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• W2896174911 abstract "Abstract This study was aimed to explore the differential expression of long noncoding RNAs (lnc RNA )‐ PCAT 1, miR‐145‐5p and TLR 4 in osteogenic differentiation via the Toll‐like receptor ( TLR ) signalling pathway and consequently determine the potential molecular mechanism. The mRNA s and pathways related to the osteogenic differentiation in human adipose‐derived stem cells ( hADSC s) were analysed by bioinformatics. The MiRanda and TargetScan database were employed to detect the potential binding sites of mi RNA s on lnc RNA s and mRNA s. The differential expression of lnc RNA ‐ PCAT 1, miR‐145‐5p and TLR 4 were detected by qRT ‐ PCR . Rrelated protein expression was analysed by Western blot. The targeted relationships between lnc RNA ‐ PCAT 1, miR‐145‐5p and TLR 4 were verified by dual‐luciferase reporter assay. Alkaline phosphatase ( ALP ) activity and ARS staining assays were used to measure the impacts exerted by lnc RNA PCAT 1, miR‐145‐5p and TLR 4 mRNA on osteogenic differentiation. After the induction of osteoblast differentiation, the expression of lnc RNA ‐ PCAT 1 and TLR 4 increased, while the expression of miR‐145‐5p decreased. Dual‐luciferase reporter assay confirmed the targeted relationship between lnc RNA ‐ PCAT 1, miR‐145‐5p, and TLR 4 . Lnc RNA ‐ PCAT 1 negatively regulated miR‐145‐5p and positively regulated TLR 4 . Knockdown of lnc RNA ‐ PCAT 1 or TLR 4 decreased the expression of osteogenic differentiation‐related proteins, reduced the ALP and ARS levels and the activity of the TLR signalling pathway. MiR‐145‐5p could reverse the effects of PCAT 1 and TLR 4 in hADSC s osteogenic differentiation. Lnc RNA ‐ PCAT 1 negatively regulated miR‐145‐5p, which promoted TLR 4 expression to promote osteogenic differentiation by activating the TLR signalling pathway." @default.
• W2896174911 created "2018-10-26" @default.
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• W2896174911 date "2018-10-19" @default.
• W2896174911 modified "2023-10-14" @default.
• W2896174911 title "LncRNA-PCAT1 targeting miR-145-5p promotes <i>TLR4</i> -associated osteogenic differentiation of adipose-derived stem cells" @default.
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• W2896174911 doi "https://doi.org/10.1111/jcmm.13892" @default.
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