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- W2895850171 abstract "Purpose To optimize the extraction process and to investigate antibacterial activity in vitro of ethanol extract of grape leaves Methods The extraction was extracted successively with ethanol in a soxhlet apparatus and the antibacterial activity was assessed by K B agar disc diffusion method The concentration of ethanol temperature time solid liquid ratio as factors for optimization Escherichia coli Staphylococcus aureus Candida albicans Bacillus subtilis as test organisms which used for antibacterial study and Staphylococcus aureus was choosed as the indicator organisms for the most obvious bacteriostatic effect The optimized extraction process was established by using single factor and orthogonal experiment which was guided by the diameter of inhibition zone Results Magnitude order of the effect of the four factors on extraction from grape leaves was ethanol concentration gt extraction temperature gt solid liquid ratio gt extraction time The best extraction condition as follows ethanol as extractant extraction temperature ratio of material to liquid extraction time h The order of bacteriostasis of ethanol extract of grape leaves was Staphylococcus aureus gt Escherichia coli gt Candida albicans gt Bacillus subtilis Conclusion The ethanol extract of grape leaves had significant antibacterial activity for Staphylococcus aureus and Escherichia coli especially Staphylococcus aureus while had no significant antibacterial activity for Bacillus subtilis The optimized extraction process was established by this paper can be used to extract anti bacterial substances and it has the advantages of fast speed easy operation and environmental protection" @default.
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- W2895850171 date "2018-04-10" @default.
- W2895850171 modified "2023-09-27" @default.
- W2895850171 title "Study the extraction process and antibacterial activity in vitro of ethanol extract of grape leaves" @default.
- W2895850171 doi "https://doi.org/10.15406/mojddt.2018.02.00029" @default.
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