FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895163910> ?p ?o ?g. }

• W2895163910 endingPage "198" @default.
• W2895163910 startingPage "187" @default.
• W2895163910 abstract "Hypoglycaemia is a major barrier to good glucose control in type 1 diabetes. Frequent hypoglycaemic episodes impair awareness of subsequent hypoglycaemic bouts. Neural changes underpinning awareness of hypoglycaemia are poorly defined and molecular mechanisms by which glial cells contribute to hypoglycaemia sensing and glucose counterregulation require further investigation. The aim of the current study was to examine whether, and by what mechanism, human primary astrocyte (HPA) function was altered by acute and recurrent low glucose (RLG).To test whether glia, specifically astrocytes, could detect changes in glucose, we utilised HPA and U373 astrocytoma cells and exposed them to RLG in vitro. This allowed measurement, with high specificity and sensitivity, of RLG-associated changes in cellular metabolism. We examined changes in protein phosphorylation/expression using western blotting. Metabolic function was assessed using a Seahorse extracellular flux analyser. Immunofluorescent imaging was used to examine cell morphology and enzymatic assays were used to measure lactate release, glycogen content, intracellular ATP and nucleotide ratios.AMP-activated protein kinase (AMPK) was activated over a pathophysiologically relevant glucose concentration range. RLG produced an increased dependency on fatty acid oxidation for basal mitochondrial metabolism and exhibited hallmarks of mitochondrial stress, including increased proton leak and reduced coupling efficiency. Relative to glucose availability, lactate release increased during low glucose but this was not modified by RLG. Basal glucose uptake was not modified by RLG and glycogen levels were similar in control and RLG-treated cells. Mitochondrial adaptations to RLG were partially recovered by maintaining euglycaemic levels of glucose following RLG exposure.Taken together, these data indicate that HPA mitochondria are altered following RLG, with a metabolic switch towards increased fatty acid oxidation, suggesting glial adaptations to RLG involve altered mitochondrial metabolism that could contribute to defective glucose counterregulation to hypoglycaemia in diabetes." @default.
• W2895163910 created "2018-10-12" @default.
• W2895163910 creator A5004572173 @default.
• W2895163910 creator A5018828484 @default.
• W2895163910 creator A5021794854 @default.
• W2895163910 creator A5025149007 @default.
• W2895163910 creator A5030363523 @default.
• W2895163910 creator A5050133571 @default.
• W2895163910 creator A5081730635 @default.
• W2895163910 creator A5088002440 @default.
• W2895163910 date "2018-10-06" @default.
• W2895163910 modified "2023-10-17" @default.
• W2895163910 title "Basal fatty acid oxidation increases after recurrent low glucose in human primary astrocytes" @default.
• W2895163910 cites W1515550820 @default.
• W2895163910 cites W1928529925 @default.
• W2895163910 cites W1934303746 @default.
• W2895163910 cites W1985994475 @default.
• W2895163910 cites W1987344752 @default.
• W2895163910 cites W1988787774 @default.
• W2895163910 cites W1989236011 @default.
• W2895163910 cites W1992423721 @default.
• W2895163910 cites W1994454596 @default.
• W2895163910 cites W2001725826 @default.
• W2895163910 cites W2002865586 @default.
• W2895163910 cites W2040738597 @default.
• W2895163910 cites W2042767418 @default.
• W2895163910 cites W2043403270 @default.
• W2895163910 cites W2043485791 @default.
• W2895163910 cites W2045838525 @default.
• W2895163910 cites W2047790518 @default.
• W2895163910 cites W2049871916 @default.
• W2895163910 cites W2070993610 @default.
• W2895163910 cites W2077335475 @default.
• W2895163910 cites W2077779198 @default.
• W2895163910 cites W2087561593 @default.
• W2895163910 cites W2096209210 @default.
• W2895163910 cites W2096591765 @default.
• W2895163910 cites W2112181364 @default.
• W2895163910 cites W2122400789 @default.
• W2895163910 cites W2130634848 @default.
• W2895163910 cites W2132018095 @default.
• W2895163910 cites W2133572469 @default.
• W2895163910 cites W2134271030 @default.
• W2895163910 cites W2141999573 @default.
• W2895163910 cites W2148271857 @default.
• W2895163910 cites W2153116158 @default.
• W2895163910 cites W2154722810 @default.
• W2895163910 cites W2161639078 @default.
• W2895163910 cites W2165907640 @default.
• W2895163910 cites W2169041612 @default.
• W2895163910 cites W2463803438 @default.
• W2895163910 cites W2486865141 @default.
• W2895163910 cites W2512005241 @default.
• W2895163910 cites W2533146972 @default.
• W2895163910 cites W2541802021 @default.
• W2895163910 cites W2560136455 @default.
• W2895163910 cites W2588929868 @default.
• W2895163910 cites W2607436680 @default.
• W2895163910 cites W2782274120 @default.
• W2895163910 cites W2790149673 @default.
• W2895163910 cites W2791406808 @default.
• W2895163910 cites W2593595447 @default.
• W2895163910 doi "https://doi.org/10.1007/s00125-018-4744-6" @default.
• W2895163910 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6290858" @default.
• W2895163910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30293112" @default.
• W2895163910 hasPublicationYear "2018" @default.
• W2895163910 type Work @default.
• W2895163910 sameAs 2895163910 @default.
• W2895163910 citedByCount "22" @default.
• W2895163910 countsByYear W28951639102019 @default.
• W2895163910 countsByYear W28951639102020 @default.
• W2895163910 countsByYear W28951639102021 @default.
• W2895163910 countsByYear W28951639102022 @default.
• W2895163910 countsByYear W28951639102023 @default.
• W2895163910 crossrefType "journal-article" @default.
• W2895163910 hasAuthorship W2895163910A5004572173 @default.
• W2895163910 hasAuthorship W2895163910A5018828484 @default.
• W2895163910 hasAuthorship W2895163910A5021794854 @default.
• W2895163910 hasAuthorship W2895163910A5025149007 @default.
• W2895163910 hasAuthorship W2895163910A5030363523 @default.
• W2895163910 hasAuthorship W2895163910A5050133571 @default.
• W2895163910 hasAuthorship W2895163910A5081730635 @default.
• W2895163910 hasAuthorship W2895163910A5088002440 @default.
• W2895163910 hasBestOaLocation W28951639101 @default.
• W2895163910 hasConcept C126322002 @default.
• W2895163910 hasConcept C134018914 @default.
• W2895163910 hasConcept C161573976 @default.
• W2895163910 hasConcept C17093226 @default.
• W2895163910 hasConcept C2777499176 @default.
• W2895163910 hasConcept C2777542381 @default.
• W2895163910 hasConcept C2777866211 @default.
• W2895163910 hasConcept C2778024521 @default.
• W2895163910 hasConcept C2779306644 @default.
• W2895163910 hasConcept C28406088 @default.
• W2895163910 hasConcept C529278444 @default.
• W2895163910 hasConcept C55493867 @default.
• W2895163910 hasConcept C71924100 @default.
• W2895163910 hasConcept C86803240 @default.

• next