Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896073378> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2896073378 abstract "Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial world. We have previously discovered that higher barriers to the development of drug resistance can be achieved through dominant suppression of drug-resistant viruses by their drug-susceptible parents. We have explored the existence of dominant drug targets in poliovirus, dengue virus and hepatitis C virus (HCV). The low replication capacity of HCV required the development of novel strategies for identifying cells co-infected with drug-susceptible and drug-resistant strains. To monitor co-infected cell populations, we generated codon-altered versions of the JFH1 strain of HCV. Then, we could differentiate the codon-altered and wild-type strains using a novel type of RNA fluorescent in situ hybridization (FISH) coupled with flow cytometry or confocal microscopy. Both of these techniques can be used in conjunction with standard antibody-protein detection methods. Here, we describe a detailed protocol for both RNA FISH flow cytometry and confocal microscopy." @default.
- W2896073378 created "2018-10-26" @default.
- W2896073378 creator A5012690304 @default.
- W2896073378 creator A5073092630 @default.
- W2896073378 date "2018-01-01" @default.
- W2896073378 modified "2023-09-27" @default.
- W2896073378 title "Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry" @default.
- W2896073378 cites W2002167317 @default.
- W2896073378 cites W2020021414 @default.
- W2896073378 cites W2169878750 @default.
- W2896073378 cites W2212166949 @default.
- W2896073378 cites W2283835306 @default.
- W2896073378 cites W2795114385 @default.
- W2896073378 doi "https://doi.org/10.21769/bioprotoc.3058" @default.
- W2896073378 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6269108" @default.
- W2896073378 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30505886" @default.
- W2896073378 hasPublicationYear "2018" @default.
- W2896073378 type Work @default.
- W2896073378 sameAs 2896073378 @default.
- W2896073378 citedByCount "5" @default.
- W2896073378 countsByYear W28960733782018 @default.
- W2896073378 countsByYear W28960733782020 @default.
- W2896073378 countsByYear W28960733782021 @default.
- W2896073378 countsByYear W28960733782022 @default.
- W2896073378 crossrefType "journal-article" @default.
- W2896073378 hasAuthorship W2896073378A5012690304 @default.
- W2896073378 hasAuthorship W2896073378A5073092630 @default.
- W2896073378 hasBestOaLocation W28960733781 @default.
- W2896073378 hasConcept C104317684 @default.
- W2896073378 hasConcept C140704245 @default.
- W2896073378 hasConcept C153911025 @default.
- W2896073378 hasConcept C159047783 @default.
- W2896073378 hasConcept C2522874641 @default.
- W2896073378 hasConcept C2777542201 @default.
- W2896073378 hasConcept C2779178360 @default.
- W2896073378 hasConcept C2779756493 @default.
- W2896073378 hasConcept C30481170 @default.
- W2896073378 hasConcept C54355233 @default.
- W2896073378 hasConcept C553184892 @default.
- W2896073378 hasConcept C67705224 @default.
- W2896073378 hasConcept C86803240 @default.
- W2896073378 hasConcept C95444343 @default.
- W2896073378 hasConceptScore W2896073378C104317684 @default.
- W2896073378 hasConceptScore W2896073378C140704245 @default.
- W2896073378 hasConceptScore W2896073378C153911025 @default.
- W2896073378 hasConceptScore W2896073378C159047783 @default.
- W2896073378 hasConceptScore W2896073378C2522874641 @default.
- W2896073378 hasConceptScore W2896073378C2777542201 @default.
- W2896073378 hasConceptScore W2896073378C2779178360 @default.
- W2896073378 hasConceptScore W2896073378C2779756493 @default.
- W2896073378 hasConceptScore W2896073378C30481170 @default.
- W2896073378 hasConceptScore W2896073378C54355233 @default.
- W2896073378 hasConceptScore W2896073378C553184892 @default.
- W2896073378 hasConceptScore W2896073378C67705224 @default.
- W2896073378 hasConceptScore W2896073378C86803240 @default.
- W2896073378 hasConceptScore W2896073378C95444343 @default.
- W2896073378 hasIssue "20" @default.
- W2896073378 hasLocation W28960733781 @default.
- W2896073378 hasLocation W28960733782 @default.
- W2896073378 hasLocation W28960733783 @default.
- W2896073378 hasLocation W28960733784 @default.
- W2896073378 hasOpenAccess W2896073378 @default.
- W2896073378 hasPrimaryLocation W28960733781 @default.
- W2896073378 hasRelatedWork W1487857142 @default.
- W2896073378 hasRelatedWork W2096288449 @default.
- W2896073378 hasRelatedWork W2112481771 @default.
- W2896073378 hasRelatedWork W2142935324 @default.
- W2896073378 hasRelatedWork W2744966359 @default.
- W2896073378 hasRelatedWork W2896073378 @default.
- W2896073378 hasRelatedWork W2950970478 @default.
- W2896073378 hasRelatedWork W3187641169 @default.
- W2896073378 hasRelatedWork W4226494652 @default.
- W2896073378 hasRelatedWork W4306768313 @default.
- W2896073378 hasVolume "8" @default.
- W2896073378 isParatext "false" @default.
- W2896073378 isRetracted "false" @default.
- W2896073378 magId "2896073378" @default.
- W2896073378 workType "article" @default.