Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895900785> ?p ?o ?g. }
- W2895900785 abstract "Cell-surface N-glycans play important roles in both inter- and intracellular processes, including cell adhesion and development, cell recognition, as well as cancer development and metastasis; detailed structural characterization of these N-glycans is thus paramount. Here we report our comparative N-glycomics study of cell-surface N-glycans of the hepatocellular carcinoma (HCC) HepG2 cells vs the normal liver LO2 cells. With sequential trypsin digestion of proteins, C18 depletion of peptides without glycosylation, PNGase F digestion of N-glycopeptides, PGC enrichment of N-glycans, CH3I permethylation of the enriched N-glycans, cell-surface N-glycomes of the HepG2 and LO2 cells were analyzed using C18-RPLC–MS/MS (HCD). With spectrum-level FDR no bigger than 1%, 351 and 310 N-glycans were identified for HepG2 and LO2, respectively, with comprehensive structural information (not only monosaccharide composition, but also sequence and linkage) by N-glycan database search engine GlySeeker. The percentage of hybrid N-glycans with tetra-antennary structures was substantially increased in the HepG2 cells. This comprehensive discovery study of differentially expressed cell-surface N-glycans in HepG2 vs LO2 serves as a solid reference for future validation study of glycosylation markers in HCC." @default.
- W2895900785 created "2018-10-26" @default.
- W2895900785 creator A5039887185 @default.
- W2895900785 creator A5042231897 @default.
- W2895900785 creator A5059424766 @default.
- W2895900785 date "2018-10-29" @default.
- W2895900785 modified "2023-10-02" @default.
- W2895900785 title "Comparative Glycomics Study of Cell-Surface N-Glycomes of HepG2 versus LO2 Cell Lines" @default.
- W2895900785 cites W1496805086 @default.
- W2895900785 cites W1886541649 @default.
- W2895900785 cites W1968995658 @default.
- W2895900785 cites W1977723151 @default.
- W2895900785 cites W1983161997 @default.
- W2895900785 cites W1994441900 @default.
- W2895900785 cites W1995215898 @default.
- W2895900785 cites W2009494587 @default.
- W2895900785 cites W2022717671 @default.
- W2895900785 cites W2022754675 @default.
- W2895900785 cites W2038703083 @default.
- W2895900785 cites W2052644289 @default.
- W2895900785 cites W2056767182 @default.
- W2895900785 cites W2064961433 @default.
- W2895900785 cites W2068339549 @default.
- W2895900785 cites W2070442681 @default.
- W2895900785 cites W2071994095 @default.
- W2895900785 cites W2072079749 @default.
- W2895900785 cites W2142398112 @default.
- W2895900785 cites W2156381487 @default.
- W2895900785 cites W2160197060 @default.
- W2895900785 cites W2161909728 @default.
- W2895900785 cites W2168824556 @default.
- W2895900785 cites W2171192777 @default.
- W2895900785 cites W2315438426 @default.
- W2895900785 cites W2325402346 @default.
- W2895900785 cites W2328176321 @default.
- W2895900785 cites W2409983941 @default.
- W2895900785 cites W2410718960 @default.
- W2895900785 cites W2437541722 @default.
- W2895900785 cites W2460485266 @default.
- W2895900785 cites W2462214341 @default.
- W2895900785 cites W2495267426 @default.
- W2895900785 cites W2506249512 @default.
- W2895900785 cites W2512870575 @default.
- W2895900785 cites W2531066839 @default.
- W2895900785 cites W2588101825 @default.
- W2895900785 cites W2597717267 @default.
- W2895900785 cites W2604820238 @default.
- W2895900785 cites W2721015324 @default.
- W2895900785 cites W2728426806 @default.
- W2895900785 cites W2746244293 @default.
- W2895900785 cites W2763257913 @default.
- W2895900785 cites W2766154556 @default.
- W2895900785 cites W2800094925 @default.
- W2895900785 doi "https://doi.org/10.1021/acs.jproteome.8b00655" @default.
- W2895900785 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30343578" @default.
- W2895900785 hasPublicationYear "2018" @default.
- W2895900785 type Work @default.
- W2895900785 sameAs 2895900785 @default.
- W2895900785 citedByCount "4" @default.
- W2895900785 countsByYear W28959007852021 @default.
- W2895900785 countsByYear W28959007852022 @default.
- W2895900785 countsByYear W28959007852023 @default.
- W2895900785 crossrefType "journal-article" @default.
- W2895900785 hasAuthorship W2895900785A5039887185 @default.
- W2895900785 hasAuthorship W2895900785A5042231897 @default.
- W2895900785 hasAuthorship W2895900785A5059424766 @default.
- W2895900785 hasConcept C106686826 @default.
- W2895900785 hasConcept C108625454 @default.
- W2895900785 hasConcept C1491633281 @default.
- W2895900785 hasConcept C185592680 @default.
- W2895900785 hasConcept C206212055 @default.
- W2895900785 hasConcept C2776817266 @default.
- W2895900785 hasConcept C2777313579 @default.
- W2895900785 hasConcept C2780654058 @default.
- W2895900785 hasConcept C30324644 @default.
- W2895900785 hasConcept C55493867 @default.
- W2895900785 hasConcept C86803240 @default.
- W2895900785 hasConceptScore W2895900785C106686826 @default.
- W2895900785 hasConceptScore W2895900785C108625454 @default.
- W2895900785 hasConceptScore W2895900785C1491633281 @default.
- W2895900785 hasConceptScore W2895900785C185592680 @default.
- W2895900785 hasConceptScore W2895900785C206212055 @default.
- W2895900785 hasConceptScore W2895900785C2776817266 @default.
- W2895900785 hasConceptScore W2895900785C2777313579 @default.
- W2895900785 hasConceptScore W2895900785C2780654058 @default.
- W2895900785 hasConceptScore W2895900785C30324644 @default.
- W2895900785 hasConceptScore W2895900785C55493867 @default.
- W2895900785 hasConceptScore W2895900785C86803240 @default.
- W2895900785 hasFunder F4320321001 @default.
- W2895900785 hasFunder F4320321885 @default.
- W2895900785 hasLocation W28959007851 @default.
- W2895900785 hasLocation W28959007852 @default.
- W2895900785 hasOpenAccess W2895900785 @default.
- W2895900785 hasPrimaryLocation W28959007851 @default.
- W2895900785 hasRelatedWork W1975019028 @default.
- W2895900785 hasRelatedWork W2014436828 @default.
- W2895900785 hasRelatedWork W2071911878 @default.
- W2895900785 hasRelatedWork W2124625378 @default.
- W2895900785 hasRelatedWork W2312304310 @default.
- W2895900785 hasRelatedWork W2747326776 @default.