Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895051635> ?p ?o ?g. }
- W2895051635 endingPage "98" @default.
- W2895051635 startingPage "92" @default.
- W2895051635 abstract "Perfluorooctanoic acid (PFOA) is a member of the perfluoroalkyl acid family of compounds. Due to the presence of strong carbon-fluorine bonds, it is practically nonbiodegradable and highly persistent in the environment. PFOA has been detected in the follicular fluid of women, and positively associated with reduced fecundability and infertility. However, there are no reports concerning the experimental evaluation of PFOA on oocyte toxicity in mammals. The aim of the present study was to determine if PFOA is able to induce oxidative stress in fetal ovaries and cause apoptosis in oocytes in vitro. In addition, since inhibition of the gap junction intercellular communication (GJIC) by PFOA has been demonstrated in liver cells in vivo and in vitro, the effect of PFOA on the GJIC between the oocyte and its supportive cumulus cells was studied. Results show that PFOA induced oocyte apoptosis and necrosis in vitro (medium lethal concentration, LC50 = 112.8 μM), as evaluated with Annexin-V-Alexa 508 in combination with BOBO-1 staining. Reactive oxygen species (ROS) levels, as assessed by DCFH-DA, increased significantly in fetal ovaries exposed to ¼ LC50 (28.2 μM, a noncytotoxic and relevant occupational exposure concentration) and LC50 PFOA ex vivo. This perfluorinated compound also caused the blockage of GJIC in cumulus cells-oocyte complexes (COCs) obtained from female mice exposed in vivo, as evaluated by calcein transfer from cumulus cells to the oocyte. The ability of PFOA of disrupting the GJIC in COCs, generating ROS in the fetal ovary and causing apoptosis and necrosis in mammal's oocytes, might account for the reported association between increasing maternal plasma concentrations of PFOA with reduced fertility in women." @default.
- W2895051635 created "2018-10-12" @default.
- W2895051635 creator A5001860913 @default.
- W2895051635 creator A5009873879 @default.
- W2895051635 creator A5010274197 @default.
- W2895051635 creator A5024912074 @default.
- W2895051635 creator A5025193137 @default.
- W2895051635 creator A5027804081 @default.
- W2895051635 creator A5031226242 @default.
- W2895051635 creator A5046878859 @default.
- W2895051635 creator A5052685177 @default.
- W2895051635 creator A5062493864 @default.
- W2895051635 creator A5070587488 @default.
- W2895051635 creator A5076431275 @default.
- W2895051635 creator A5076798593 @default.
- W2895051635 creator A5088047168 @default.
- W2895051635 creator A5088318943 @default.
- W2895051635 date "2018-10-02" @default.
- W2895051635 modified "2023-10-16" @default.
- W2895051635 title "Perfluorooctanoic acid disrupts gap junction intercellular communication and induces reactive oxygen species formation and apoptosis in mouse ovaries" @default.
- W2895051635 cites W1550247880 @default.
- W2895051635 cites W1599628769 @default.
- W2895051635 cites W1951187588 @default.
- W2895051635 cites W1975902105 @default.
- W2895051635 cites W1981868507 @default.
- W2895051635 cites W1988117548 @default.
- W2895051635 cites W1993650845 @default.
- W2895051635 cites W2000335232 @default.
- W2895051635 cites W2001372478 @default.
- W2895051635 cites W2018190812 @default.
- W2895051635 cites W2025545200 @default.
- W2895051635 cites W2035259215 @default.
- W2895051635 cites W2054420964 @default.
- W2895051635 cites W2055576275 @default.
- W2895051635 cites W2056990725 @default.
- W2895051635 cites W2062240397 @default.
- W2895051635 cites W2062265328 @default.
- W2895051635 cites W2089669600 @default.
- W2895051635 cites W2106224369 @default.
- W2895051635 cites W2117859857 @default.
- W2895051635 cites W2122421156 @default.
- W2895051635 cites W2128473487 @default.
- W2895051635 cites W2134791901 @default.
- W2895051635 cites W2143474573 @default.
- W2895051635 cites W2150124281 @default.
- W2895051635 cites W2155724120 @default.
- W2895051635 cites W2179490415 @default.
- W2895051635 cites W2406685744 @default.
- W2895051635 cites W2410531503 @default.
- W2895051635 cites W2511523878 @default.
- W2895051635 cites W2549117997 @default.
- W2895051635 cites W2590486243 @default.
- W2895051635 cites W2605583035 @default.
- W2895051635 cites W2621491632 @default.
- W2895051635 cites W2751306307 @default.
- W2895051635 cites W2762482504 @default.
- W2895051635 cites W2884311292 @default.
- W2895051635 cites W4211262799 @default.
- W2895051635 doi "https://doi.org/10.1002/tox.22661" @default.
- W2895051635 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30277307" @default.
- W2895051635 hasPublicationYear "2018" @default.
- W2895051635 type Work @default.
- W2895051635 sameAs 2895051635 @default.
- W2895051635 citedByCount "44" @default.
- W2895051635 countsByYear W28950516352020 @default.
- W2895051635 countsByYear W28950516352021 @default.
- W2895051635 countsByYear W28950516352022 @default.
- W2895051635 countsByYear W28950516352023 @default.
- W2895051635 crossrefType "journal-article" @default.
- W2895051635 hasAuthorship W2895051635A5001860913 @default.
- W2895051635 hasAuthorship W2895051635A5009873879 @default.
- W2895051635 hasAuthorship W2895051635A5010274197 @default.
- W2895051635 hasAuthorship W2895051635A5024912074 @default.
- W2895051635 hasAuthorship W2895051635A5025193137 @default.
- W2895051635 hasAuthorship W2895051635A5027804081 @default.
- W2895051635 hasAuthorship W2895051635A5031226242 @default.
- W2895051635 hasAuthorship W2895051635A5046878859 @default.
- W2895051635 hasAuthorship W2895051635A5052685177 @default.
- W2895051635 hasAuthorship W2895051635A5062493864 @default.
- W2895051635 hasAuthorship W2895051635A5070587488 @default.
- W2895051635 hasAuthorship W2895051635A5076431275 @default.
- W2895051635 hasAuthorship W2895051635A5076798593 @default.
- W2895051635 hasAuthorship W2895051635A5088047168 @default.
- W2895051635 hasAuthorship W2895051635A5088318943 @default.
- W2895051635 hasBestOaLocation W28950516351 @default.
- W2895051635 hasConcept C150903083 @default.
- W2895051635 hasConcept C16685009 @default.
- W2895051635 hasConcept C185592680 @default.
- W2895051635 hasConcept C190283241 @default.
- W2895051635 hasConcept C196843134 @default.
- W2895051635 hasConcept C207001950 @default.
- W2895051635 hasConcept C2775979320 @default.
- W2895051635 hasConcept C2776151105 @default.
- W2895051635 hasConcept C2776690073 @default.
- W2895051635 hasConcept C48349386 @default.
- W2895051635 hasConcept C55493867 @default.
- W2895051635 hasConcept C71924100 @default.
- W2895051635 hasConcept C86803240 @default.