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• W2895918907 abstract "Abstract BACKGROUND Next-generation sequencing (NGS) is revolutionizing a variety of molecular biology fields including bioforensics, biosurveillance, and infectious disease diagnostics. For pathogen detection, the ability to sequence all nucleic acids in a sample allows near limitless multiplexability, free from a priori knowledge regarding an etiologic agent as is typically required for targeted molecular assays such as real-time PCR. Furthermore, sequencing capabilities can generate in depth genomic information, allowing detailed molecular epidemiological studies and bioforensics analysis, which is critical for source agent identification in a biothreat outbreak. However, lack of analytical specificity, inherent to NGS, presents challenges for regulated applications such as clinical diagnostics and molecular attribution. CONTENT Here, we discuss NGS applications in the context of preparedness and biothreat readiness. Specifically, we investigate current and future applications of NGS technologies to affect the fields of biosurveillance, bioforensics, and clinical diagnostics with specific focus on biodefense. SUMMARY Overall, there are many advantages to the implementation of NGS for preparedness and readiness against biowarfare agents, from forensics to diagnostics. However, appropriate caveats must be associated with any technology. This includes NGS. While NGS is not the panacea replacing all molecular techniques, it will greatly enhance the ability to detect, characterize, and diagnose biowarfare agents, thus providing an excellent addition to the biodefense toolbox of biosurveillance, bioforensics, and biothreat diagnosis." @default.
• W2895918907 created "2018-10-26" @default.
• W2895918907 creator A5011142331 @default.
• W2895918907 creator A5038394573 @default.
• W2895918907 creator A5053139001 @default.
• W2895918907 creator A5087015233 @default.
• W2895918907 date "2019-03-01" @default.
• W2895918907 modified "2023-10-04" @default.
• W2895918907 title "Next-Generation Sequencing for Biodefense: Biothreat Detection, Forensics, and the Clinic" @default.
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• W2895918907 doi "https://doi.org/10.1373/clinchem.2016.266536" @default.
• W2895918907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30352865" @default.
• W2895918907 hasPublicationYear "2019" @default.
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