Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896070158> ?p ?o ?g. }
- W2896070158 endingPage "671" @default.
- W2896070158 startingPage "662" @default.
- W2896070158 abstract "A natural peptide motif in the first helix of osteocalcin (OCN) is used to promote nucleation and crystallization of hydroxyapatite (HA) in hard tissue. The capability of osteocalcin mimetic peptides to induce osteogenic activity of osteoblast cells leading to in-vitro mineralization is demonstrated. An osteocalcin-derived peptide consisting of thirteen amino acids is synthesized in both acidic (OSC) and amidic (OSN) forms and added into the human osteoblast-like cells (MG63) culture. The viability, proliferation, alkaline phosphatase activity, HA deposition and osteogenic gene expression by osteoblast cells are evaluated. It is revealed that the addition of 100 μg/ml of peptides enhances the proliferation rate and total protein content of osteoblast cells. Alkaline phosphatase activity is significantly higher in the presence of peptides which in turn stimulated RNA expression of collagen type I and osteopontin in a phosphate-dependent manner. Alizarin red staining and calcium content measurement show that mineral deposition is considerably increased. Ultrastructural characterization of MG63 cultures confirms the crystalline nature and chemical composition of HA mineral formation in the presence of peptides. It is confirmed that the osteocalcin-derived peptide, particularly in amidic form (OSN), is able to act as a bioactive inducer of mineralization process and hence accelerating bone tissue regeneration." @default.
- W2896070158 created "2018-10-26" @default.
- W2896070158 creator A5007285706 @default.
- W2896070158 creator A5027238051 @default.
- W2896070158 creator A5034720645 @default.
- W2896070158 creator A5044188339 @default.
- W2896070158 creator A5066959814 @default.
- W2896070158 creator A5067658355 @default.
- W2896070158 creator A5087931532 @default.
- W2896070158 date "2019-01-01" @default.
- W2896070158 modified "2023-10-12" @default.
- W2896070158 title "Contribution of osteocalcin-mimetic peptide enhances osteogenic activity and extracellular matrix mineralization of human osteoblast-like cells" @default.
- W2896070158 cites W1205478764 @default.
- W2896070158 cites W1544003988 @default.
- W2896070158 cites W1754620318 @default.
- W2896070158 cites W1912515756 @default.
- W2896070158 cites W1965876889 @default.
- W2896070158 cites W1967384026 @default.
- W2896070158 cites W1968700701 @default.
- W2896070158 cites W1977820970 @default.
- W2896070158 cites W1984706219 @default.
- W2896070158 cites W1986469118 @default.
- W2896070158 cites W1990584980 @default.
- W2896070158 cites W1995911293 @default.
- W2896070158 cites W1998351418 @default.
- W2896070158 cites W1999824144 @default.
- W2896070158 cites W2003776109 @default.
- W2896070158 cites W2012333093 @default.
- W2896070158 cites W2015203259 @default.
- W2896070158 cites W2019487320 @default.
- W2896070158 cites W2027852937 @default.
- W2896070158 cites W2028967615 @default.
- W2896070158 cites W2032385224 @default.
- W2896070158 cites W2037850865 @default.
- W2896070158 cites W2040057440 @default.
- W2896070158 cites W2042960552 @default.
- W2896070158 cites W2053288021 @default.
- W2896070158 cites W2058370892 @default.
- W2896070158 cites W2059564448 @default.
- W2896070158 cites W2059717012 @default.
- W2896070158 cites W2067719770 @default.
- W2896070158 cites W2068674448 @default.
- W2896070158 cites W2081023457 @default.
- W2896070158 cites W2092230369 @default.
- W2896070158 cites W2102179078 @default.
- W2896070158 cites W2107727273 @default.
- W2896070158 cites W2109441210 @default.
- W2896070158 cites W2109623522 @default.
- W2896070158 cites W2114528551 @default.
- W2896070158 cites W2125538936 @default.
- W2896070158 cites W2139392047 @default.
- W2896070158 cites W2141861066 @default.
- W2896070158 cites W2152272441 @default.
- W2896070158 cites W2165715446 @default.
- W2896070158 cites W2168040808 @default.
- W2896070158 cites W2217100198 @default.
- W2896070158 cites W2469856186 @default.
- W2896070158 doi "https://doi.org/10.1016/j.colsurfb.2018.10.035" @default.
- W2896070158 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30368214" @default.
- W2896070158 hasPublicationYear "2019" @default.
- W2896070158 type Work @default.
- W2896070158 sameAs 2896070158 @default.
- W2896070158 citedByCount "29" @default.
- W2896070158 countsByYear W28960701582019 @default.
- W2896070158 countsByYear W28960701582020 @default.
- W2896070158 countsByYear W28960701582021 @default.
- W2896070158 countsByYear W28960701582022 @default.
- W2896070158 countsByYear W28960701582023 @default.
- W2896070158 crossrefType "journal-article" @default.
- W2896070158 hasAuthorship W2896070158A5007285706 @default.
- W2896070158 hasAuthorship W2896070158A5027238051 @default.
- W2896070158 hasAuthorship W2896070158A5034720645 @default.
- W2896070158 hasAuthorship W2896070158A5044188339 @default.
- W2896070158 hasAuthorship W2896070158A5066959814 @default.
- W2896070158 hasAuthorship W2896070158A5067658355 @default.
- W2896070158 hasAuthorship W2896070158A5087931532 @default.
- W2896070158 hasConcept C111696902 @default.
- W2896070158 hasConcept C131075544 @default.
- W2896070158 hasConcept C134018914 @default.
- W2896070158 hasConcept C153911025 @default.
- W2896070158 hasConcept C160160445 @default.
- W2896070158 hasConcept C178790620 @default.
- W2896070158 hasConcept C181199279 @default.
- W2896070158 hasConcept C185592680 @default.
- W2896070158 hasConcept C189165786 @default.
- W2896070158 hasConcept C202751555 @default.
- W2896070158 hasConcept C2778260815 @default.
- W2896070158 hasConcept C2779281246 @default.
- W2896070158 hasConcept C2780804394 @default.
- W2896070158 hasConcept C537208039 @default.
- W2896070158 hasConcept C55493867 @default.
- W2896070158 hasConcept C86803240 @default.
- W2896070158 hasConcept C95444343 @default.
- W2896070158 hasConceptScore W2896070158C111696902 @default.
- W2896070158 hasConceptScore W2896070158C131075544 @default.
- W2896070158 hasConceptScore W2896070158C134018914 @default.
- W2896070158 hasConceptScore W2896070158C153911025 @default.
- W2896070158 hasConceptScore W2896070158C160160445 @default.