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• W2895840796 abstract "Contact guidance or bidirectional migration along aligned fibers modulates many physiological and pathological processes such as wound healing and cancer invasion. Aligned 2D collagen fibrils epitaxially grown on mica substrates replicate many features of contact guidance seen in aligned 3D collagen fiber networks. However, these 2D collagen self-assembled substrates are difficult to image through, do not have known or tunable mechanical properties and cells degrade and mechanically detach collagen fibrils from the surface, leading to an inability to assess contact guidance over long times. Here, we describe the transfer of aligned collagen fibrils from mica substrates to three different functionalized target substrates: glass, polydimethylsiloxane (PDMS) and polyacrylamide (PA). Aligned collagen fibrils can be efficiently transferred to all three substrates. This transfer resulted in substrates that were to varying degrees resistant to cell-mediated collagen fibril deformation that resulted in detachment of the collagen fibril field, allowing for contact guidance to be observed over longer time periods. On these transferred substrates, cell speed is lowest on softer contact guidance cues for both MDA-MB-231 and MTLn3 cells. Intermediate stiffness resulted in the fastest migration. MTLn3 cell directionality was low on soft contact guidance cues, whereas MDA-MB-231 cell directionality marginally increased. It appears that the stiffness of the contact guidance cue regulates contact guidance differently between cell types. The development of this collagen fibril transfer method allows for the attachment of aligned collagen fibrils on substrates, particularly flexible substrates, that do not normally promote aligned collagen fibril growth, increasing the utility of this collagen self-assembly system for the fundamental examination of mechanical regulation of contact guidance." @default.
• W2895840796 created "2018-10-26" @default.
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• W2895840796 date "2018-01-01" @default.
• W2895840796 modified "2023-10-05" @default.
• W2895840796 title "Transfer of assembled collagen fibrils to flexible substrates for mechanically tunable contact guidance cues" @default.
• W2895840796 cites W1876890400 @default.
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• W2895840796 cites W1972052275 @default.
• W2895840796 cites W1973089587 @default.
• W2895840796 cites W1984100364 @default.
• W2895840796 cites W1984102813 @default.
• W2895840796 cites W1990391330 @default.
• W2895840796 cites W1994190469 @default.
• W2895840796 cites W1995256515 @default.
• W2895840796 cites W1996157869 @default.
• W2895840796 cites W1996841581 @default.
• W2895840796 cites W2004091053 @default.
• W2895840796 cites W2008303579 @default.
• W2895840796 cites W2009171739 @default.
• W2895840796 cites W2010251891 @default.
• W2895840796 cites W2016884409 @default.
• W2895840796 cites W2020425521 @default.
• W2895840796 cites W2037314810 @default.
• W2895840796 cites W2039312672 @default.
• W2895840796 cites W2042629182 @default.
• W2895840796 cites W2046986640 @default.
• W2895840796 cites W2054792338 @default.
• W2895840796 cites W2060613028 @default.
• W2895840796 cites W2062182733 @default.
• W2895840796 cites W2062390013 @default.
• W2895840796 cites W2063257532 @default.
• W2895840796 cites W2081965734 @default.
• W2895840796 cites W2082208320 @default.
• W2895840796 cites W2084933359 @default.
• W2895840796 cites W2086626063 @default.
• W2895840796 cites W2087456770 @default.
• W2895840796 cites W2099052265 @default.
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• W2895840796 cites W2104479723 @default.
• W2895840796 cites W2104848224 @default.
• W2895840796 cites W2105178180 @default.
• W2895840796 cites W2115124692 @default.
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• W2895840796 cites W2141738357 @default.
• W2895840796 cites W2142835646 @default.
• W2895840796 cites W2145675587 @default.
• W2895840796 cites W2148454908 @default.
• W2895840796 cites W2150991823 @default.
• W2895840796 cites W2161162374 @default.
• W2895840796 cites W2169873503 @default.
• W2895840796 cites W2286022789 @default.
• W2895840796 cites W2289120869 @default.
• W2895840796 cites W2306231350 @default.
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• W2895840796 cites W2543828283 @default.
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• W2895840796 cites W2565401689 @default.
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• W2895840796 cites W2607472835 @default.
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• W2895840796 doi "https://doi.org/10.1039/c8ib00127h" @default.
• W2895840796 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6267882" @default.
• W2895840796 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30320857" @default.
• W2895840796 hasPublicationYear "2018" @default.
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