV infection in resting CD4+ T cells" @default.
• W2895028872 cites W1546915777 @default.
• W2895028872 cites W1595689392 @default.
• W2895028872 cites W1869438582 @default.
• W2895028872 cites W1909719355 @default.
• W2895028872 cites W1982206175 @default.
• W2895028872 cites W1985383725 @default.
• W2895028872 cites W1991037689 @default.
• W2895028872 cites W1996321192 @default.
• W2895028872 cites W1999396723 @default.
• W2895028872 cites W2014490414 @default.
• W2895028872 cites W2018312298 @default.
• W2895028872 cites W2019508559 @default.
• W2895028872 cites W2064685550 @default.
• W2895028872 cites W2075311313 @default.
• W2895028872 cites W2080653140 @default.
• W2895028872 cites W2083283696 @default.
• W2895028872 cites W2095222585 @default.
• W2895028872 cites W2095592359 @default.
• W2895028872 cites W2109194095 @default.
• W2895028872 cites W2117013048 @default.
• W2895028872 cites W2148951908 @default.
• W2895028872 cites W2152129968 @default.
• W2895028872 cites W2152204052 @default.
• W2895028872 cites W2153499980 @default.
• W2895028872 cites W2155462089 @default.
• W2895028872 cites W2158676450 @default.
• W2895028872 cites W2160621761 @default.
• W2895028872 cites W2216066435 @default.
• W2895028872 cites W2225775312 @default.
• W2895028872 cites W2413164300 @default.
• W2895028872 cites W2512749206 @default.
• W2895028872 cites W2567822565 @default.
• W2895028872 cites W2589110511 @default.
• W2895028872 cites W2607524928 @default.
• W2895028872 cites W2736205158 @default.
• W2895028872 cites W2766543637 @default.
• W2895028872 cites W2782696110 @default.
• W2895028872 cites W2804663340 @default.
• W2895028872 cites W49920453 @default.
• W2895028872 cites W628485609 @default.
• W2895028872 doi "https://doi.org/10.1186/s12985-018-1068-6" @default.
• W2895028872 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6169068" @default.
• W2895028872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30285810" @default.
• W2895028872 hasPublicationYear "2018" @default.
• W2895028872 type Work @default.
• W2895028872 sameAs 2895028872 @default.
• W2895028872 citedByCount "7" @default.
• W2895028872 countsByYear W28950288722019 @default.
• W2895028872 countsByYear W28950288722020 @default.
• W2895028872 countsByYear W28950288722022 @default.
• W2895028872 countsByYear W28950288722023 @default.
• W2895028872 crossrefType "journal-article" @default.
• W2895028872 hasAuthorship W2895028872A5005899718 @default.
• W2895028872 hasAuthorship W2895028872A5025106192 @default.
• W2895028872 hasAuthorship W2895028872A5043144487 @default.
• W2895028872 hasAuthorship W2895028872A5051356582 @default.
• W2895028872 hasAuthorship W2895028872A5053132847 @default.
• W2895028872 hasAuthorship W2895028872A5076596725 @default.
• W2895028872 hasAuthorship W2895028872A5082312925 @default.
• W2895028872 hasAuthorship W2895028872A5085116538 @default.
• W2895028872 hasBestOaLocation W28950288721 @default.
• W2895028872 hasConcept C134018914 @default.
• W2895028872 hasConcept C181152851 @default.
• W2895028872 hasConcept C203014093 @default.
• W2895028872 hasConcept C207936829 @default.
• W2895028872 hasConcept C24998067 @default.
• W2895028872 hasConcept C2776090121 @default.
• W2895028872 hasConcept C2910366181 @default.
• W2895028872 hasConcept C553184892 @default.
• W2895028872 hasConcept C86803240 @default.
• W2895028872 hasConcept C8891405 @default.
• W2895028872 hasConcept C95444343 @default.
• W2895028872 hasConceptScore W2895028872C134018914 @default.
• W2895028872 hasConceptScore W2895028872C181152851 @default.
• W2895028872 hasConceptScore W2895028872C203014093 @default.
• W2895028872 hasConceptScore W2895028872C207936829 @default.
• W2895028872 hasConceptScore W2895028872C24998067 @default.
• W2895028872 hasConceptScore W2895028872C2776090121 @default.
• W2895028872 hasConceptScore W2895028872C2910366181 @default.
• W2895028872 hasConceptScore W2895028872C553184892 @default.
• W2895028872 hasConceptScore W2895028872C86803240 @default.
• W2895028872 hasConceptScore W2895028872C8891405 @default.
• W2895028872 hasConceptScore W2895028872C95444343 @default.
• W2895028872 hasFunder F4320337355 @default.
• W2895028872 hasIssue "1" @default.
• W2895028872 hasLocation W28950288721 @default.
• W2895028872 hasLocation W28950288722 @default.

• next