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- W2896099071 abstract "Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing." @default.
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- W2896099071 date "2018-01-01" @default.
- W2896099071 modified "2023-09-27" @default.
- W2896099071 title "An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae" @default.
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- W2896099071 doi "https://doi.org/10.1016/j.mex.2018.10.009" @default.
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