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- W2895925917 abstract "Initially, 60 primers were screened among 32 genotypes of little millet (Panicum sumatrense), from which 36 primers were selected on the basis of a sharp and clear banding pattern for final random amplified polymorphic DNA (RAPD) analysis. The PCR reaction was carried out using a single decamer primer at a time. In total, 175 RAPD marker loci were amplified of which 155 were polymorphic (88.58%). The band size of amplified markers was in the range 100–1900 bp. Ten bands were the maximum scored (for primer OPH-19) whereas two bands were the minimum scored (for primer OPC-13). On average, 4.86 bands per primer and 4.31 polymorphic bands per primer were recorded. The polymorphism information content ranged from 0.102 (OPAI-01) to 0.517 (OPE-04). A dendrogram divided the genotypes into two major and further into sub-groups according to their collection center geographical regions. This was the first report on molecular diversity analysis of a large collection of little millet germplasm. Diverse genotypes may be used in breeding programmes for P. sumatrense improvement." @default.
- W2895925917 created "2018-10-26" @default.
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- W2895925917 date "2018-08-01" @default.
- W2895925917 modified "2023-10-09" @default.
- W2895925917 title "Genetic characterization of Indian little millet (Panicum sumatrense) genotypes using random amplified polymorphic DNA markers" @default.
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- W2895925917 doi "https://doi.org/10.1016/j.anres.2018.10.008" @default.
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