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- W2895804975 abstract "The use of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) has become commonplace in the study of neuronal development, physiology, disease modelling, and therapy development. Due to the transient nature of working with these cells, it is important to regularly confirm the cell status as a naive stem cell versus a more defined neural progenitor cell (NPC). Classically, this has been done using a panel of specific antibodies to test for the expression of transcription factors known to be observed in ESCs, but not NPCs. However, this method is both time consuming and expensive. Here, we describe the use of the NanoString nCounter system for determining the levels of expression of key transcription factors that will effectively aid in determining the state of your stem cell cultures." @default.
- W2895804975 created "2018-10-26" @default.
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- W2895804975 date "2018-10-16" @default.
- W2895804975 modified "2023-09-30" @default.
- W2895804975 title "Detecting Stem Cell Marker Expression Using the NanoString nCounter System" @default.
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- W2895804975 doi "https://doi.org/10.1007/978-1-4939-8805-1_5" @default.
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