FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895781428> ?p ?o ?g. }

• W2895781428 endingPage "399" @default.
• W2895781428 startingPage "387" @default.
• W2895781428 abstract "<b><i>Background and Objectives:</i></b> Elevated levels of saturated fatty acids (SFA) induce a state of neuroinflammation in the hypothalamus. It has been suggested that microglia sense palmitate, a prevalent circulating SFA, and act as mediators of this inflammatory process by communicating with neurons, particularly those involved in appetite regulation. In this study, we examined the inflammatory response to palmitate in immortalized microglial cell lines, BV-2 and IMG, and the subsequent effects on inflammatory gene expression in a model of NPY/AgRP neurons, mHypoE-46. <b><i>Methods:</i></b> The BV-2 cells were treated with 50 µM palmitate for 4 and 24 h, and the transcriptional regulation of markers for inflammation and cellular stress was assessed using an RT² Profiler PCR Array. Select genes were verified with qRT-PCR. The BV-2 and IMG cells were then co-cultured using 1.0-µm cell culture inserts with an immortalized hypothalamic cell line, mHypoE-46, to investigate potential intercellular communication between microglia and neurons. <b><i>Results:</i></b> We found that palmitate increased the mRNA levels of specific inflammatory genes, and a general anti-inflammatory profile was revealed in the microglia cells. The mRNA changes in TNFα at 4 and 24 h in BV-2 cells were abrogated with the toll-like receptor 4 (TLR4) inhibitor, TAK-242, indicating the involvement of TLR4. Co-culture of mHypoE-46 neurons with microglia pre-treated with palmitate resulted in repression of TNFα expression in the hypothalamic neurons. As palmitate significantly increased IL-13 expression in microglia, the effect of this cytokine was tested in mHypoE-46 neurons. The addition of IL-13 to neuronal cultures normalized the palmitate-mediated increase in IL-6 and AgRP expression, suggesting that microglia may protect surrounding neurons, at least in part, through the release of IL-13. <b><i>Conclusions:</i></b> These results suggest a potential anti-inflammatory role of microglia towards the palmitate-induced neuroinflammation, and potentially energy homeostasis, in hypothalamic neurons." @default.
• W2895781428 created "2018-10-26" @default.
• W2895781428 creator A5048687492 @default.
• W2895781428 creator A5059717484 @default.
• W2895781428 creator A5078504107 @default.
• W2895781428 creator A5081629872 @default.
• W2895781428 date "2018-01-01" @default.
• W2895781428 modified "2023-09-26" @default.
• W2895781428 title "Palmitate Induces an Anti-Inflammatory Response in Immortalized Microglial BV-2 and IMG Cell Lines that Decreases TNFα Levels in mHypoE-46 Hypothalamic Neurons in Co-Culture" @default.
• W2895781428 cites W1548862642 @default.
• W2895781428 cites W1576120369 @default.
• W2895781428 cites W1768846296 @default.
• W2895781428 cites W1806065864 @default.
• W2895781428 cites W1964562779 @default.
• W2895781428 cites W1965102835 @default.
• W2895781428 cites W1965513983 @default.
• W2895781428 cites W1974031490 @default.
• W2895781428 cites W1982316939 @default.
• W2895781428 cites W1997308909 @default.
• W2895781428 cites W1997773716 @default.
• W2895781428 cites W1998840552 @default.
• W2895781428 cites W2000644415 @default.
• W2895781428 cites W2011361113 @default.
• W2895781428 cites W2012730798 @default.
• W2895781428 cites W2015571684 @default.
• W2895781428 cites W2017712441 @default.
• W2895781428 cites W2021654494 @default.
• W2895781428 cites W2024252214 @default.
• W2895781428 cites W2025131475 @default.
• W2895781428 cites W2026828902 @default.
• W2895781428 cites W2028852111 @default.
• W2895781428 cites W2029917664 @default.
• W2895781428 cites W2040352209 @default.
• W2895781428 cites W2040423913 @default.
• W2895781428 cites W2050782923 @default.
• W2895781428 cites W2064762514 @default.
• W2895781428 cites W2067875627 @default.
• W2895781428 cites W2069893872 @default.
• W2895781428 cites W2070197168 @default.
• W2895781428 cites W2071683156 @default.
• W2895781428 cites W2073861682 @default.
• W2895781428 cites W2081058440 @default.
• W2895781428 cites W2086357418 @default.
• W2895781428 cites W2087736278 @default.
• W2895781428 cites W2087813533 @default.
• W2895781428 cites W2090817128 @default.
• W2895781428 cites W2092564896 @default.
• W2895781428 cites W2101898118 @default.
• W2895781428 cites W2112292567 @default.
• W2895781428 cites W2127096777 @default.
• W2895781428 cites W2130440272 @default.
• W2895781428 cites W2151335106 @default.
• W2895781428 cites W2154349779 @default.
• W2895781428 cites W2154696002 @default.
• W2895781428 cites W2157137975 @default.
• W2895781428 cites W2159181646 @default.
• W2895781428 cites W2160447524 @default.
• W2895781428 cites W2164594351 @default.
• W2895781428 cites W2281054724 @default.
• W2895781428 cites W2294718904 @default.
• W2895781428 cites W2316493884 @default.
• W2895781428 cites W2340761255 @default.
• W2895781428 cites W2402173887 @default.
• W2895781428 cites W2406777542 @default.
• W2895781428 cites W2535218491 @default.
• W2895781428 cites W2557380874 @default.
• W2895781428 cites W2564256804 @default.
• W2895781428 cites W333643404 @default.
• W2895781428 doi "https://doi.org/10.1159/000494759" @default.
• W2895781428 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30352432" @default.
• W2895781428 hasPublicationYear "2018" @default.
• W2895781428 type Work @default.
• W2895781428 sameAs 2895781428 @default.
• W2895781428 citedByCount "19" @default.
• W2895781428 countsByYear W28957814282019 @default.
• W2895781428 countsByYear W28957814282020 @default.
• W2895781428 countsByYear W28957814282021 @default.
• W2895781428 countsByYear W28957814282022 @default.
• W2895781428 countsByYear W28957814282023 @default.
• W2895781428 crossrefType "journal-article" @default.
• W2895781428 hasAuthorship W2895781428A5048687492 @default.
• W2895781428 hasAuthorship W2895781428A5059717484 @default.
• W2895781428 hasAuthorship W2895781428A5078504107 @default.
• W2895781428 hasAuthorship W2895781428A5081629872 @default.
• W2895781428 hasConcept C126322002 @default.
• W2895781428 hasConcept C134018914 @default.
• W2895781428 hasConcept C153911025 @default.
• W2895781428 hasConcept C170493617 @default.
• W2895781428 hasConcept C17991360 @default.
• W2895781428 hasConcept C185592680 @default.
• W2895781428 hasConcept C2776070231 @default.
• W2895781428 hasConcept C2776914184 @default.
• W2895781428 hasConcept C2777003273 @default.
• W2895781428 hasConcept C2779830541 @default.
• W2895781428 hasConcept C54355233 @default.
• W2895781428 hasConcept C71924100 @default.
• W2895781428 hasConcept C81885089 @default.
• W2895781428 hasConcept C86803240 @default.

• next