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• W2895899729 endingPage "18840" @default.
• W2895899729 startingPage "18828" @default.
• W2895899729 abstract "For most retroviruses, including HIV-1, binding of the Gag polyprotein to the plasma membrane (PM) is mediated by interactions between Gag's N-terminal myristoylated matrix (MA) domain and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) in the PM. The Gag protein of avian sarcoma virus (ASV) lacks the N-myristoylation signal but contains structural domains having functions similar to those of HIV-1 Gag. The molecular mechanism by which ASV Gag binds to the PM is incompletely understood. Here, we employed NMR techniques to elucidate the molecular determinants of the membrane-binding domain of ASV MA (MA87) to lipids and liposomes. We report that MA87 binds to the polar head of phosphoinositides such as PI(4,5)P2. We found that MA87 binding to inositol phosphates (IPs) is significantly enhanced by increasing the number of phosphate groups, indicating that the MA87–IP binding is governed by charge–charge interactions. Using a sensitive NMR-based liposome-binding assay, we show that binding of MA87 to liposomes is enhanced by incorporation of PI(4,5)P2 and phosphatidylserine. We also show that membrane binding is mediated by a basic surface formed by Lys-6, Lys-13, Lys-23, and Lys-24. Substitution of these residues to glutamate abolished binding of MA87 to both IPs and liposomes. In an accompanying paper, we further report that mutation of these lysine residues diminishes Gag assembly on the PM and inhibits ASV particle release. These findings provide a molecular basis for ASV Gag binding to the inner leaflet of the PM and advance our understanding of the basic mechanisms of retroviral assembly." @default.
• W2895899729 created "2018-10-26" @default.
• W2895899729 creator A5002498667 @default.
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• W2895899729 creator A5068183892 @default.
• W2895899729 date "2018-12-01" @default.
• W2895899729 modified "2023-10-12" @default.
• W2895899729 title "Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assembly" @default.
• W2895899729 cites W1608246545 @default.
• W2895899729 cites W1823429634 @default.
• W2895899729 cites W1848132941 @default.
• W2895899729 cites W1852463974 @default.
• W2895899729 cites W1950488331 @default.
• W2895899729 cites W1965498370 @default.
• W2895899729 cites W1967986789 @default.
• W2895899729 cites W1971234593 @default.
• W2895899729 cites W1977860515 @default.
• W2895899729 cites W1982692592 @default.
• W2895899729 cites W1982997055 @default.
• W2895899729 cites W1991586131 @default.
• W2895899729 cites W1996779413 @default.
• W2895899729 cites W1997279728 @default.
• W2895899729 cites W1999551487 @default.
• W2895899729 cites W2000569926 @default.
• W2895899729 cites W2011313782 @default.
• W2895899729 cites W2015140940 @default.
• W2895899729 cites W2017522157 @default.
• W2895899729 cites W2020867902 @default.
• W2895899729 cites W2023916724 @default.
• W2895899729 cites W2025472947 @default.
• W2895899729 cites W2028046413 @default.
• W2895899729 cites W2029582401 @default.
• W2895899729 cites W2029667189 @default.
• W2895899729 cites W2030971064 @default.
• W2895899729 cites W2031298430 @default.
• W2895899729 cites W2034684407 @default.
• W2895899729 cites W2035694643 @default.
• W2895899729 cites W2045683699 @default.
• W2895899729 cites W2046832161 @default.
• W2895899729 cites W2047399603 @default.
• W2895899729 cites W2052933818 @default.
• W2895899729 cites W2055921223 @default.
• W2895899729 cites W2057712924 @default.
• W2895899729 cites W2057723413 @default.
• W2895899729 cites W2063613016 @default.
• W2895899729 cites W2072683386 @default.
• W2895899729 cites W2077057101 @default.
• W2895899729 cites W2082176639 @default.
• W2895899729 cites W2085035388 @default.
• W2895899729 cites W2093074309 @default.
• W2895899729 cites W2094692623 @default.
• W2895899729 cites W2096621401 @default.
• W2895899729 cites W2102965972 @default.
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• W2895899729 cites W2124493183 @default.
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• W2895899729 cites W2139457276 @default.
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• W2895899729 cites W2153363820 @default.
• W2895899729 cites W2153983456 @default.
• W2895899729 cites W2154140185 @default.
• W2895899729 cites W2156651686 @default.
• W2895899729 cites W2159148283 @default.
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• W2895899729 cites W2167154513 @default.
• W2895899729 cites W2169707043 @default.
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• W2895899729 cites W2767856283 @default.
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• W2895899729 doi "https://doi.org/10.1074/jbc.ra118.003944" @default.
• W2895899729 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6295720" @default.
• W2895899729 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30309983" @default.
• W2895899729 hasPublicationYear "2018" @default.
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