Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895827741> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2895827741 abstract "Downstream processing of immunoglobulin G is conventionally performed by bead-based column chromatography; affinity chromatography with Protein A is the most selective process in biotechnological industry for the purification of antibodies. However, this method suffers from several limitations, such as high pressure drops across the column, compaction of the granular porous bed and low diffusivity between the particles. In addition to fluid dynamic problems, the traditional process is affected by limitations related to the use of Protein A as affinity ligand. Materials activated with Protein A are very expensive, since the production cost of this recombinant protein is very high. Finally, ligand leakage causes contamination of the final product.The aim of the present work was to overcome the limitations that affect the traditional process through the development of new convective chromatographic supports, like membranes and monoliths, functionalized with synthetic affinity ligands to be used instead of columns packed with Protein A beads.The performance of regenerated cellulose membranes functionalized with two affinity ligands were studied; the commercial A2P ligand and the new recently synthesized HPTA were used to replace Protein A. Static and dynamic binding capacity and elution recoveries, were determined for the affinity membranes prepared, using pure solutions of IgG. Mixtures and complex solutions containing IgG were tested to determine ligands selectivity.A processing method for the preparation of porous cellular ceramic monoliths was developed. Cellular Al2TiO5 and Al2TiO5-Al2O3 composites ceramics were obtained by emulsification of liquid paraffin in aqueous suspensions of mixed Al2O3 and TiO2 powders, with subsequent burnout of the organic phase and two-step reactive firing.The ceramic monolith were fully characterized to assess their use as novel chromatographic stationary phases, measuring porosity, axial dispersion coefficient and permeability; the surface was chemically modified to obtain functional groups to be used for ligand or direct protein immobilization." @default.
- W2895827741 created "2018-10-26" @default.
- W2895827741 creator A5020392985 @default.
- W2895827741 creator A5029813068 @default.
- W2895827741 creator A5052537040 @default.
- W2895827741 creator A5076173987 @default.
- W2895827741 creator A5081117767 @default.
- W2895827741 date "2016-01-01" @default.
- W2895827741 modified "2023-09-23" @default.
- W2895827741 title "Purification of antibodies through convective chromatography" @default.
- W2895827741 doi "https://doi.org/10.6092/unibo/amsdottorato/8535" @default.
- W2895827741 hasPublicationYear "2016" @default.
- W2895827741 type Work @default.
- W2895827741 sameAs 2895827741 @default.
- W2895827741 citedByCount "0" @default.
- W2895827741 crossrefType "journal-article" @default.
- W2895827741 hasAuthorship W2895827741A5020392985 @default.
- W2895827741 hasAuthorship W2895827741A5029813068 @default.
- W2895827741 hasAuthorship W2895827741A5052537040 @default.
- W2895827741 hasAuthorship W2895827741A5076173987 @default.
- W2895827741 hasAuthorship W2895827741A5081117767 @default.
- W2895827741 hasConcept C116569031 @default.
- W2895827741 hasConcept C159654299 @default.
- W2895827741 hasConcept C170493617 @default.
- W2895827741 hasConcept C178790620 @default.
- W2895827741 hasConcept C179247698 @default.
- W2895827741 hasConcept C181199279 @default.
- W2895827741 hasConcept C185592680 @default.
- W2895827741 hasConcept C192562407 @default.
- W2895827741 hasConcept C203014093 @default.
- W2895827741 hasConcept C2779000548 @default.
- W2895827741 hasConcept C41625074 @default.
- W2895827741 hasConcept C43617362 @default.
- W2895827741 hasConcept C55493867 @default.
- W2895827741 hasConcept C6648577 @default.
- W2895827741 hasConcept C73826308 @default.
- W2895827741 hasConcept C86803240 @default.
- W2895827741 hasConcept C8985478 @default.
- W2895827741 hasConceptScore W2895827741C116569031 @default.
- W2895827741 hasConceptScore W2895827741C159654299 @default.
- W2895827741 hasConceptScore W2895827741C170493617 @default.
- W2895827741 hasConceptScore W2895827741C178790620 @default.
- W2895827741 hasConceptScore W2895827741C179247698 @default.
- W2895827741 hasConceptScore W2895827741C181199279 @default.
- W2895827741 hasConceptScore W2895827741C185592680 @default.
- W2895827741 hasConceptScore W2895827741C192562407 @default.
- W2895827741 hasConceptScore W2895827741C203014093 @default.
- W2895827741 hasConceptScore W2895827741C2779000548 @default.
- W2895827741 hasConceptScore W2895827741C41625074 @default.
- W2895827741 hasConceptScore W2895827741C43617362 @default.
- W2895827741 hasConceptScore W2895827741C55493867 @default.
- W2895827741 hasConceptScore W2895827741C6648577 @default.
- W2895827741 hasConceptScore W2895827741C73826308 @default.
- W2895827741 hasConceptScore W2895827741C86803240 @default.
- W2895827741 hasConceptScore W2895827741C8985478 @default.
- W2895827741 hasLocation W28958277411 @default.
- W2895827741 hasOpenAccess W2895827741 @default.
- W2895827741 hasPrimaryLocation W28958277411 @default.
- W2895827741 hasRelatedWork W187491976 @default.
- W2895827741 hasRelatedWork W1924734387 @default.
- W2895827741 hasRelatedWork W1970865372 @default.
- W2895827741 hasRelatedWork W1996451973 @default.
- W2895827741 hasRelatedWork W2053481244 @default.
- W2895827741 hasRelatedWork W2083309940 @default.
- W2895827741 hasRelatedWork W2089214373 @default.
- W2895827741 hasRelatedWork W2107186186 @default.
- W2895827741 hasRelatedWork W2135006591 @default.
- W2895827741 hasRelatedWork W2326898052 @default.
- W2895827741 hasRelatedWork W2384449405 @default.
- W2895827741 hasRelatedWork W25656500 @default.
- W2895827741 hasRelatedWork W2594402724 @default.
- W2895827741 hasRelatedWork W2896295279 @default.
- W2895827741 hasRelatedWork W2995418214 @default.
- W2895827741 hasRelatedWork W1000499199 @default.
- W2895827741 hasRelatedWork W2968792465 @default.
- W2895827741 hasRelatedWork W3042965305 @default.
- W2895827741 hasRelatedWork W3090114012 @default.
- W2895827741 hasRelatedWork W3181614907 @default.
- W2895827741 isParatext "false" @default.
- W2895827741 isRetracted "false" @default.
- W2895827741 magId "2895827741" @default.
- W2895827741 workType "article" @default.