FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896007618> ?p ?o ?g. }

• W2896007618 abstract "Adipose derived stem cells can be stimulated to produce a growth factor rich secretome which enhances axon regeneration. In this study we investigated the importance of exosomes, extracellular vesicles released by many different cell types, including stem cells and endogenous nervous system Schwann cells (SCs), on neurite outgrowth.Adipose derived stem cells were differentiated towards a Schwann cell-like phenotype (dADSCs) by in vitro stimulation with a mix of factors (basic fibroblast growth factor, platelet derived growth factor-AA, neuregulin-1 and forskolin). Using a precipitation and low-speed centrifugation protocol the extracellular vesicles were isolated from the medium of the stem cells cultures and also from primary SCs. The conditioned media or concentrated vesicles were applied to neurons in vitro and computerised image analysis was used to assess neurite outgrowth. Total RNA was purified from the extracellular vesicles and investigated using qRT-PCR.Application of exosomes derived from SCs significantly enhanced in vitro neurite outgrowth and this was replicated by the exosomes from dADSCs. qRT-PCR demonstrated that the exosomes contained mRNAs and miRNAs known to play a role in nerve regeneration and these molecules were up-regulated by the Schwann cell differentiation protocol. Transfer of fluorescently tagged exosomal RNA to neurons was detected and destruction of the RNA by UV-irradiation significantly reduced the dADSCs exosome effects on neurite outgrowth. In contrast, this process had no significant effect on the SCs-derived exosomes.In summary, this work suggests that stem cell-derived exosomes might be a useful adjunct to other novel therapeutic interventions in nerve repair." @default.
• W2896007618 created "2018-10-26" @default.
• W2896007618 creator A5053274960 @default.
• W2896007618 creator A5074803524 @default.
• W2896007618 creator A5081317055 @default.
• W2896007618 date "2018-10-11" @default.
• W2896007618 modified "2023-10-17" @default.
• W2896007618 title "Schwann cell-like differentiated adipose stem cells promote neurite outgrowth via secreted exosomes and RNA transfer" @default.
• W2896007618 cites W1600289867 @default.
• W2896007618 cites W1894141023 @default.
• W2896007618 cites W1906233383 @default.
• W2896007618 cites W1938236935 @default.
• W2896007618 cites W1965235470 @default.
• W2896007618 cites W1965932992 @default.
• W2896007618 cites W1968300632 @default.
• W2896007618 cites W1971107027 @default.
• W2896007618 cites W1971790889 @default.
• W2896007618 cites W1974163213 @default.
• W2896007618 cites W1985498551 @default.
• W2896007618 cites W1989165794 @default.
• W2896007618 cites W1990388063 @default.
• W2896007618 cites W1991570175 @default.
• W2896007618 cites W2007499410 @default.
• W2896007618 cites W2012528990 @default.
• W2896007618 cites W2013645766 @default.
• W2896007618 cites W2024451001 @default.
• W2896007618 cites W2026570544 @default.
• W2896007618 cites W2030100470 @default.
• W2896007618 cites W2032281007 @default.
• W2896007618 cites W2039042758 @default.
• W2896007618 cites W2040250419 @default.
• W2896007618 cites W2041302930 @default.
• W2896007618 cites W2051931680 @default.
• W2896007618 cites W2051995151 @default.
• W2896007618 cites W2053436333 @default.
• W2896007618 cites W2054939463 @default.
• W2896007618 cites W2055313665 @default.
• W2896007618 cites W2058092020 @default.
• W2896007618 cites W2060115989 @default.
• W2896007618 cites W2064091007 @default.
• W2896007618 cites W2067432992 @default.
• W2896007618 cites W2079782815 @default.
• W2896007618 cites W2083156208 @default.
• W2896007618 cites W2085103168 @default.
• W2896007618 cites W2085554170 @default.
• W2896007618 cites W2114721953 @default.
• W2896007618 cites W2121354606 @default.
• W2896007618 cites W2121882633 @default.
• W2896007618 cites W2123628900 @default.
• W2896007618 cites W2126974532 @default.
• W2896007618 cites W2127078121 @default.
• W2896007618 cites W2129431919 @default.
• W2896007618 cites W2132277181 @default.
• W2896007618 cites W2132621835 @default.
• W2896007618 cites W2134806200 @default.
• W2896007618 cites W2142202546 @default.
• W2896007618 cites W2142410730 @default.
• W2896007618 cites W2156229286 @default.
• W2896007618 cites W2159739718 @default.
• W2896007618 cites W2163711839 @default.
• W2896007618 cites W2169956387 @default.
• W2896007618 cites W2170852633 @default.
• W2896007618 cites W2171376759 @default.
• W2896007618 cites W2468489993 @default.
• W2896007618 cites W2552941476 @default.
• W2896007618 cites W2567035970 @default.
• W2896007618 cites W2614462948 @default.
• W2896007618 cites W2625285338 @default.
• W2896007618 cites W2806231407 @default.
• W2896007618 cites W2809583534 @default.
• W2896007618 doi "https://doi.org/10.1186/s13287-018-1017-8" @default.
• W2896007618 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6182785" @default.
• W2896007618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30309388" @default.
• W2896007618 hasPublicationYear "2018" @default.
• W2896007618 type Work @default.
• W2896007618 sameAs 2896007618 @default.
• W2896007618 citedByCount "88" @default.
• W2896007618 countsByYear W28960076182018 @default.
• W2896007618 countsByYear W28960076182019 @default.
• W2896007618 countsByYear W28960076182020 @default.
• W2896007618 countsByYear W28960076182021 @default.
• W2896007618 countsByYear W28960076182022 @default.
• W2896007618 countsByYear W28960076182023 @default.
• W2896007618 crossrefType "journal-article" @default.
• W2896007618 hasAuthorship W2896007618A5053274960 @default.
• W2896007618 hasAuthorship W2896007618A5074803524 @default.
• W2896007618 hasAuthorship W2896007618A5081317055 @default.
• W2896007618 hasBestOaLocation W28960076181 @default.
• W2896007618 hasConcept C104317684 @default.
• W2896007618 hasConcept C113246987 @default.
• W2896007618 hasConcept C145059251 @default.
• W2896007618 hasConcept C202751555 @default.
• W2896007618 hasConcept C20518536 @default.
• W2896007618 hasConcept C2781261824 @default.
• W2896007618 hasConcept C28328180 @default.
• W2896007618 hasConcept C55493867 @default.
• W2896007618 hasConcept C86803240 @default.
• W2896007618 hasConcept C95444343 @default.
• W2896007618 hasConceptScore W2896007618C104317684 @default.
• W2896007618 hasConceptScore W2896007618C113246987 @default.

• next