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• W2895940493 abstract "Abstract The spread of carbapenemase-producing Enterobacteriaceae (CPE) is an increasing global public health concern. The development of simple and reliable methods for CPE detection is required in the clinical setting. This study aimed to establish a dual-wavelength measurement method using an ultraviolet–visible spectrophotometer to rapidly quantify imipenem hydrolysis in bacterial cell suspensions. The hydrolytic activities of 148 strains including various CPE strains ( Escherichia coli , Klebsiella pneumoniae , Enterobacter cloacae , and Enterobacter aerogenes containing the bla IMP , bla KPC , bla NDM , bla OXA , and bla VIM genes) were measured and analysed. A cut-off value was obtained for differentiation between CPE and non-CPE strains, and the method had high sensitivity (100%) and specificity (100%) within 60 min. Our system has potential clinical applications in detecting CPE." @default.
• W2895940493 created "2018-10-26" @default.
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• W2895940493 date "2018-10-24" @default.
• W2895940493 modified "2023-10-14" @default.
• W2895940493 title "Establishment of a dual-wavelength spectrophotometric method for analysing and detecting carbapenemase-producing Enterobacteriaceae" @default.
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• W2895940493 doi "https://doi.org/10.1038/s41598-018-33883-0" @default.
• W2895940493 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6200816" @default.
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