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- W2896449415 abstract "Objectives: In this research we assess in-vitro susceptibility of Pseudomonasaeruginosa (P. aeruginosa) using the ethanolic extract of medicinal plant Tabebuia impetiginosa(dried inner bark). To evaluate the synergistic effect of ethanolic extract of Tabebuia impetiginosacombine with ciprofloxacin In-vitro anti-Pseudomonas aeruginosa activities of the extracts andciprofloxacin were confirmed, and synergism was verified for this combine extracts. StudyDesign: Experimental study. Period: October 2016 to February 2017. Place of Experiment:Rashid Latif Medical College, Lahore. Method: Diffusion method tests are mostly qualitativemethods that are used to identify the antimicrobial activity, resistance and synergistic effect. Thefresh plants inner bark was grinded and soaked in 95% ethanol for extraction. The antibacterialsensitivity of this compound against P. aeruginosa was assessed using the diffusion method.About 1000mg of grinded menstruum was added in 800ml of petroleum ether in a conicalflask and adjust in rotary shaker at 100 rpm for 12 hours and then the final extract was filteredwith 0.45mμ filter membrane and centrifuged at 2000rpm for 15 minutes. The final extract wasredissolved in ciprofloxacin solution (10 for bioassay analysis. Results: We concludedthat the fresh ethanolic extract of Tabebuia impetiginosa with ciprofloxacin has high antibacterialpotency against P. aeruginosa which is prominent then a single. However this was not pureextract and if it is refined then it might gives significant antibacterial activity at low concentration.There is still need to test Tabebuia impetiginosa extract for antibacterial activity and to checksynergistic effect with other drugs in-vivo against Pseudomonas aeruginosa. Extract presentedthe highest synergism rate with antimicrobial drug. Conclusion: In-vitro study showed Tabebuiaimpetiginosa fresh inner bark extract with ciprofloxacin dilution have significant antibacterialactivity against Pseudomonas aeruginosa with P.value <0.001. Isolates were susceptible tothis combine solution with mean zone diameter of 16.15 ± 0.95 mm and no regrowth wasnoticed. In the present research the synergistic effect of ciprofloxacin antibiotic with Tabebuiaimpetiginosa ethanolic extract were observe against Pseudomonas aeruginosa bacteria." @default.
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- W2896449415 date "2018-10-10" @default.
- W2896449415 modified "2023-09-27" @default.
- W2896449415 title "ANTI-PSEUDOMONAS AERUGINOSA DRUG" @default.
- W2896449415 doi "https://doi.org/10.29309/tpmj/18.4509" @default.
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