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- W2895044634 abstract "Preparation of poly[ethylene glycol dimethacrylate (EGDMA)-glycidyl methacrylate (GMA)] cryogel and its usability for amyloglucosidase adsorption were investigated. Cryogels were prepared by cryocopolymerization technique and then functionalized by lectin concanavalin A (Con A). The synthesized cryogel was characterized by FTIR, SEM and EDX analysis and then used for adsorption of amyloglucosidase in a continuous flow system. The maximal amyloglucosidase adsorption efficiency of Con A attached cryogel was found to be as 30.50 mg/g cryogel. Con A modified cryogels were used repeatedly for 30 times without any significant decrease at the amyloglucosidase adsorption capacity. SDS-PAGE and activity studies confirmed that the desorbed amyloglucosidase was active and retained 90% of its initial activity after the adsorption/desorption processes." @default.
- W2895044634 created "2018-10-12" @default.
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- W2895044634 date "2018-06-13" @default.
- W2895044634 modified "2023-09-26" @default.
- W2895044634 title "Lectin attached affinity cryogels for amyloglucosidase adsorption" @default.
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- W2895044634 doi "https://doi.org/10.1080/07328303.2018.1487972" @default.
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