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• W2896425032 abstract "SNARE-mediated membrane fusion is required for membrane trafficking as well as organelle biogenesis and homeostasis. The membrane fusion reaction involves sequential formation of hemifusion intermediates, whereby lipid monolayers partially mix on route to complete bilayer merger. Studies of the Saccharomyces cerevisiae lysosomal vacuole have revealed many of the fundamental mechanisms that drive the membrane fusion process, as well as features unique to organelle fusion. However, until recently, it has not been amenable to electron microscopy methods that have been invaluable for studying hemifusion in other model systems. Herein, we describe a method to visualize hemifusion intermediates during homotypic vacuole membrane fusion in vitro by transmission electron microscopy (TEM), electron tomography, and cryogenic electron microscopy (cryoEM). This method facilitates acquisition of invaluable ultrastructural data needed to comprehensively understand how fusogenic lipids and proteins contribute to SNARE-mediated membrane fusion-by-hemifusion and the unique features of organelle versus small-vesicle fusion." @default.
• W2896425032 created "2018-10-26" @default.
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• W2896425032 date "2018-10-14" @default.
• W2896425032 modified "2023-09-25" @default.
• W2896425032 title "Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy" @default.
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• W2896425032 doi "https://doi.org/10.1007/978-1-4939-8760-3_24" @default.
• W2896425032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30317518" @default.
• W2896425032 hasPublicationYear "2018" @default.
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