Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895895706> ?p ?o ?g. }
- W2895895706 endingPage "5464" @default.
- W2895895706 startingPage "5448" @default.
- W2895895706 abstract "The pathogenesis of ischemic diseases remains unclear. Here we demonstrate the induction of microRNA-668 (miR-668) in ischemic acute kidney injury (AKI) in human patients, mice, and renal tubular cells. The induction was HIF-1 dependent, as HIF-1 deficiency in cells and kidney proximal tubules attenuated miR-668 expression. We further identified a functional HIF-1 binding site in the miR-668 gene promoter. Anti–miR-668 increased apoptosis in renal tubular cells and enhanced ischemic AKI in mice, whereas miR-668 mimic was protective. Mechanistically, anti–miR-668 induced mitochondrial fragmentation, whereas miR-668 blocked mitochondrial fragmentation during hypoxia. We analyzed miR-668 target genes through immunoprecipitation of microRNA-induced silencing complexes followed by RNA deep sequencing and identified 124 protein-coding genes as likely targets of miR-668. Among these genes, only mitochondrial protein 18 kDa (MTP18) has been implicated in mitochondrial dynamics. In renal cells and mouse kidneys, miR-668 mimic suppressed MTP18, whereas anti–miR-668 increased MTP18 expression. Luciferase microRNA target reporter assay further verified MTP18 as a direct target of miR-668. In renal tubular cells, knockdown of MTP18 suppressed mitochondrial fragmentation and apoptosis. Together, the results suggest that miR-668 is induced via HIF-1 in ischemic AKI and that, upon induction, miR-668 represses MTP18 to preserve mitochondrial dynamics for renal tubular cell survival and kidney protection." @default.
- W2895895706 created "2018-10-26" @default.
- W2895895706 creator A5000748289 @default.
- W2895895706 creator A5001122836 @default.
- W2895895706 creator A5002687021 @default.
- W2895895706 creator A5010689362 @default.
- W2895895706 creator A5012613471 @default.
- W2895895706 creator A5032811968 @default.
- W2895895706 creator A5033080900 @default.
- W2895895706 creator A5046465689 @default.
- W2895895706 creator A5050155862 @default.
- W2895895706 creator A5056579154 @default.
- W2895895706 creator A5058258341 @default.
- W2895895706 creator A5071419704 @default.
- W2895895706 creator A5081888342 @default.
- W2895895706 date "2018-11-12" @default.
- W2895895706 modified "2023-10-10" @default.
- W2895895706 title "MicroRNA-668 represses MTP18 to preserve mitochondrial dynamics in ischemic acute kidney injury" @default.
- W2895895706 cites W1660232022 @default.
- W2895895706 cites W1963874479 @default.
- W2895895706 cites W1965191283 @default.
- W2895895706 cites W1965518526 @default.
- W2895895706 cites W1969475086 @default.
- W2895895706 cites W1971228736 @default.
- W2895895706 cites W1971799714 @default.
- W2895895706 cites W1973966118 @default.
- W2895895706 cites W1975366235 @default.
- W2895895706 cites W1978134915 @default.
- W2895895706 cites W1989026904 @default.
- W2895895706 cites W1996846107 @default.
- W2895895706 cites W2002321863 @default.
- W2895895706 cites W2005673220 @default.
- W2895895706 cites W2008971670 @default.
- W2895895706 cites W2010369463 @default.
- W2895895706 cites W2020871623 @default.
- W2895895706 cites W2028933700 @default.
- W2895895706 cites W2030294672 @default.
- W2895895706 cites W2044993806 @default.
- W2895895706 cites W2046011845 @default.
- W2895895706 cites W2067067767 @default.
- W2895895706 cites W2067463198 @default.
- W2895895706 cites W2068362864 @default.
- W2895895706 cites W2073625832 @default.
- W2895895706 cites W2076542737 @default.
- W2895895706 cites W2085335902 @default.
- W2895895706 cites W2085367705 @default.
- W2895895706 cites W2099428640 @default.
- W2895895706 cites W2103313018 @default.
- W2895895706 cites W2103490409 @default.
- W2895895706 cites W2103950684 @default.
- W2895895706 cites W2109851203 @default.
- W2895895706 cites W2116799486 @default.
- W2895895706 cites W2131176327 @default.
- W2895895706 cites W2131897198 @default.
- W2895895706 cites W2133380348 @default.
- W2895895706 cites W2133497102 @default.
- W2895895706 cites W2134943860 @default.
- W2895895706 cites W2135705997 @default.
- W2895895706 cites W2137465719 @default.
- W2895895706 cites W2146942628 @default.
- W2895895706 cites W2147375079 @default.
- W2895895706 cites W2154258399 @default.
- W2895895706 cites W2155886511 @default.
- W2895895706 cites W2155978870 @default.
- W2895895706 cites W2157334943 @default.
- W2895895706 cites W2163545533 @default.
- W2895895706 cites W2164793313 @default.
- W2895895706 cites W2169378494 @default.
- W2895895706 cites W2203566365 @default.
- W2895895706 cites W2297008280 @default.
- W2895895706 cites W2323381797 @default.
- W2895895706 cites W2371948111 @default.
- W2895895706 cites W2481332484 @default.
- W2895895706 cites W2519391457 @default.
- W2895895706 cites W2519716821 @default.
- W2895895706 cites W2528461366 @default.
- W2895895706 cites W2554944956 @default.
- W2895895706 cites W2580423083 @default.
- W2895895706 cites W2581025349 @default.
- W2895895706 cites W2607212548 @default.
- W2895895706 cites W2609383274 @default.
- W2895895706 cites W2611455804 @default.
- W2895895706 cites W2612061536 @default.
- W2895895706 cites W2750514629 @default.
- W2895895706 cites W2765100145 @default.
- W2895895706 cites W2766902151 @default.
- W2895895706 doi "https://doi.org/10.1172/jci121859" @default.
- W2895895706 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6264638" @default.
- W2895895706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30325740" @default.
- W2895895706 hasPublicationYear "2018" @default.
- W2895895706 type Work @default.
- W2895895706 sameAs 2895895706 @default.
- W2895895706 citedByCount "84" @default.
- W2895895706 countsByYear W28958957062018 @default.
- W2895895706 countsByYear W28958957062019 @default.
- W2895895706 countsByYear W28958957062020 @default.
- W2895895706 countsByYear W28958957062021 @default.
- W2895895706 countsByYear W28958957062022 @default.