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- W2895888195 abstract "• A catalog of confirmed hot spots in the CHO genome is compiled. • Emerging strategies for more efficient nuclease-mediated SSI are highlighted. • Progress toward multi-site and accumulative SSI for controlled gene copy number is discussed. Chinese hamster ovary (CHO) cells are widely used for the production of therapeutic proteins. Customarily, CHO production cell lines are established through random integration, which requires laborious screening of many clones to isolate suitable producers. In contrast, site-specific integration (SSI) accelerates cell line development by targeting integration of transgenes to pre-validated genomic loci capable of supporting high and stable expression. To date, a relatively small number of these so called ‘hot spots’ have been identified, mainly through empirical methods. Nevertheless, nuclease-mediated and recombinase-mediated SSI have revolutionized cell line engineering by enabling rational and reproducible transgene targeting." @default.
- W2895888195 created "2018-10-26" @default.
- W2895888195 creator A5050864999 @default.
- W2895888195 creator A5060404458 @default.
- W2895888195 date "2018-12-01" @default.
- W2895888195 modified "2023-10-11" @default.
- W2895888195 title "Site-specific integration ushers in a new era of precise CHO cell line engineering" @default.
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- W2895888195 doi "https://doi.org/10.1016/j.coche.2018.09.011" @default.
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