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- W2895055857 abstract "It is essential to prepare a standard curve for every real-time polymerase chain reaction (PCR) experiment. This protocol is used to construct a standard curve in which the template concentration is unknown. Such a standard curve is suitable for optimization experiments and for performing relative quantification by the standard curve method. To construct a standard curve for absolute quantification, the same principles apply as those presented here, but the concentration of the standards must be determined by an independent method." @default.
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- W2895055857 date "2018-10-01" @default.
- W2895055857 modified "2023-09-26" @default.
- W2895055857 title "Constructing a Standard Curve for Real-Time Polymerase Chain Reaction (PCR) Experiments" @default.
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- W2895055857 doi "https://doi.org/10.1101/pdb.prot095026" @default.
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