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- W2895849178 abstract "This thesis describes the research on two dye decolorizing peroxidases (DyP-type peroxidases). As the name of this enzyme family suggests, are these enzyme involved in the decolorization and degradation of in water soluble dyes. The textile and food industries are potential application fields of this enzyme family. The main goal of the research described in this thesis was to increase the knowledge on DyP-type peroxidases. Two bacterial enzymes were selected as model enzymes: TfuDyP from Thermobifida fusca and SviDyP from Saccharomonospora viridis. First, we studied on which compounds TfuDyP is active. This work showed that the enzyme is, among other compounds, active on phenols and a large variety of dyes, including dyes that were not studied before using DyP-type peroxidases (chapter 2). Furthermore, the reaction mechanism of the enzyme was studied (chapter 3). For the industrial applicability of enzymes an easy production method with a high enzyme yield is required. Chapter 4 describes a study aimed at optimizing the production of active TfuDyP. To be active, DyP-type peroxidases need to bind a heme cofactor. Results presented in chapter 4 provide crucial information on what can go wrong with the heme cofactor when a heme-containing protein is overexpressed (produced). Finally we fused SviDyP to four different oxidases (another type of enzyme) to perform two-step cascade reactions (chapter 5). With these fusion enzymes we were able to produce the taste enhancer divanilline and to detect the sugars xylitol and cellobiose in a solution." @default.
- W2895849178 created "2018-10-26" @default.
- W2895849178 creator A5028165541 @default.
- W2895849178 date "2018-01-01" @default.
- W2895849178 modified "2023-09-25" @default.
- W2895849178 title "Exploring the biochemical and biocatalytic properties of bacterial DyP-type peroxidases" @default.
- W2895849178 cites W1473670137 @default.
- W2895849178 cites W1518468234 @default.
- W2895849178 cites W1528593330 @default.
- W2895849178 cites W1536886974 @default.
- W2895849178 cites W1560288504 @default.
- W2895849178 cites W1589783447 @default.
- W2895849178 cites W1658725432 @default.
- W2895849178 cites W1864407288 @default.
- W2895849178 cites W1935158889 @default.
- W2895849178 cites W1964430082 @default.
- W2895849178 cites W1965114473 @default.
- W2895849178 cites W1965349074 @default.
- W2895849178 cites W1966719101 @default.
- W2895849178 cites W1967226836 @default.
- W2895849178 cites W1967821193 @default.
- W2895849178 cites W1970724810 @default.
- W2895849178 cites W1971435128 @default.
- W2895849178 cites W1973355402 @default.
- W2895849178 cites W1973440312 @default.
- W2895849178 cites W1973759673 @default.
- W2895849178 cites W1974073064 @default.
- W2895849178 cites W1977846540 @default.
- W2895849178 cites W1978520642 @default.
- W2895849178 cites W1981130018 @default.
- W2895849178 cites W1982859888 @default.
- W2895849178 cites W1985156329 @default.
- W2895849178 cites W1985308458 @default.
- W2895849178 cites W1987546499 @default.
- W2895849178 cites W1989884188 @default.
- W2895849178 cites W1990205765 @default.
- W2895849178 cites W1990392212 @default.
- W2895849178 cites W1990890819 @default.
- W2895849178 cites W1990970138 @default.
- W2895849178 cites W1991517216 @default.
- W2895849178 cites W1991774630 @default.
- W2895849178 cites W1992613244 @default.
- W2895849178 cites W1994827196 @default.
- W2895849178 cites W1994849220 @default.
- W2895849178 cites W1995529551 @default.
- W2895849178 cites W1996181006 @default.
- W2895849178 cites W1999800830 @default.
- W2895849178 cites W2000508647 @default.
- W2895849178 cites W2006826092 @default.
- W2895849178 cites W2008610282 @default.
- W2895849178 cites W2009181996 @default.
- W2895849178 cites W2009371233 @default.
- W2895849178 cites W2010763024 @default.
- W2895849178 cites W2011857157 @default.
- W2895849178 cites W2011929036 @default.
- W2895849178 cites W2012228951 @default.
- W2895849178 cites W2012321934 @default.
- W2895849178 cites W2012684695 @default.
- W2895849178 cites W2014944909 @default.
- W2895849178 cites W2016830482 @default.
- W2895849178 cites W2017662976 @default.
- W2895849178 cites W2017804400 @default.
- W2895849178 cites W2020194549 @default.
- W2895849178 cites W2023286233 @default.
- W2895849178 cites W2025017449 @default.
- W2895849178 cites W2025105469 @default.
- W2895849178 cites W2026155911 @default.
- W2895849178 cites W2027056249 @default.
- W2895849178 cites W2030393447 @default.
- W2895849178 cites W2031830549 @default.
- W2895849178 cites W2033481611 @default.
- W2895849178 cites W2033686736 @default.
- W2895849178 cites W2034729613 @default.
- W2895849178 cites W2036087350 @default.
- W2895849178 cites W2036686738 @default.
- W2895849178 cites W2038529067 @default.
- W2895849178 cites W2040883347 @default.
- W2895849178 cites W2041879090 @default.
- W2895849178 cites W2044091663 @default.
- W2895849178 cites W2044423696 @default.
- W2895849178 cites W2050271979 @default.
- W2895849178 cites W2051734899 @default.
- W2895849178 cites W2054579778 @default.
- W2895849178 cites W2059307992 @default.
- W2895849178 cites W2060323628 @default.
- W2895849178 cites W2060782384 @default.
- W2895849178 cites W2061939786 @default.
- W2895849178 cites W2063580702 @default.
- W2895849178 cites W2063968116 @default.
- W2895849178 cites W2064725174 @default.
- W2895849178 cites W2067356063 @default.
- W2895849178 cites W2067424042 @default.
- W2895849178 cites W2072520694 @default.
- W2895849178 cites W2074336931 @default.
- W2895849178 cites W2075331934 @default.
- W2895849178 cites W2075726306 @default.
- W2895849178 cites W2075990694 @default.
- W2895849178 cites W2079359179 @default.
- W2895849178 cites W2079914401 @default.
- W2895849178 cites W2080585129 @default.