FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895840982> ?p ?o ?g. }

• W2895840982 endingPage "562" @default.
• W2895840982 startingPage "557" @default.
• W2895840982 abstract "Intestinal fibrosis with stricture formation is a severe complication of Crohn's disease (CD). Though new therapeutic targets to enable the prevention or treatment of intestinal fibrosis are needed, markers of this condition and the basic mechanisms responsible have not been established. NADPH oxidase (NOX) 4 has already been reported to play a key role in models of fibrogenesis, including that of the lung. However, its importance in intestinal fibrogenesis remains unclear. In this study, we examined the role of NOX4 in collagen production by intestinal myofibroblasts stimulated with transforming growth factor (TGF)-β1. Using LmcMF cells, an intestinal subepithelial myofibroblast (ISEMF) line, we first examined the induction of collagen production by TGF-β1. Subsequently, we investigated the role of NOX4 in TGF-β1-induced collagen I production in these cells using SB525334 (an SMAD2/3 inhibitor), diphenyleneiodonium (an NOX inhibitor), and Nox4 small interfering RNA (siRNA). Production of collagen was assessed with Sirius red staining, and Nox4 expression was measured by quantitative real-time PCR. Reactive oxygen species (ROS) production was determined using DCFDA and fluorescent microscopy. We observed that TGF-β1 induced collagen production via NOX4 activation and ROS generation in LmcMF cells. Nox4 siRNA and inhibitors of TGF-β1 receptor and NOX significantly reduced TGF-β1-induced ROS and collagen production. Thus, in the present study, we revealed that collagen production in ISEMFs is induced via an NOX4-dependent pathway. This work supports a function for NOX4 in intestinal fibrogenesis and identifies it as a potential therapeutic target in recalcitrant fibrotic disorders of CD patients." @default.
• W2895840982 created "2018-10-26" @default.
• W2895840982 creator A5003444525 @default.
• W2895840982 creator A5003784214 @default.
• W2895840982 creator A5004262943 @default.
• W2895840982 creator A5012968391 @default.
• W2895840982 creator A5013887973 @default.
• W2895840982 creator A5018476721 @default.
• W2895840982 creator A5020259755 @default.
• W2895840982 creator A5024098161 @default.
• W2895840982 creator A5025761309 @default.
• W2895840982 creator A5026441567 @default.
• W2895840982 creator A5038459903 @default.
• W2895840982 creator A5042716966 @default.
• W2895840982 creator A5043149842 @default.
• W2895840982 creator A5043670524 @default.
• W2895840982 creator A5046070131 @default.
• W2895840982 creator A5053874033 @default.
• W2895840982 creator A5055620042 @default.
• W2895840982 creator A5059287690 @default.
• W2895840982 creator A5061869584 @default.
• W2895840982 creator A5066417029 @default.
• W2895840982 creator A5073846827 @default.
• W2895840982 creator A5076856789 @default.
• W2895840982 creator A5076985277 @default.
• W2895840982 creator A5082627499 @default.
• W2895840982 creator A5083248681 @default.
• W2895840982 creator A5084648926 @default.
• W2895840982 creator A5086052045 @default.
• W2895840982 date "2018-11-01" @default.
• W2895840982 modified "2023-09-27" @default.
• W2895840982 title "Transforming growth factor β1-induced collagen production in myofibroblasts is mediated by reactive oxygen species derived from NADPH oxidase 4" @default.
• W2895840982 cites W1947305094 @default.
• W2895840982 cites W1966672647 @default.
• W2895840982 cites W1973397356 @default.
• W2895840982 cites W1986241637 @default.
• W2895840982 cites W1995592152 @default.
• W2895840982 cites W1996139830 @default.
• W2895840982 cites W2025562467 @default.
• W2895840982 cites W2037478126 @default.
• W2895840982 cites W2063628403 @default.
• W2895840982 cites W2087467372 @default.
• W2895840982 cites W2115865518 @default.
• W2895840982 cites W2123242217 @default.
• W2895840982 cites W2130922818 @default.
• W2895840982 cites W2134215992 @default.
• W2895840982 cites W2142742192 @default.
• W2895840982 cites W2151088711 @default.
• W2895840982 cites W2168056382 @default.
• W2895840982 cites W2529401211 @default.
• W2895840982 cites W2540751197 @default.
• W2895840982 cites W2626035839 @default.
• W2895840982 cites W2753261645 @default.
• W2895840982 cites W2797560926 @default.
• W2895840982 doi "https://doi.org/10.1016/j.bbrc.2018.10.116" @default.
• W2895840982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30361098" @default.
• W2895840982 hasPublicationYear "2018" @default.
• W2895840982 type Work @default.
• W2895840982 sameAs 2895840982 @default.
• W2895840982 citedByCount "11" @default.
• W2895840982 countsByYear W28958409822020 @default.
• W2895840982 countsByYear W28958409822021 @default.
• W2895840982 countsByYear W28958409822022 @default.
• W2895840982 countsByYear W28958409822023 @default.
• W2895840982 crossrefType "journal-article" @default.
• W2895840982 hasAuthorship W2895840982A5003444525 @default.
• W2895840982 hasAuthorship W2895840982A5003784214 @default.
• W2895840982 hasAuthorship W2895840982A5004262943 @default.
• W2895840982 hasAuthorship W2895840982A5012968391 @default.
• W2895840982 hasAuthorship W2895840982A5013887973 @default.
• W2895840982 hasAuthorship W2895840982A5018476721 @default.
• W2895840982 hasAuthorship W2895840982A5020259755 @default.
• W2895840982 hasAuthorship W2895840982A5024098161 @default.
• W2895840982 hasAuthorship W2895840982A5025761309 @default.
• W2895840982 hasAuthorship W2895840982A5026441567 @default.
• W2895840982 hasAuthorship W2895840982A5038459903 @default.
• W2895840982 hasAuthorship W2895840982A5042716966 @default.
• W2895840982 hasAuthorship W2895840982A5043149842 @default.
• W2895840982 hasAuthorship W2895840982A5043670524 @default.
• W2895840982 hasAuthorship W2895840982A5046070131 @default.
• W2895840982 hasAuthorship W2895840982A5053874033 @default.
• W2895840982 hasAuthorship W2895840982A5055620042 @default.
• W2895840982 hasAuthorship W2895840982A5059287690 @default.
• W2895840982 hasAuthorship W2895840982A5061869584 @default.
• W2895840982 hasAuthorship W2895840982A5066417029 @default.
• W2895840982 hasAuthorship W2895840982A5073846827 @default.
• W2895840982 hasAuthorship W2895840982A5076856789 @default.
• W2895840982 hasAuthorship W2895840982A5076985277 @default.
• W2895840982 hasAuthorship W2895840982A5082627499 @default.
• W2895840982 hasAuthorship W2895840982A5083248681 @default.
• W2895840982 hasAuthorship W2895840982A5084648926 @default.
• W2895840982 hasAuthorship W2895840982A5086052045 @default.
• W2895840982 hasConcept C104317684 @default.
• W2895840982 hasConcept C118131993 @default.
• W2895840982 hasConcept C142724271 @default.
• W2895840982 hasConcept C153911025 @default.
• W2895840982 hasConcept C185592680 @default.
• W2895840982 hasConcept C207865475 @default.

• next