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- W2895598655 abstract "We implemented a completely label-free biophysical (morphometric and optical) property characterization of living monocytes in flow, using measurements obtained from two coherent imaging techniques: a pure light scattering approach to obtain an optical signature (OS) of cells, and a digital holography (DH) approach to achieve optical cell reconstructions in flow. A precise 3D cell alignment platform, taking advantage of viscoelastic fluid properties and microfluidic channel geometry, was used to investigate the OS of cells to achieve their refractive index, ratio of the nucleus over cytoplasm, and overall cell dimension. Further quantitative phase-contrast reconstructions by DH were employed to calculate surface area, dry mass, and biovolume of monocytes by using the OS outcomes as input parameters. The results show significantly different biophysical cell properties, confirming the possibility to differentiate monocytes from other cell classes in flow, thus avoiding chemical cell staining or labeling, which are nowadays used." @default.
- W2895598655 created "2018-10-12" @default.
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- W2895598655 date "2018-10-04" @default.
- W2895598655 modified "2023-10-01" @default.
- W2895598655 title "Biophysical investigation of living monocytes in flow by collaborative coherent imaging techniques" @default.
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- W2895598655 doi "https://doi.org/10.1364/boe.9.005194" @default.
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