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- W2895933397 abstract "Connexin 43(Cx43) widely exists in all components of the neurovascular unit and is a constituent of gap junctions and hemichannels. In physiological states, gap junctions are open for regular intercellular communication, and the hemichannels present low open probability in astrocytes. After cerebral ischemia, a large number of hemichannels are unusually opened, leading to cell swelling and even death. Most known hemichannel blockers also inhibit gap junctions and sequentially obstruct normal electrical cell-cell communication. In this study, we tested the hypothesis that Gap19, a selective Cx43-hemichannel inhibitor, exhibited neuroprotective effects on cerebral ischemia/reperfusion (I/R). An obvious improvement in neurological scores and infarct volume reduction were observed in Gap19-treated mice after brain ischemia induced by middle cerebral artery occlusion (MCAO). Gap19 treatment attenuated white matter damage. Moreover, Gap19 treatment suppressed the expression of Cx43 and TLR4 pathway-relevant proteins and prevented the overexpression of TNF-α and IL-1β. To further explore downstream signaling, we established an in vitro model--oxygen glucose deprivation (OGD) to simulate ischemic conditions. Immunofluorescence staining showed that Cx43 co-existed with TLR4 in astrocytes. The hemichannel activity was increased after OGD and Gap19 could inhibit this effect on astrocytes. Gap19 substantially improved relative cell vitality and decreased the expression of Cx43, TLR4 and inflammatory cytokines in vitro. In addition, in the LPS stimulation OGD model, Gap19 also exhibited a protective effect via inhibiting TLR4 pathway activation. In summary, our results showed that Gap19 exerted a neuroprotective effect after stroke via inhibition of the TLR4-mediated signaling pathway." @default.
- W2895933397 created "2018-10-26" @default.
- W2895933397 creator A5000645986 @default.
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- W2895933397 date "2018-10-17" @default.
- W2895933397 modified "2023-10-16" @default.
- W2895933397 title "Inhibition of Connexin 43 Hemichannels Alleviates Cerebral Ischemia/Reperfusion Injury via the TLR4 Signaling Pathway" @default.
- W2895933397 cites W1568910178 @default.
- W2895933397 cites W1570729614 @default.
- W2895933397 cites W1790030986 @default.
- W2895933397 cites W1974444929 @default.
- W2895933397 cites W1976010325 @default.
- W2895933397 cites W1981984722 @default.
- W2895933397 cites W1984920952 @default.
- W2895933397 cites W1984989438 @default.
- W2895933397 cites W1995041648 @default.
- W2895933397 cites W1998432972 @default.
- W2895933397 cites W2002965338 @default.
- W2895933397 cites W2004149652 @default.
- W2895933397 cites W2005695657 @default.
- W2895933397 cites W2006612479 @default.
- W2895933397 cites W2014431033 @default.
- W2895933397 cites W2016575029 @default.
- W2895933397 cites W2022219828 @default.
- W2895933397 cites W2023720530 @default.
- W2895933397 cites W2025461456 @default.
- W2895933397 cites W2026332221 @default.
- W2895933397 cites W2026489774 @default.
- W2895933397 cites W2033189137 @default.
- W2895933397 cites W2034264974 @default.
- W2895933397 cites W2034741420 @default.
- W2895933397 cites W2036455407 @default.
- W2895933397 cites W203926185 @default.
- W2895933397 cites W2040062409 @default.
- W2895933397 cites W2041380818 @default.
- W2895933397 cites W2041604665 @default.
- W2895933397 cites W2047460643 @default.
- W2895933397 cites W2051367002 @default.
- W2895933397 cites W2054945824 @default.
- W2895933397 cites W2056086402 @default.
- W2895933397 cites W2063541896 @default.
- W2895933397 cites W2066158538 @default.
- W2895933397 cites W2070627442 @default.
- W2895933397 cites W2070896918 @default.
- W2895933397 cites W2076996597 @default.
- W2895933397 cites W2080960094 @default.
- W2895933397 cites W2081022657 @default.
- W2895933397 cites W2085123274 @default.
- W2895933397 cites W2090064193 @default.
- W2895933397 cites W2091080691 @default.
- W2895933397 cites W2092530725 @default.
- W2895933397 cites W2093318093 @default.
- W2895933397 cites W2093958758 @default.
- W2895933397 cites W2096545764 @default.
- W2895933397 cites W2104591424 @default.
- W2895933397 cites W2108790161 @default.
- W2895933397 cites W2109491705 @default.
- W2895933397 cites W2115387979 @default.
- W2895933397 cites W2118549246 @default.
- W2895933397 cites W2120742009 @default.
- W2895933397 cites W2121355349 @default.
- W2895933397 cites W2124439764 @default.
- W2895933397 cites W2134219545 @default.
- W2895933397 cites W2139112966 @default.
- W2895933397 cites W2139966536 @default.
- W2895933397 cites W2141752309 @default.
- W2895933397 cites W2146044361 @default.
- W2895933397 cites W2149741922 @default.
- W2895933397 cites W2155277431 @default.
- W2895933397 cites W2168631647 @default.
- W2895933397 cites W2171165037 @default.
- W2895933397 cites W2177563320 @default.
- W2895933397 cites W2181566246 @default.
- W2895933397 cites W2298852151 @default.
- W2895933397 cites W2371948111 @default.
- W2895933397 cites W2402738280 @default.
- W2895933397 cites W2403366498 @default.
- W2895933397 cites W2517744753 @default.
- W2895933397 cites W2558979571 @default.
- W2895933397 cites W2562525888 @default.
- W2895933397 cites W2584646669 @default.
- W2895933397 cites W2613702240 @default.
- W2895933397 cites W2782184507 @default.
- W2895933397 cites W2792583460 @default.
- W2895933397 cites W2801240606 @default.
- W2895933397 cites W4255683973 @default.
- W2895933397 cites W4293084779 @default.
- W2895933397 cites W642235081 @default.
- W2895933397 doi "https://doi.org/10.3389/fncel.2018.00372" @default.
- W2895933397 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6199357" @default.
- W2895933397 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30386214" @default.
- W2895933397 hasPublicationYear "2018" @default.
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