Matches in SemOpenAlex for { <https://semopenalex.org/work/W2895988846> ?p ?o ?g. }
- W2895988846 endingPage "232" @default.
- W2895988846 startingPage "226" @default.
- W2895988846 abstract "Nucleic acids labeled with a fluorophore/quencher pair are widely used as probes in biomedical research and molecular diagnostics. Here we synthesized novel DNA molecular beacons double labeled with the identical dyes (R6G, ROX and Cy5) at 5′- and 3′-end and studied their photo physical properties. We demonstrated that fluorescence quenching by formation of the homo dimer exciton in such molecular beacons allows using them in homogeneous assays. Further, we developed and evaluated homo Yin-Yang DNA probes labeled with identical dyes and used them for detection of low copy HIV RNA by RT-qPCR. They demonstrated improved sensitivity (LLQ: 10 vs 30 copies mL-1) in comparison to commercially available Abbott RealTime HIV-1 kit based on VIC-BHQ dyes both for model mixtures (naive human plasma with added deactivated HIV-1 virus) and for preliminarily confirmed 36 clinical samples (4 vs 1 positive ones for low-copy samples)." @default.
- W2895988846 created "2018-10-26" @default.
- W2895988846 creator A5000564880 @default.
- W2895988846 creator A5014865801 @default.
- W2895988846 creator A5015218296 @default.
- W2895988846 creator A5018640748 @default.
- W2895988846 creator A5021493021 @default.
- W2895988846 creator A5053880971 @default.
- W2895988846 creator A5056763236 @default.
- W2895988846 creator A5060272830 @default.
- W2895988846 creator A5066393684 @default.
- W2895988846 creator A5068073013 @default.
- W2895988846 date "2019-03-01" @default.
- W2895988846 modified "2023-10-03" @default.
- W2895988846 title "Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA" @default.
- W2895988846 cites W1215357893 @default.
- W2895988846 cites W1480060486 @default.
- W2895988846 cites W1557966683 @default.
- W2895988846 cites W1742676908 @default.
- W2895988846 cites W1970070502 @default.
- W2895988846 cites W1972895153 @default.
- W2895988846 cites W1986027247 @default.
- W2895988846 cites W1988966853 @default.
- W2895988846 cites W1993214867 @default.
- W2895988846 cites W1993618439 @default.
- W2895988846 cites W2007025001 @default.
- W2895988846 cites W2012530253 @default.
- W2895988846 cites W2014030740 @default.
- W2895988846 cites W2023395165 @default.
- W2895988846 cites W2032305454 @default.
- W2895988846 cites W2065263621 @default.
- W2895988846 cites W2068535838 @default.
- W2895988846 cites W2069012786 @default.
- W2895988846 cites W2081388911 @default.
- W2895988846 cites W2081567960 @default.
- W2895988846 cites W2092214053 @default.
- W2895988846 cites W2094253593 @default.
- W2895988846 cites W2105944077 @default.
- W2895988846 cites W2106383584 @default.
- W2895988846 cites W2113193087 @default.
- W2895988846 cites W2130243293 @default.
- W2895988846 cites W2135817740 @default.
- W2895988846 cites W2136387231 @default.
- W2895988846 cites W2139077271 @default.
- W2895988846 cites W2142197723 @default.
- W2895988846 cites W2147550343 @default.
- W2895988846 cites W2147855159 @default.
- W2895988846 cites W2149701672 @default.
- W2895988846 cites W2158040617 @default.
- W2895988846 cites W2163000974 @default.
- W2895988846 cites W2165714458 @default.
- W2895988846 cites W2170087558 @default.
- W2895988846 cites W2185990175 @default.
- W2895988846 cites W2209362474 @default.
- W2895988846 cites W2316251002 @default.
- W2895988846 cites W2325730293 @default.
- W2895988846 cites W2339150642 @default.
- W2895988846 cites W2402980652 @default.
- W2895988846 cites W2605528968 @default.
- W2895988846 cites W2612664435 @default.
- W2895988846 cites W2754826667 @default.
- W2895988846 cites W2799566423 @default.
- W2895988846 cites W2950397606 @default.
- W2895988846 cites W2950621151 @default.
- W2895988846 doi "https://doi.org/10.1016/j.talanta.2018.10.043" @default.
- W2895988846 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30609524" @default.
- W2895988846 hasPublicationYear "2019" @default.
- W2895988846 type Work @default.
- W2895988846 sameAs 2895988846 @default.
- W2895988846 citedByCount "9" @default.
- W2895988846 countsByYear W28959888462019 @default.
- W2895988846 countsByYear W28959888462020 @default.
- W2895988846 countsByYear W28959888462021 @default.
- W2895988846 countsByYear W28959888462022 @default.
- W2895988846 countsByYear W28959888462023 @default.
- W2895988846 crossrefType "journal-article" @default.
- W2895988846 hasAuthorship W2895988846A5000564880 @default.
- W2895988846 hasAuthorship W2895988846A5014865801 @default.
- W2895988846 hasAuthorship W2895988846A5015218296 @default.
- W2895988846 hasAuthorship W2895988846A5018640748 @default.
- W2895988846 hasAuthorship W2895988846A5021493021 @default.
- W2895988846 hasAuthorship W2895988846A5053880971 @default.
- W2895988846 hasAuthorship W2895988846A5056763236 @default.
- W2895988846 hasAuthorship W2895988846A5060272830 @default.
- W2895988846 hasAuthorship W2895988846A5066393684 @default.
- W2895988846 hasAuthorship W2895988846A5068073013 @default.
- W2895988846 hasConcept C104317684 @default.
- W2895988846 hasConcept C119203544 @default.
- W2895988846 hasConcept C121332964 @default.
- W2895988846 hasConcept C121745418 @default.
- W2895988846 hasConcept C12554922 @default.
- W2895988846 hasConcept C129312508 @default.
- W2895988846 hasConcept C153911025 @default.
- W2895988846 hasConcept C159047783 @default.
- W2895988846 hasConcept C185592680 @default.
- W2895988846 hasConcept C24107716 @default.
- W2895988846 hasConcept C2778617687 @default.
- W2895988846 hasConcept C3013748606 @default.