Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896066345> ?p ?o ?g. }
- W2896066345 endingPage "479" @default.
- W2896066345 startingPage "469" @default.
- W2896066345 abstract "The type III interferon (IFN-λ) family includes 4 IFN-λ subtypes in man. In the mouse, only the genes coding for IFN-λ2 and -λ3 are present. Unlike mouse and human type I IFNs (IFN-α/β), which exhibit strong species specificity, type III IFNs were reported to act in a cross-specific manner. We reexamined the cross-specificity and observed that mouse and human IFN-λ exhibit some species specificity, although much less than type I IFNs. Mouse IFN-λ3 displayed clear species specificity, being 25-fold less active in human cells than the closely related mouse IFN-λ2. This specificity likely depends on amino acids in α helices A and F that diverged from other IFN-λ sequences. Human IFN-λ4, in contrast, retained high activity in mouse cells. We next developed a firefly luciferase-based reporter cell line, named Fawa-λ-luc, to detect IFN-λ in biological fluids with high specificity and sensitivity. Fawa-λ-luc cells, derived from mouse epithelial cells that are responsive to IFN-λ, were made nonresponsive to type I IFNs by inactivation of the Ifnar2 gene and strongly responsive to IFN-λ by overexpression of the mouse IFNLR1. This bioassay was as sensitive as a commercially available enzyme-linked immunosorbent assay in detecting mouse IFN-λ in cell culture supernatant, as well as in serum and bronchoalveolar lavage samples of virus-infected mice. The assay also enabled the sensitive detection of human IFN-λ activity, including that of the divergent IFN-λ4 with a bias, however, due to variable activity of IFN-λ subtypes." @default.
- W2896066345 created "2018-10-26" @default.
- W2896066345 creator A5005051503 @default.
- W2896066345 creator A5023297727 @default.
- W2896066345 creator A5036604415 @default.
- W2896066345 creator A5041933832 @default.
- W2896066345 creator A5055506675 @default.
- W2896066345 creator A5067904572 @default.
- W2896066345 creator A5070224625 @default.
- W2896066345 creator A5089010495 @default.
- W2896066345 creator A5089822643 @default.
- W2896066345 date "2018-11-01" @default.
- W2896066345 modified "2023-10-02" @default.
- W2896066345 title "Species Specificity of Type III Interferon Activity and Development of a Sensitive Luciferase-Based Bioassay for Quantitation of Mouse Interferon-λ" @default.
- W2896066345 cites W1204429840 @default.
- W2896066345 cites W1566969252 @default.
- W2896066345 cites W1607246256 @default.
- W2896066345 cites W1611210979 @default.
- W2896066345 cites W1834477603 @default.
- W2896066345 cites W1977709885 @default.
- W2896066345 cites W1978717719 @default.
- W2896066345 cites W1987009823 @default.
- W2896066345 cites W2013202475 @default.
- W2896066345 cites W2016505381 @default.
- W2896066345 cites W2017741366 @default.
- W2896066345 cites W2026951628 @default.
- W2896066345 cites W2037863726 @default.
- W2896066345 cites W2060926084 @default.
- W2896066345 cites W2063325766 @default.
- W2896066345 cites W2079771032 @default.
- W2896066345 cites W2083733004 @default.
- W2896066345 cites W2085237060 @default.
- W2896066345 cites W2085242582 @default.
- W2896066345 cites W2086409213 @default.
- W2896066345 cites W2093336578 @default.
- W2896066345 cites W2107830237 @default.
- W2896066345 cites W2114067488 @default.
- W2896066345 cites W2114860838 @default.
- W2896066345 cites W2115528382 @default.
- W2896066345 cites W2120104259 @default.
- W2896066345 cites W2127951127 @default.
- W2896066345 cites W2153391962 @default.
- W2896066345 cites W2157135427 @default.
- W2896066345 cites W2164157473 @default.
- W2896066345 cites W2167380395 @default.
- W2896066345 cites W2209458064 @default.
- W2896066345 cites W2606342359 @default.
- W2896066345 cites W2745352569 @default.
- W2896066345 cites W2763533536 @default.
- W2896066345 cites W2767687471 @default.
- W2896066345 doi "https://doi.org/10.1089/jir.2018.0066" @default.
- W2896066345 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6249671" @default.
- W2896066345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30335553" @default.
- W2896066345 hasPublicationYear "2018" @default.
- W2896066345 type Work @default.
- W2896066345 sameAs 2896066345 @default.
- W2896066345 citedByCount "9" @default.
- W2896066345 countsByYear W28960663452019 @default.
- W2896066345 countsByYear W28960663452020 @default.
- W2896066345 countsByYear W28960663452021 @default.
- W2896066345 countsByYear W28960663452022 @default.
- W2896066345 countsByYear W28960663452023 @default.
- W2896066345 crossrefType "journal-article" @default.
- W2896066345 hasAuthorship W2896066345A5005051503 @default.
- W2896066345 hasAuthorship W2896066345A5023297727 @default.
- W2896066345 hasAuthorship W2896066345A5036604415 @default.
- W2896066345 hasAuthorship W2896066345A5041933832 @default.
- W2896066345 hasAuthorship W2896066345A5055506675 @default.
- W2896066345 hasAuthorship W2896066345A5067904572 @default.
- W2896066345 hasAuthorship W2896066345A5070224625 @default.
- W2896066345 hasAuthorship W2896066345A5089010495 @default.
- W2896066345 hasAuthorship W2896066345A5089822643 @default.
- W2896066345 hasBestOaLocation W28960663451 @default.
- W2896066345 hasConcept C104317684 @default.
- W2896066345 hasConcept C104488531 @default.
- W2896066345 hasConcept C111335760 @default.
- W2896066345 hasConcept C143065580 @default.
- W2896066345 hasConcept C150194340 @default.
- W2896066345 hasConcept C153911025 @default.
- W2896066345 hasConcept C159047783 @default.
- W2896066345 hasConcept C161733203 @default.
- W2896066345 hasConcept C207583985 @default.
- W2896066345 hasConcept C2776178377 @default.
- W2896066345 hasConcept C2778278966 @default.
- W2896066345 hasConcept C40767141 @default.
- W2896066345 hasConcept C54009773 @default.
- W2896066345 hasConcept C54355233 @default.
- W2896066345 hasConcept C55493867 @default.
- W2896066345 hasConcept C81885089 @default.
- W2896066345 hasConcept C86803240 @default.
- W2896066345 hasConceptScore W2896066345C104317684 @default.
- W2896066345 hasConceptScore W2896066345C104488531 @default.
- W2896066345 hasConceptScore W2896066345C111335760 @default.
- W2896066345 hasConceptScore W2896066345C143065580 @default.
- W2896066345 hasConceptScore W2896066345C150194340 @default.
- W2896066345 hasConceptScore W2896066345C153911025 @default.
- W2896066345 hasConceptScore W2896066345C159047783 @default.
- W2896066345 hasConceptScore W2896066345C161733203 @default.