Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895567219> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W2895567219 endingPage "269" @default.
- W2895567219 startingPage "269" @default.
- W2895567219 abstract "Collagen, an important component of the extracellular matrix and produced by dermal fibroblasts, is the key to healthy and firm skin. However, the ability of the fibroblast to produce a sufficient amount of collagen is depleted with age. Collagen hydrolysate (CH) is the product of extensive degradation of gelatine. In the present study, CH of molecular ranging 1-5kDa and its related compounds were evaluated for their effect on cell proliferation and matrix production of cultured human dermal fibroblasts (HDF). Two age classes of HDF were prepared, namely the normal HDF and senescence acceleration HDF, induced by hydrogen peroxide (50μM) treatment. CH was added to both classes at different concentrations (1μg/mL, 10μg/ml, 100μg/ml and 1000μg/ml). The cell proliferation was measured using WST-1 assay and the matrix production was evaluated by alcian blue (AB) pH 2.5. An additional assay, the β-galactosidase activity assay (SA-β-Gal), was measured to evaluate the degree of cellular senescence. The cell proliferation was measured using WST-1 assay and matrix production was measured with alcian blue (AB) pH2.5. The results revealed that cell proliferation had significant differences in both normal HDF and senescence-induced HDF. Beta-Galactosidase results revealed a positive decline of senescent cells at higher concentrations of CH (100 & 1000 μg/L). The Alcian Blue pH2.5 showed contrasting results in normal HDF (p<0.05) at 100 & 1000 μg/L and senescence-induced HDF showed no significant differences. This led to the conclusion that a higher CH concentration is able to produce significant differences in both normal and senescence HDF on cell proliferation and matrix production." @default.
- W2895567219 created "2018-10-12" @default.
- W2895567219 creator A5007666663 @default.
- W2895567219 creator A5020331427 @default.
- W2895567219 creator A5025085233 @default.
- W2895567219 creator A5050081622 @default.
- W2895567219 creator A5051864047 @default.
- W2895567219 date "2018-01-01" @default.
- W2895567219 modified "2023-09-27" @default.
- W2895567219 title "Effect of Collagen-Related compound on cell proliferation and matrix production with cultured human dermal fibroblasts" @default.
- W2895567219 doi "https://doi.org/10.5958/0974-4614.2018.00083.9" @default.
- W2895567219 hasPublicationYear "2018" @default.
- W2895567219 type Work @default.
- W2895567219 sameAs 2895567219 @default.
- W2895567219 citedByCount "4" @default.
- W2895567219 countsByYear W28955672192022 @default.
- W2895567219 countsByYear W28955672192023 @default.
- W2895567219 crossrefType "journal-article" @default.
- W2895567219 hasAuthorship W2895567219A5007666663 @default.
- W2895567219 hasAuthorship W2895567219A5020331427 @default.
- W2895567219 hasAuthorship W2895567219A5025085233 @default.
- W2895567219 hasAuthorship W2895567219A5050081622 @default.
- W2895567219 hasAuthorship W2895567219A5051864047 @default.
- W2895567219 hasConcept C105702510 @default.
- W2895567219 hasConcept C106487976 @default.
- W2895567219 hasConcept C185592680 @default.
- W2895567219 hasConcept C2780381497 @default.
- W2895567219 hasConcept C43617362 @default.
- W2895567219 hasConcept C54355233 @default.
- W2895567219 hasConcept C81885089 @default.
- W2895567219 hasConcept C86803240 @default.
- W2895567219 hasConcept C95444343 @default.
- W2895567219 hasConceptScore W2895567219C105702510 @default.
- W2895567219 hasConceptScore W2895567219C106487976 @default.
- W2895567219 hasConceptScore W2895567219C185592680 @default.
- W2895567219 hasConceptScore W2895567219C2780381497 @default.
- W2895567219 hasConceptScore W2895567219C43617362 @default.
- W2895567219 hasConceptScore W2895567219C54355233 @default.
- W2895567219 hasConceptScore W2895567219C81885089 @default.
- W2895567219 hasConceptScore W2895567219C86803240 @default.
- W2895567219 hasConceptScore W2895567219C95444343 @default.
- W2895567219 hasIssue "3and4" @default.
- W2895567219 hasLocation W28955672191 @default.
- W2895567219 hasOpenAccess W2895567219 @default.
- W2895567219 hasPrimaryLocation W28955672191 @default.
- W2895567219 hasRelatedWork W1987714793 @default.
- W2895567219 hasRelatedWork W2007948417 @default.
- W2895567219 hasRelatedWork W2009991832 @default.
- W2895567219 hasRelatedWork W2031508871 @default.
- W2895567219 hasRelatedWork W2080002581 @default.
- W2895567219 hasRelatedWork W2336009021 @default.
- W2895567219 hasRelatedWork W2355138112 @default.
- W2895567219 hasRelatedWork W2416367974 @default.
- W2895567219 hasRelatedWork W4362542844 @default.
- W2895567219 hasRelatedWork W4362547836 @default.
- W2895567219 hasVolume "21" @default.
- W2895567219 isParatext "false" @default.
- W2895567219 isRetracted "false" @default.
- W2895567219 magId "2895567219" @default.
- W2895567219 workType "article" @default.