FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896467673> ?p ?o ?g. }

• W2896467673 endingPage "3515" @default.
• W2896467673 startingPage "3505" @default.
• W2896467673 abstract "Primary human hepatocytes (PHHs) remain the gold standard for in vitro testing in the field of pharmacology and toxicology. One crucial parameter influencing the results of in vitro tests is the incubation period with test compounds. It has been suggested that longer incubation periods may be critical for the prediction of repeated dose toxicity. However, a study that systematically analyzes the relationship between incubation period and cytotoxicity in PHHs is not available. To close this gap, 30 compounds were tested in a concentration-dependent manner for cytotoxicity in cultivated cryopreserved PHHs (three donors per compound) for 1, 2 and 7 days. The median of the EC50 values of all compounds decreased 1.78-fold on day 2 compared to day 1, and 1.89-fold on day 7 compared to day 1. Median values of EC50 ratios of all compounds at day 2 and day 7 were close to one but for individual compounds the ratio increased up to almost six. Strong correlations were obtained for EC50 on day 1 and day 7 (R = 0.985; 95% CI 0.960-0.994), day 1 and day 2 (R = 0.964; 95% CI 0.910-0.986), as well as day 2 and day 7 (R = 0.981; 95% CI 0.955-0.992). However, compound specific differences also occurred. Whereas, for example, busulfan showed a relatively strong increase on day 7 compared to day 1, cytotoxicity of acetaminophen did not increase during longer incubation periods. To validate the observed correlations, a publicly available data set, containing data on the cytotoxicity of human hepatocytes cultivated as spheroids for incubation periods of 5 and 14 days, was analyzed. A high correlation coefficient of EC50 values at day 5 and day 14 was obtained (R = 0.894; 95% CI 0.798-0.945). In conclusion, the median cytotoxicity of the test compounds increased between 1 and 2 days of incubation, with no or only a minimal further increase until day 7. It remains to be studied whether the different results obtained for some individual compounds after longer exposure periods would correspond better to human-repeated dose toxicity." @default.
• W2896467673 created "2018-10-26" @default.
• W2896467673 creator A5015598139 @default.
• W2896467673 creator A5021780496 @default.
• W2896467673 creator A5022661690 @default.
• W2896467673 creator A5028874532 @default.
• W2896467673 creator A5032766569 @default.
• W2896467673 creator A5039644016 @default.
• W2896467673 creator A5047006269 @default.
• W2896467673 creator A5049499911 @default.
• W2896467673 creator A5058234592 @default.
• W2896467673 creator A5059609442 @default.
• W2896467673 creator A5060966054 @default.
• W2896467673 creator A5066637727 @default.
• W2896467673 creator A5068024327 @default.
• W2896467673 creator A5068941598 @default.
• W2896467673 creator A5069586805 @default.
• W2896467673 creator A5070341110 @default.
• W2896467673 creator A5075610512 @default.
• W2896467673 date "2018-10-13" @default.
• W2896467673 modified "2023-10-17" @default.
• W2896467673 title "Relevance of the incubation period in cytotoxicity testing with primary human hepatocytes" @default.
• W2896467673 cites W1787976212 @default.
• W2896467673 cites W1855404173 @default.
• W2896467673 cites W1929479056 @default.
• W2896467673 cites W1978833603 @default.
• W2896467673 cites W1991671862 @default.
• W2896467673 cites W2035257048 @default.
• W2896467673 cites W2043875880 @default.
• W2896467673 cites W2066439022 @default.
• W2896467673 cites W2074103138 @default.
• W2896467673 cites W2075455619 @default.
• W2896467673 cites W2085421312 @default.
• W2896467673 cites W2089338956 @default.
• W2896467673 cites W2120633445 @default.
• W2896467673 cites W2122345241 @default.
• W2896467673 cites W2132304265 @default.
• W2896467673 cites W2134520937 @default.
• W2896467673 cites W2150256003 @default.
• W2896467673 cites W2158248638 @default.
• W2896467673 cites W2171534546 @default.
• W2896467673 cites W2174563006 @default.
• W2896467673 cites W2256377931 @default.
• W2896467673 cites W2270994786 @default.
• W2896467673 cites W2278684016 @default.
• W2896467673 cites W2310768308 @default.
• W2896467673 cites W2312101167 @default.
• W2896467673 cites W2326651596 @default.
• W2896467673 cites W2405584860 @default.
• W2896467673 cites W2407121306 @default.
• W2896467673 cites W2465378683 @default.
• W2896467673 cites W2468744958 @default.
• W2896467673 cites W2553370301 @default.
• W2896467673 cites W2565416332 @default.
• W2896467673 cites W2625311319 @default.
• W2896467673 cites W2748747880 @default.
• W2896467673 cites W2765128648 @default.
• W2896467673 cites W4211193493 @default.
• W2896467673 doi "https://doi.org/10.1007/s00204-018-2302-0" @default.
• W2896467673 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30317417" @default.
• W2896467673 hasPublicationYear "2018" @default.
• W2896467673 type Work @default.
• W2896467673 sameAs 2896467673 @default.
• W2896467673 citedByCount "35" @default.
• W2896467673 countsByYear W28964676732018 @default.
• W2896467673 countsByYear W28964676732019 @default.
• W2896467673 countsByYear W28964676732020 @default.
• W2896467673 countsByYear W28964676732021 @default.
• W2896467673 countsByYear W28964676732022 @default.
• W2896467673 countsByYear W28964676732023 @default.
• W2896467673 crossrefType "journal-article" @default.
• W2896467673 hasAuthorship W2896467673A5015598139 @default.
• W2896467673 hasAuthorship W2896467673A5021780496 @default.
• W2896467673 hasAuthorship W2896467673A5022661690 @default.
• W2896467673 hasAuthorship W2896467673A5028874532 @default.
• W2896467673 hasAuthorship W2896467673A5032766569 @default.
• W2896467673 hasAuthorship W2896467673A5039644016 @default.
• W2896467673 hasAuthorship W2896467673A5047006269 @default.
• W2896467673 hasAuthorship W2896467673A5049499911 @default.
• W2896467673 hasAuthorship W2896467673A5058234592 @default.
• W2896467673 hasAuthorship W2896467673A5059609442 @default.
• W2896467673 hasAuthorship W2896467673A5060966054 @default.
• W2896467673 hasAuthorship W2896467673A5066637727 @default.
• W2896467673 hasAuthorship W2896467673A5068024327 @default.
• W2896467673 hasAuthorship W2896467673A5068941598 @default.
• W2896467673 hasAuthorship W2896467673A5069586805 @default.
• W2896467673 hasAuthorship W2896467673A5070341110 @default.
• W2896467673 hasAuthorship W2896467673A5075610512 @default.
• W2896467673 hasBestOaLocation W28964676732 @default.
• W2896467673 hasConcept C109316439 @default.
• W2896467673 hasConcept C126322002 @default.
• W2896467673 hasConcept C140793950 @default.
• W2896467673 hasConcept C143701427 @default.
• W2896467673 hasConcept C185592680 @default.
• W2896467673 hasConcept C202751555 @default.
• W2896467673 hasConcept C25642318 @default.
• W2896467673 hasConcept C29730261 @default.
• W2896467673 hasConcept C33070731 @default.

• next