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- W2895979708 abstract "Thesearch for a sustainable and environmental friendly energy has resulted to thedevelopment of an alternative renewable energy such as biogas. The objective ofthis work was to identify the presence of methanogenic bacteria in a short-termoperated biogas reactor fed with kitchen waste by PCR-amplification of themethyl coenzyme M reductase (mcrA). Household organic waste wasobtained from Bells University of Technology canteen while pig manure thatserved as the inoculum was obtained from a pig farm. Three replicate sampleswere taken every week and analysed for changes in the physico-chemicalparameters to determine their effects on the rate of biogas production. Resultsobtained showed that the % moisture and carbon contents, before and afterfermentation, were reduced from 88% to 80% and16.7% to 12.5%, while % nitrogen content increased from 1.20% to 1.70%. Themean weekly biogas production ranged from 27.4 ml to 147 ml at 4 weeks and 6weeks, respectively. Methyl coenzyme M reductase(mcrA) gene, which codes for the enzyme thatcatalyse the terminal step in methane production during the anaerobicfermentation of biomass, was amplified both inthe methanogenic bacteria and transformed E.coli with the fragment size of 500 bp." @default.
- W2895979708 created "2018-10-26" @default.
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- W2895979708 date "2018-06-01" @default.
- W2895979708 modified "2023-10-18" @default.
- W2895979708 title "Molecular Detection of Biogenic Bacteria During Biogas Production Using Domestic Feed Stock" @default.
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- W2895979708 doi "https://doi.org/10.21608/eajbsg.2018.16312" @default.
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