FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895856039> ?p ?o ?g. }

• W2895856039 endingPage "1627" @default.
• W2895856039 startingPage "1617" @default.
• W2895856039 abstract "Carvedilol has been identified as a promising agent for the treatment of liver fibrosis. Meanwhile, autophagy and apoptosis have been reported to play key roles in the activation of hepatic stellate cells (HSCs), which can contribute to the progression of liver fibrosis. However, the effects of carvedilol on autophagy and apoptosis in HSCs remain unclear. Our study aimed to detect these effects and identify the underlying mechanisms by which carvedilol mediates HSC autophagy and apoptosis. For this purpose, the LX-2 cell line was used in this study, and the cells were exposed to various concentrations of carvedilol for specific times. First, we found that carvedilol increased autophagic marker levels, the number of GFP-LC3-containing puncta and LC3B-II levels in LX-2 cells. Interestingly, the addition of chloroquine (CQ) failed to enhance the effects on GFP-LC3 puncta and LC3B-II levels, and carvedilol treatment resulted in a significant increase in p62 protein levels. Moreover, carvedilol treatment led to the accumulation of yellow dots only in GFP-RFP-LC3-LX-2 cells, similar to the results following CQ treatment, indicating that carvedilol inhibited autophagic flux. Next, we found evidence that carvedilol inhibited autophagic flux by increasing lysosomal pH and not by impairing the fusion of autophagosomes with lysosomes. Moreover, carvedilol substantially reduced the viability of LX-2 cells and noticeably induced cell apoptosis, as observed by flow cytometry. In addition, increased levels of cleaved caspase-3, cleaved caspase-8 and cleaved PARP, increased Bax activity and decreased Bcl-2 expression were detected in LX-2 cells. Finally, the carvedilol treatment inhibited autophagy and subsequently induced apoptosis in vitro. In conclusion, carvedilol suppresses autophagy and promotes apoptosis in HSCs and the late-stage inhibition of autophagy preceded the induction of apoptosis." @default.
• W2895856039 created "2018-10-26" @default.
• W2895856039 creator A5018206429 @default.
• W2895856039 creator A5035520790 @default.
• W2895856039 creator A5064566639 @default.
• W2895856039 creator A5067168494 @default.
• W2895856039 creator A5074451157 @default.
• W2895856039 creator A5085056832 @default.
• W2895856039 date "2018-12-01" @default.
• W2895856039 modified "2023-10-16" @default.
• W2895856039 title "Carvedilol attenuates liver fibrosis by suppressing autophagy and promoting apoptosis in hepatic stellate cells" @default.
• W2895856039 cites W1982513324 @default.
• W2895856039 cites W1997005484 @default.
• W2895856039 cites W2001247235 @default.
• W2895856039 cites W2005270729 @default.
• W2895856039 cites W2014918593 @default.
• W2895856039 cites W2017656880 @default.
• W2895856039 cites W2017784625 @default.
• W2895856039 cites W2026873116 @default.
• W2895856039 cites W2036994468 @default.
• W2895856039 cites W2057992903 @default.
• W2895856039 cites W2059655994 @default.
• W2895856039 cites W2069457144 @default.
• W2895856039 cites W2090251406 @default.
• W2895856039 cites W2098649404 @default.
• W2895856039 cites W2104193042 @default.
• W2895856039 cites W2106255061 @default.
• W2895856039 cites W2111288662 @default.
• W2895856039 cites W2115268409 @default.
• W2895856039 cites W2142988890 @default.
• W2895856039 cites W2145822917 @default.
• W2895856039 cites W2146558650 @default.
• W2895856039 cites W2147934487 @default.
• W2895856039 cites W2159754384 @default.
• W2895856039 cites W2164634147 @default.
• W2895856039 cites W2167248390 @default.
• W2895856039 cites W2283924758 @default.
• W2895856039 cites W2342186404 @default.
• W2895856039 cites W2409327016 @default.
• W2895856039 cites W2545247739 @default.
• W2895856039 cites W2564624265 @default.
• W2895856039 cites W2567682468 @default.
• W2895856039 cites W2588120710 @default.
• W2895856039 cites W2605260329 @default.
• W2895856039 cites W2624796717 @default.
• W2895856039 cites W2787854599 @default.
• W2895856039 cites W2807682250 @default.
• W2895856039 cites W302404286 @default.
• W2895856039 doi "https://doi.org/10.1016/j.biopha.2018.10.005" @default.
• W2895856039 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30372864" @default.
• W2895856039 hasPublicationYear "2018" @default.
• W2895856039 type Work @default.
• W2895856039 sameAs 2895856039 @default.
• W2895856039 citedByCount "69" @default.
• W2895856039 countsByYear W28958560392019 @default.
• W2895856039 countsByYear W28958560392020 @default.
• W2895856039 countsByYear W28958560392021 @default.
• W2895856039 countsByYear W28958560392022 @default.
• W2895856039 countsByYear W28958560392023 @default.
• W2895856039 crossrefType "journal-article" @default.
• W2895856039 hasAuthorship W2895856039A5018206429 @default.
• W2895856039 hasAuthorship W2895856039A5035520790 @default.
• W2895856039 hasAuthorship W2895856039A5064566639 @default.
• W2895856039 hasAuthorship W2895856039A5067168494 @default.
• W2895856039 hasAuthorship W2895856039A5074451157 @default.
• W2895856039 hasAuthorship W2895856039A5085056832 @default.
• W2895856039 hasBestOaLocation W28958560391 @default.
• W2895856039 hasConcept C126322002 @default.
• W2895856039 hasConcept C134018914 @default.
• W2895856039 hasConcept C153911025 @default.
• W2895856039 hasConcept C176891718 @default.
• W2895856039 hasConcept C185592680 @default.
• W2895856039 hasConcept C190283241 @default.
• W2895856039 hasConcept C203522944 @default.
• W2895856039 hasConcept C2778198053 @default.
• W2895856039 hasConcept C2780559512 @default.
• W2895856039 hasConcept C2780591200 @default.
• W2895856039 hasConcept C502942594 @default.
• W2895856039 hasConcept C53227056 @default.
• W2895856039 hasConcept C553184892 @default.
• W2895856039 hasConcept C55493867 @default.
• W2895856039 hasConcept C71924100 @default.
• W2895856039 hasConcept C86803240 @default.
• W2895856039 hasConcept C95444343 @default.
• W2895856039 hasConcept C98274493 @default.
• W2895856039 hasConceptScore W2895856039C126322002 @default.
• W2895856039 hasConceptScore W2895856039C134018914 @default.
• W2895856039 hasConceptScore W2895856039C153911025 @default.
• W2895856039 hasConceptScore W2895856039C176891718 @default.
• W2895856039 hasConceptScore W2895856039C185592680 @default.
• W2895856039 hasConceptScore W2895856039C190283241 @default.
• W2895856039 hasConceptScore W2895856039C203522944 @default.
• W2895856039 hasConceptScore W2895856039C2778198053 @default.
• W2895856039 hasConceptScore W2895856039C2780559512 @default.
• W2895856039 hasConceptScore W2895856039C2780591200 @default.
• W2895856039 hasConceptScore W2895856039C502942594 @default.
• W2895856039 hasConceptScore W2895856039C53227056 @default.
• W2895856039 hasConceptScore W2895856039C553184892 @default.

• next