Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895072689> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2895072689 abstract "A previously developed RNA polymerase ribozyme uses nucleoside triphosphates (NTPs) to extend a primer 3′-terminus, templated by an RNA template with good fidelity, forming 3′-5′-phosphordiester bonds. Indirect evidence has suggested that the ribozyme's accessory domain binds the NTP with a highly conserved purine-rich loop. To determine the NTP binding site more precisely we evolved the ribozyme for efficient use of 6-thio guanosine triphosphate (6sGTP). 6sGTP never appeared in the evolutionary history of the ribozyme, therefore it was expected that mutations would appear at the NTP binding site, adapting to more efficient binding of 6sGTP. Indeed, the evolution identified three mutations that mediate 200-fold improved incorporation kinetics for 6sGTP. A >50-fold effect resulted from mutation A156U in the purine-rich loop, identifying the NTP binding site. This mutation acted weakly cooperative with two other beneficial mutations, C113U in the P2 stem near the catalytic site, and C79U on the surface of the catalytic domain. The preference pattern of the ribozyme for different NTPs changed when position 156 was mutated, confirming a direct contact between position 156 and the NTP. The results suggest that A156 stabilizes the NTP in the active site by a hydrogen bond to the Hoogsteen face of the NTP." @default.
- W2895072689 created "2018-10-12" @default.
- W2895072689 creator A5015338246 @default.
- W2895072689 creator A5025144497 @default.
- W2895072689 date "2018-10-05" @default.
- W2895072689 modified "2023-09-23" @default.
- W2895072689 title "The NTP binding site of the polymerase ribozyme" @default.
- W2895072689 cites W1633944576 @default.
- W2895072689 cites W1976374524 @default.
- W2895072689 cites W1980461160 @default.
- W2895072689 cites W1988349747 @default.
- W2895072689 cites W1995143868 @default.
- W2895072689 cites W2003825614 @default.
- W2895072689 cites W2017272474 @default.
- W2895072689 cites W2020781311 @default.
- W2895072689 cites W2037238732 @default.
- W2895072689 cites W2046538112 @default.
- W2895072689 cites W2050110866 @default.
- W2895072689 cites W2061091972 @default.
- W2895072689 cites W2075152880 @default.
- W2895072689 cites W2078089564 @default.
- W2895072689 cites W2078362730 @default.
- W2895072689 cites W2082854559 @default.
- W2895072689 cites W2091002791 @default.
- W2895072689 cites W2091610800 @default.
- W2895072689 cites W2095239103 @default.
- W2895072689 cites W2098021908 @default.
- W2895072689 cites W2107057327 @default.
- W2895072689 cites W2111443395 @default.
- W2895072689 cites W2119473941 @default.
- W2895072689 cites W2135636849 @default.
- W2895072689 cites W2152695725 @default.
- W2895072689 cites W2395271787 @default.
- W2895072689 cites W2515842261 @default.
- W2895072689 cites W2808369312 @default.
- W2895072689 cites W3042165321 @default.
- W2895072689 cites W4235033012 @default.
- W2895072689 cites W4238858501 @default.
- W2895072689 doi "https://doi.org/10.1093/nar/gky898" @default.
- W2895072689 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6237761" @default.
- W2895072689 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30289487" @default.
- W2895072689 hasPublicationYear "2018" @default.
- W2895072689 type Work @default.
- W2895072689 sameAs 2895072689 @default.
- W2895072689 citedByCount "3" @default.
- W2895072689 countsByYear W28950726892021 @default.
- W2895072689 countsByYear W28950726892023 @default.
- W2895072689 crossrefType "journal-article" @default.
- W2895072689 hasAuthorship W2895072689A5015338246 @default.
- W2895072689 hasAuthorship W2895072689A5025144497 @default.
- W2895072689 hasBestOaLocation W28950726891 @default.
- W2895072689 hasConcept C104317684 @default.
- W2895072689 hasConcept C107824862 @default.
- W2895072689 hasConcept C12554922 @default.
- W2895072689 hasConcept C153911025 @default.
- W2895072689 hasConcept C181199279 @default.
- W2895072689 hasConcept C2777995097 @default.
- W2895072689 hasConcept C30951146 @default.
- W2895072689 hasConcept C41183919 @default.
- W2895072689 hasConcept C55493867 @default.
- W2895072689 hasConcept C66144319 @default.
- W2895072689 hasConcept C67705224 @default.
- W2895072689 hasConcept C76346623 @default.
- W2895072689 hasConcept C86803240 @default.
- W2895072689 hasConceptScore W2895072689C104317684 @default.
- W2895072689 hasConceptScore W2895072689C107824862 @default.
- W2895072689 hasConceptScore W2895072689C12554922 @default.
- W2895072689 hasConceptScore W2895072689C153911025 @default.
- W2895072689 hasConceptScore W2895072689C181199279 @default.
- W2895072689 hasConceptScore W2895072689C2777995097 @default.
- W2895072689 hasConceptScore W2895072689C30951146 @default.
- W2895072689 hasConceptScore W2895072689C41183919 @default.
- W2895072689 hasConceptScore W2895072689C55493867 @default.
- W2895072689 hasConceptScore W2895072689C66144319 @default.
- W2895072689 hasConceptScore W2895072689C67705224 @default.
- W2895072689 hasConceptScore W2895072689C76346623 @default.
- W2895072689 hasConceptScore W2895072689C86803240 @default.
- W2895072689 hasFunder F4320306101 @default.
- W2895072689 hasLocation W28950726891 @default.
- W2895072689 hasLocation W28950726892 @default.
- W2895072689 hasLocation W28950726893 @default.
- W2895072689 hasLocation W28950726894 @default.
- W2895072689 hasLocation W28950726895 @default.
- W2895072689 hasOpenAccess W2895072689 @default.
- W2895072689 hasPrimaryLocation W28950726891 @default.
- W2895072689 hasRelatedWork W1997953830 @default.
- W2895072689 hasRelatedWork W2041331047 @default.
- W2895072689 hasRelatedWork W2047683652 @default.
- W2895072689 hasRelatedWork W2071127957 @default.
- W2895072689 hasRelatedWork W2105314182 @default.
- W2895072689 hasRelatedWork W2146647007 @default.
- W2895072689 hasRelatedWork W2256298421 @default.
- W2895072689 hasRelatedWork W2895072689 @default.
- W2895072689 hasRelatedWork W4246374982 @default.
- W2895072689 hasRelatedWork W2013834086 @default.
- W2895072689 isParatext "false" @default.
- W2895072689 isRetracted "false" @default.
- W2895072689 magId "2895072689" @default.
- W2895072689 workType "article" @default.