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• W2895588890 abstract "Background Hepatitis C Virus HCV infection is a global public health problem and a leading cause for liver cirrhosis and hepatocellular carcinoma HCV is a single stranded RNA virus of the Flaviviridae family with genotypes and subtypes Viral genotypes viral loads determine potential response duration and outcome of therapy This study aimed to determine biochemical aberrations and HCV viral load genotypic patterns among Sudanese patients with chronic HCV infection Methods Following informed consent four hundreds and seventy three patients with ELISA reactivity to HCV were enrolled Patients with chronic alcohol consumption Hepatitis B virus or HIV co infections were excluded Plasma was collected for Albumin Globulin Alkaline phosphatase AST ALT levels bilirubin and HCV viral load genotypes determination using commercial kits chemistry analyzer qPCR machine Briefly Total RNA extraction reverse transcription to cDNA on RNA template using sequence specific primers a positive control for standard curve construction and Internal Control to rule out possible false negative results to monitor analysis steps and to reveal the effect of PCR inhibitors Results Patients rsquo mean age was plusmn years and a Male Female ratio of Mean serum albumin plusmn grams dL and globulin plusmn grams dL levels were within normal range While alkaline phosphatase plusmn IU L p lt ALT plusmn U L p lt AST plusmn U L p lt total bilirubin plusmn mg dl p lt and direct bilirubin plusmn mg dL p lt were significantly elevated HCV viral loads of to IU mL Median IU mL were detected Genotype a was detected in the majority of patients while genotypes a and b were reported in a minority each HCV genotypes a and a b had similar ALT AST bilirubin levels and baseline viral loads Conclusion HCV genotype was the most common genotype in Sudan while genotypes a and b were seen in a minority There were no significant correlations between HCV genotypes and serum ALT AST levels baseline HCV viral loads" @default.
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• W2895588890 date "2018-01-01" @default.
• W2895588890 modified "2023-10-18" @default.
• W2895588890 title "Biochemical aberrations, viral genotypic patterns and viral loads among Sudanese patients with chronic hepatitis C virus infection" @default.
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• W2895588890 doi "https://doi.org/10.15406/jhvrv.2018.06.00195" @default.
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