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- W2896447723 abstract "Abstract The large surplus of glycerol derived from the expanding biofuel industry raises economic and environmental concerns regarding disposal. In vitro synthetic biology is emerging as a useful biomanufacturing platform while the conversion of glycerol is rarely investigated. Here we develop a thermostable in vitro synthetic biosystem consisting of three enzymatic cascades for the biotransformation of glycerol into valuable chemicals with different degrees of reduction. Condensation of glycerol, phenol, and ammonium into l -tyrosine is achieved using four enzymes without the assistance of NAD + /NADH-related redox reactions. Production of chemicals with high degrees of reduction (e.g., optically pure l -lactate and d -lactate) is also verified through coupling with an NADH-regeneration system. The biotransformation of glycerol and ammonium into l -serine is achieved using four enzymes with self-sufficient NADH recycling." @default.
- W2896447723 created "2018-10-26" @default.
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- W2896447723 date "2018-10-24" @default.
- W2896447723 modified "2023-10-10" @default.
- W2896447723 title "Production of value-added chemicals from glycerol using in vitro enzymatic cascades" @default.
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- W2896447723 doi "https://doi.org/10.1038/s42004-018-0070-7" @default.
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