Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895538528> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W2895538528 abstract "G protein-coupled receptors (GPCRs) constitute the largest human membrane protein superfamily with over 800 members. They reside in plasma membranes mediating cell signaling and regulating the majority of physiological processes, making them successful targets of about 30–40% of current drugs. Detailed understanding of the mechanism of GPCR action requires high-resolution structural information for many representative members of the family captured in different conformational states, but progress has been limited by the difficulty of preparing large amounts of homogenous and stable samples and by the need for sufficiently large crystals. In this chapter we summarize the work that led to the development and successful application of serial femtosecond crystallography (SFX) to micron-sized GPCR crystals grown and delivered to an X-ray free electron laser (XFEL) beam in a lipid membrane mimetic mesophase, known as lipidic cubic phase (LCP). The LCP-SFX approach obviates the need for growing larger crystals, while also minimizing radiation damage effects on room-temperature samples, and so could substantially shorten the time to success, with concomitant significant reduction in cost and effort. It therefore has the potential to revolutionize structural studies on challenging membrane proteins and their complexes with soluble partners." @default.
- W2895538528 created "2018-10-12" @default.
- W2895538528 creator A5042343928 @default.
- W2895538528 creator A5050336210 @default.
- W2895538528 creator A5052806121 @default.
- W2895538528 creator A5063729267 @default.
- W2895538528 date "2018-01-01" @default.
- W2895538528 modified "2023-09-27" @default.
- W2895538528 title "Chapter 2. Delivery of GPCR Crystals for Serial Femtosecond Crystallography" @default.
- W2895538528 doi "https://doi.org/10.1039/9781788010504-00028" @default.
- W2895538528 hasPublicationYear "2018" @default.
- W2895538528 type Work @default.
- W2895538528 sameAs 2895538528 @default.
- W2895538528 citedByCount "0" @default.
- W2895538528 crossrefType "book-chapter" @default.
- W2895538528 hasAuthorship W2895538528A5042343928 @default.
- W2895538528 hasAuthorship W2895538528A5050336210 @default.
- W2895538528 hasAuthorship W2895538528A5052806121 @default.
- W2895538528 hasAuthorship W2895538528A5063729267 @default.
- W2895538528 hasConcept C120665830 @default.
- W2895538528 hasConcept C121332964 @default.
- W2895538528 hasConcept C12554922 @default.
- W2895538528 hasConcept C135285700 @default.
- W2895538528 hasConcept C144647389 @default.
- W2895538528 hasConcept C167735695 @default.
- W2895538528 hasConcept C170493617 @default.
- W2895538528 hasConcept C171250308 @default.
- W2895538528 hasConcept C185592680 @default.
- W2895538528 hasConcept C192562407 @default.
- W2895538528 hasConcept C41625074 @default.
- W2895538528 hasConcept C520434653 @default.
- W2895538528 hasConcept C55493867 @default.
- W2895538528 hasConcept C60644358 @default.
- W2895538528 hasConcept C8010536 @default.
- W2895538528 hasConcept C86803240 @default.
- W2895538528 hasConcept C92028520 @default.
- W2895538528 hasConceptScore W2895538528C120665830 @default.
- W2895538528 hasConceptScore W2895538528C121332964 @default.
- W2895538528 hasConceptScore W2895538528C12554922 @default.
- W2895538528 hasConceptScore W2895538528C135285700 @default.
- W2895538528 hasConceptScore W2895538528C144647389 @default.
- W2895538528 hasConceptScore W2895538528C167735695 @default.
- W2895538528 hasConceptScore W2895538528C170493617 @default.
- W2895538528 hasConceptScore W2895538528C171250308 @default.
- W2895538528 hasConceptScore W2895538528C185592680 @default.
- W2895538528 hasConceptScore W2895538528C192562407 @default.
- W2895538528 hasConceptScore W2895538528C41625074 @default.
- W2895538528 hasConceptScore W2895538528C520434653 @default.
- W2895538528 hasConceptScore W2895538528C55493867 @default.
- W2895538528 hasConceptScore W2895538528C60644358 @default.
- W2895538528 hasConceptScore W2895538528C8010536 @default.
- W2895538528 hasConceptScore W2895538528C86803240 @default.
- W2895538528 hasConceptScore W2895538528C92028520 @default.
- W2895538528 hasLocation W28955385281 @default.
- W2895538528 hasOpenAccess W2895538528 @default.
- W2895538528 hasPrimaryLocation W28955385281 @default.
- W2895538528 hasRelatedWork W1493067599 @default.
- W2895538528 hasRelatedWork W1972621135 @default.
- W2895538528 hasRelatedWork W2089581179 @default.
- W2895538528 hasRelatedWork W2145372796 @default.
- W2895538528 hasRelatedWork W2243756977 @default.
- W2895538528 hasRelatedWork W3171966215 @default.
- W2895538528 isParatext "false" @default.
- W2895538528 isRetracted "false" @default.
- W2895538528 magId "2895538528" @default.
- W2895538528 workType "book-chapter" @default.