FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895880890> ?p ?o ?g. }

• W2895880890 endingPage "131" @default.
• W2895880890 startingPage "126" @default.
• W2895880890 abstract "CHRFAM7A is a uniquely-human gene that encodes a human-specific variant of the alpha-7 nicotinic acetylcholine receptor (α7nAchR). While the homopentameric α7nAChR consists of 5 equal subunits, previous studies demonstrated that CHRFAM7A expression disrupts the formation of α7nAChR homopentamers. Here we use a rat neuronal cell line expressing CHRFAM7A and a transgenic mouse expressing CHRFAM7A to define the alpha-bungarotoxin (α-BTX) binding in vitro and in vivo. Rat PC12 cells were stably transfected with human CHRFAM7A. α-BTX, a protein that irreversibly binds the α7nAchR, was utilized to assess the capacity for CHRFAM7A to interfere with α 7AchR subunits using immunohistochemistry and flow cytometry. To evaluate the effects of CHRFAM7A on α7nAchR at the neuromuscular junction in vivo, transgenic mice were engineered to express the uniquely human gene CHRFAM7A under the control of the EF1-α promoter. Using this model, muscle was harvested and CHRFAM7A and CHRNA7 gene expression evaluated by PCR. Binding of α-BTX to the α7nAchR in muscle was compared in sibling-matched wild-type C57 mice by immunostaining the neuromuscular junction using α-BTX and neurofilament antibodies. Expression of CHRFAM7A in transfected, but not vector cells, was confirmed by PCR and by immunoblotting using an antibody we raised to a peptide sequence unique to CHRFAM7A. CHRFAM7A decreased α-BTX binding as detected by immunohistochemistry and flow cytometry. In vivo, α-BTX co-stained with neurofilament at the neuromuscular junction in wild-type mice, however, α-BTX staining was decreased at the neuromuscular junction of CHRFAM7A transgenic mice. CHRFAM7A expression interferes with the binding of α7nAchR to α-BTX. Understanding the contribution of this uniquely human gene to human disease will be important in the identification of potential therapeutic targets." @default.
• W2895880890 created "2018-10-26" @default.
• W2895880890 creator A5001537127 @default.
• W2895880890 creator A5009934199 @default.
• W2895880890 creator A5024786945 @default.
• W2895880890 creator A5037880923 @default.
• W2895880890 creator A5041965368 @default.
• W2895880890 creator A5065261693 @default.
• W2895880890 date "2019-01-01" @default.
• W2895880890 modified "2023-10-15" @default.
• W2895880890 title "CHRFAM7A alters binding to the neuronal alpha-7 nicotinic acetylcholine receptor" @default.
• W2895880890 cites W1901500258 @default.
• W2895880890 cites W1971784543 @default.
• W2895880890 cites W1987695713 @default.
• W2895880890 cites W1987902393 @default.
• W2895880890 cites W1990922607 @default.
• W2895880890 cites W1996121363 @default.
• W2895880890 cites W2003298566 @default.
• W2895880890 cites W2023236906 @default.
• W2895880890 cites W2054030400 @default.
• W2895880890 cites W2072807117 @default.
• W2895880890 cites W2074617527 @default.
• W2895880890 cites W2091478089 @default.
• W2895880890 cites W2100888675 @default.
• W2895880890 cites W2115614798 @default.
• W2895880890 cites W2127620928 @default.
• W2895880890 cites W2150473059 @default.
• W2895880890 cites W2163106343 @default.
• W2895880890 cites W2185510808 @default.
• W2895880890 cites W2238515597 @default.
• W2895880890 cites W2804309680 @default.
• W2895880890 cites W2883258870 @default.
• W2895880890 cites W324111633 @default.
• W2895880890 doi "https://doi.org/10.1016/j.neulet.2018.10.010" @default.
• W2895880890 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6320298" @default.
• W2895880890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30308236" @default.
• W2895880890 hasPublicationYear "2019" @default.
• W2895880890 type Work @default.
• W2895880890 sameAs 2895880890 @default.
• W2895880890 citedByCount "14" @default.
• W2895880890 countsByYear W28958808902019 @default.
• W2895880890 countsByYear W28958808902020 @default.
• W2895880890 countsByYear W28958808902021 @default.
• W2895880890 countsByYear W28958808902022 @default.
• W2895880890 countsByYear W28958808902023 @default.
• W2895880890 crossrefType "journal-article" @default.
• W2895880890 hasAuthorship W2895880890A5001537127 @default.
• W2895880890 hasAuthorship W2895880890A5009934199 @default.
• W2895880890 hasAuthorship W2895880890A5024786945 @default.
• W2895880890 hasAuthorship W2895880890A5037880923 @default.
• W2895880890 hasAuthorship W2895880890A5041965368 @default.
• W2895880890 hasAuthorship W2895880890A5065261693 @default.
• W2895880890 hasBestOaLocation W28958808902 @default.
• W2895880890 hasConcept C102230213 @default.
• W2895880890 hasConcept C104317684 @default.
• W2895880890 hasConcept C141035611 @default.
• W2895880890 hasConcept C153911025 @default.
• W2895880890 hasConcept C157207234 @default.
• W2895880890 hasConcept C169760540 @default.
• W2895880890 hasConcept C170493617 @default.
• W2895880890 hasConcept C203014093 @default.
• W2895880890 hasConcept C204232928 @default.
• W2895880890 hasConcept C2777240379 @default.
• W2895880890 hasConcept C2779570518 @default.
• W2895880890 hasConcept C4224716 @default.
• W2895880890 hasConcept C54009773 @default.
• W2895880890 hasConcept C54355233 @default.
• W2895880890 hasConcept C55493867 @default.
• W2895880890 hasConcept C80161118 @default.
• W2895880890 hasConcept C81885089 @default.
• W2895880890 hasConcept C86803240 @default.
• W2895880890 hasConcept C95444343 @default.
• W2895880890 hasConceptScore W2895880890C102230213 @default.
• W2895880890 hasConceptScore W2895880890C104317684 @default.
• W2895880890 hasConceptScore W2895880890C141035611 @default.
• W2895880890 hasConceptScore W2895880890C153911025 @default.
• W2895880890 hasConceptScore W2895880890C157207234 @default.
• W2895880890 hasConceptScore W2895880890C169760540 @default.
• W2895880890 hasConceptScore W2895880890C170493617 @default.
• W2895880890 hasConceptScore W2895880890C203014093 @default.
• W2895880890 hasConceptScore W2895880890C204232928 @default.
• W2895880890 hasConceptScore W2895880890C2777240379 @default.
• W2895880890 hasConceptScore W2895880890C2779570518 @default.
• W2895880890 hasConceptScore W2895880890C4224716 @default.
• W2895880890 hasConceptScore W2895880890C54009773 @default.
• W2895880890 hasConceptScore W2895880890C54355233 @default.
• W2895880890 hasConceptScore W2895880890C55493867 @default.
• W2895880890 hasConceptScore W2895880890C80161118 @default.
• W2895880890 hasConceptScore W2895880890C81885089 @default.
• W2895880890 hasConceptScore W2895880890C86803240 @default.
• W2895880890 hasConceptScore W2895880890C95444343 @default.
• W2895880890 hasFunder F4320332161 @default.
• W2895880890 hasLocation W28958808901 @default.
• W2895880890 hasLocation W28958808902 @default.
• W2895880890 hasLocation W28958808903 @default.
• W2895880890 hasLocation W28958808904 @default.
• W2895880890 hasLocation W28958808905 @default.
• W2895880890 hasOpenAccess W2895880890 @default.

• next