FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895801152> ?p ?o ?g. }

• W2895801152 endingPage "910" @default.
• W2895801152 startingPage "901" @default.
• W2895801152 abstract "Damaged articular cartilage has limited healing capacity due to the lack of blood supply. Tissue engineering using the chondrogenic potential of mesenchymal stem cells (MSCs) is a promising approach for cartilage regenerative therapy. Canines are a relevant animal model for cartilage regeneration, as they suffer from spontaneous cartilage injury similar to humans. However, the chondrogenic capacity and optimal conditions for chondrogenesis in canine MSCs have not been documented. In this study, we investigated the effect of preconditioning with fibroblast growth factor-2 (FGF-2) and fetal bovine serum (FBS) in chondrogenic induction medium on canine MSC chondrogenesis. Our results show that treatment with FGF-2 promoted cell proliferation and the expression of SOX2. Biochemical and histological analyses revealed that higher concentrations of FBS decreased chondrogenesis of canine MSCs expanded without FGF-2. However, cells expanded with FGF-2 showed substantial chondrogenic potential and produced abundant cartilage matrix, even on FBS addition. Quantitative polymerase chain reaction showed that chondrogenic genes were highly expressed in spheroids, when cells were expanded with FGF-2 under serum-free conditions. Thus, the combination of FGF-2 preconditioning and serum-free chondrogenic induction medium enabled efficient chondrogenic differentiation of canine MSCs and contributed to cartilage regeneration research. Tissue engineering using the chondrogenic potential of mesenchymal stem cells (MSCs) is a promising approach for cartilage regenerative therapy. Although dogs are widely used as an animal model for cartilage regeneration, chondrogenic differentiation of canine MSCs is still challenging. In this study, we aimed at establishing the optimal conditions for canine MSC chondrogenesis. Our results demonstrated that preconditioning with fibroblast growth factor-2 and serum-free induction medium enabled robust chondrogenesis of canine MSCs. These findings will allow effective generation of cartilage tissue from canine MSCs and advance research of cartilage regeneration in both dogs and humans." @default.
• W2895801152 created "2018-10-26" @default.
• W2895801152 creator A5033262704 @default.
• W2895801152 creator A5034118045 @default.
• W2895801152 creator A5061183864 @default.
• W2895801152 creator A5084255017 @default.
• W2895801152 date "2019-06-01" @default.
• W2895801152 modified "2023-10-02" @default.
• W2895801152 title "Effect of Fibroblast Growth Factor-2 and Serum on Canine Mesenchymal Stem Cell Chondrogenesis" @default.
• W2895801152 cites W1525482295 @default.
• W2895801152 cites W1903268454 @default.
• W2895801152 cites W1929251602 @default.
• W2895801152 cites W1980839053 @default.
• W2895801152 cites W1985082605 @default.
• W2895801152 cites W1988592246 @default.
• W2895801152 cites W1989375600 @default.
• W2895801152 cites W1994157958 @default.
• W2895801152 cites W2003998378 @default.
• W2895801152 cites W2005111784 @default.
• W2895801152 cites W2012052877 @default.
• W2895801152 cites W2021556708 @default.
• W2895801152 cites W2029387074 @default.
• W2895801152 cites W2030493675 @default.
• W2895801152 cites W2032282401 @default.
• W2895801152 cites W2032372303 @default.
• W2895801152 cites W2049791447 @default.
• W2895801152 cites W2066687881 @default.
• W2895801152 cites W2070565156 @default.
• W2895801152 cites W2074541678 @default.
• W2895801152 cites W2077515150 @default.
• W2895801152 cites W2084106798 @default.
• W2895801152 cites W2085625304 @default.
• W2895801152 cites W2093265167 @default.
• W2895801152 cites W2094789114 @default.
• W2895801152 cites W2096467350 @default.
• W2895801152 cites W2121960517 @default.
• W2895801152 cites W2129262362 @default.
• W2895801152 cites W2131597955 @default.
• W2895801152 cites W2132610511 @default.
• W2895801152 cites W2137087836 @default.
• W2895801152 cites W2150488245 @default.
• W2895801152 cites W2152861671 @default.
• W2895801152 cites W2154555172 @default.
• W2895801152 cites W2162823309 @default.
• W2895801152 cites W2171540322 @default.
• W2895801152 cites W2172257496 @default.
• W2895801152 cites W2471198936 @default.
• W2895801152 cites W2558593042 @default.
• W2895801152 cites W2582860253 @default.
• W2895801152 doi "https://doi.org/10.1089/ten.tea.2018.0177" @default.
• W2895801152 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30319056" @default.
• W2895801152 hasPublicationYear "2019" @default.
• W2895801152 type Work @default.
• W2895801152 sameAs 2895801152 @default.
• W2895801152 citedByCount "16" @default.
• W2895801152 countsByYear W28958011522019 @default.
• W2895801152 countsByYear W28958011522020 @default.
• W2895801152 countsByYear W28958011522021 @default.
• W2895801152 countsByYear W28958011522022 @default.
• W2895801152 countsByYear W28958011522023 @default.
• W2895801152 crossrefType "journal-article" @default.
• W2895801152 hasAuthorship W2895801152A5033262704 @default.
• W2895801152 hasAuthorship W2895801152A5034118045 @default.
• W2895801152 hasAuthorship W2895801152A5061183864 @default.
• W2895801152 hasAuthorship W2895801152A5084255017 @default.
• W2895801152 hasBestOaLocation W28958011521 @default.
• W2895801152 hasConcept C105702510 @default.
• W2895801152 hasConcept C136229726 @default.
• W2895801152 hasConcept C142724271 @default.
• W2895801152 hasConcept C170493617 @default.
• W2895801152 hasConcept C171056886 @default.
• W2895801152 hasConcept C185592680 @default.
• W2895801152 hasConcept C198826908 @default.
• W2895801152 hasConcept C203014093 @default.
• W2895801152 hasConcept C204787440 @default.
• W2895801152 hasConcept C2776164576 @default.
• W2895801152 hasConcept C2778232976 @default.
• W2895801152 hasConcept C2780550940 @default.
• W2895801152 hasConcept C35496256 @default.
• W2895801152 hasConcept C49892992 @default.
• W2895801152 hasConcept C55493867 @default.
• W2895801152 hasConcept C71924100 @default.
• W2895801152 hasConcept C74373430 @default.
• W2895801152 hasConcept C86803240 @default.
• W2895801152 hasConcept C95444343 @default.
• W2895801152 hasConceptScore W2895801152C105702510 @default.
• W2895801152 hasConceptScore W2895801152C136229726 @default.
• W2895801152 hasConceptScore W2895801152C142724271 @default.
• W2895801152 hasConceptScore W2895801152C170493617 @default.
• W2895801152 hasConceptScore W2895801152C171056886 @default.
• W2895801152 hasConceptScore W2895801152C185592680 @default.
• W2895801152 hasConceptScore W2895801152C198826908 @default.
• W2895801152 hasConceptScore W2895801152C203014093 @default.
• W2895801152 hasConceptScore W2895801152C204787440 @default.
• W2895801152 hasConceptScore W2895801152C2776164576 @default.
• W2895801152 hasConceptScore W2895801152C2778232976 @default.
• W2895801152 hasConceptScore W2895801152C2780550940 @default.
• W2895801152 hasConceptScore W2895801152C35496256 @default.

• next