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• W2895528841 startingPage "11618" @default.
• W2895528841 abstract "From gene expression to nanotechnology, understanding and controlling DNA requires a detailed knowledge of its higher order structure and dynamics. Here we take advantage of the environment-sensitive photoisomerization of cyanine dyes to probe local and global changes in DNA structure. We report that a covalently attached Cy3 dye undergoes strong enhancement of fluorescence intensity and lifetime when stacked in a nick, gap or overhang region in duplex DNA. This is used to probe hybridization dynamics of a DNA hairpin down to the single-molecule level. We also show that varying the position of a single abasic site up to 20 base pairs away modulates the dye-DNA interaction, indicative of through-backbone allosteric interactions. The phenomenon of stacking-induced fluorescence increase (SIFI) should find widespread use in the study of the structure, dynamics and reactivity of nucleic acids." @default.
• W2895528841 created "2018-10-12" @default.
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• W2895528841 date "2018-10-02" @default.
• W2895528841 modified "2023-10-16" @default.
• W2895528841 title "Stacking-induced fluorescence increase reveals allosteric interactions through DNA" @default.
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• W2895528841 doi "https://doi.org/10.1093/nar/gky887" @default.
• W2895528841 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6265455" @default.
• W2895528841 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30277520" @default.
• W2895528841 hasPublicationYear "2018" @default.
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