Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896525135> ?p ?o ?g. }
- W2896525135 endingPage "e1007377" @default.
- W2896525135 startingPage "e1007377" @default.
- W2896525135 abstract "Extrachromosomal hereditary elements such as organelles, viruses, and plasmids are important for the cell fitness and survival. Their transcription is dependent on host cellular RNA polymerase (RNAP) or intrinsic RNAP encoded by these elements. The yeast Kluyveromyces lactis contains linear cytoplasmic DNA virus-like elements (VLEs, also known as linear plasmids) that bear genes encoding putative non-canonical two-subunit RNAP. Here, we describe the architecture and identify the evolutionary origin of this transcription machinery. We show that the two RNAP subunits interact in vivo, and this complex interacts with another two VLE-encoded proteins, namely the mRNA capping enzyme and a putative helicase. RNAP, mRNA capping enzyme and the helicase also interact with VLE-specific DNA in vivo. Further, we identify a promoter sequence element that causes 5' mRNA polyadenylation of VLE-specific transcripts via RNAP slippage at the transcription initiation site, and structural elements that precede the termination sites. As a result, we present a first model of the yeast virus-like element transcription initiation and intrinsic termination. Finally, we demonstrate that VLE RNAP and its promoters display high similarity to poxviral RNAP and promoters of early poxviral genes, respectively, thereby pointing to their evolutionary origin." @default.
- W2896525135 created "2018-10-26" @default.
- W2896525135 creator A5005125626 @default.
- W2896525135 creator A5034346151 @default.
- W2896525135 creator A5043733821 @default.
- W2896525135 creator A5046473165 @default.
- W2896525135 creator A5086798535 @default.
- W2896525135 creator A5091758668 @default.
- W2896525135 date "2018-10-22" @default.
- W2896525135 modified "2023-10-16" @default.
- W2896525135 title "Transcription apparatus of the yeast virus-like elements: Architecture, function, and evolutionary origin" @default.
- W2896525135 cites W1182245097 @default.
- W2896525135 cites W1483456869 @default.
- W2896525135 cites W1498563943 @default.
- W2896525135 cites W1515535764 @default.
- W2896525135 cites W1532631382 @default.
- W2896525135 cites W1591364243 @default.
- W2896525135 cites W1593142205 @default.
- W2896525135 cites W1602283030 @default.
- W2896525135 cites W1652624883 @default.
- W2896525135 cites W1671005900 @default.
- W2896525135 cites W1803990739 @default.
- W2896525135 cites W1825884898 @default.
- W2896525135 cites W1834965417 @default.
- W2896525135 cites W1964571012 @default.
- W2896525135 cites W1967826636 @default.
- W2896525135 cites W1970255065 @default.
- W2896525135 cites W1977752256 @default.
- W2896525135 cites W1977758308 @default.
- W2896525135 cites W1978139942 @default.
- W2896525135 cites W1985421947 @default.
- W2896525135 cites W1987568156 @default.
- W2896525135 cites W1987642969 @default.
- W2896525135 cites W1991366673 @default.
- W2896525135 cites W1992170617 @default.
- W2896525135 cites W1994342611 @default.
- W2896525135 cites W1996431842 @default.
- W2896525135 cites W2003583311 @default.
- W2896525135 cites W2005711148 @default.
- W2896525135 cites W2006223462 @default.
- W2896525135 cites W2012502501 @default.
- W2896525135 cites W2016293543 @default.
- W2896525135 cites W2018364304 @default.
- W2896525135 cites W2019786536 @default.
- W2896525135 cites W2020098756 @default.
- W2896525135 cites W2020392347 @default.
- W2896525135 cites W2020447874 @default.
- W2896525135 cites W2021970306 @default.
- W2896525135 cites W2023170539 @default.
- W2896525135 cites W2023450955 @default.
- W2896525135 cites W2025576332 @default.
- W2896525135 cites W2025917903 @default.
- W2896525135 cites W2026814655 @default.
- W2896525135 cites W2030080538 @default.
- W2896525135 cites W2030517878 @default.
- W2896525135 cites W2040531028 @default.
- W2896525135 cites W2047838086 @default.
- W2896525135 cites W2049286715 @default.
- W2896525135 cites W2054898430 @default.
- W2896525135 cites W2058217073 @default.
- W2896525135 cites W2066236010 @default.
- W2896525135 cites W2067911105 @default.
- W2896525135 cites W2068724508 @default.
- W2896525135 cites W2072021660 @default.
- W2896525135 cites W2075743405 @default.
- W2896525135 cites W2076561604 @default.
- W2896525135 cites W2082791493 @default.
- W2896525135 cites W2086826197 @default.
- W2896525135 cites W2088385058 @default.
- W2896525135 cites W2089709563 @default.
- W2896525135 cites W2093588155 @default.
- W2896525135 cites W2094109477 @default.
- W2896525135 cites W2095370447 @default.
- W2896525135 cites W2098571862 @default.
- W2896525135 cites W2102691305 @default.
- W2896525135 cites W2104057378 @default.
- W2896525135 cites W2105671982 @default.
- W2896525135 cites W2107798063 @default.
- W2896525135 cites W2111647009 @default.
- W2896525135 cites W2115167726 @default.
- W2896525135 cites W2121649903 @default.
- W2896525135 cites W2126656199 @default.
- W2896525135 cites W2127322768 @default.
- W2896525135 cites W2130652548 @default.
- W2896525135 cites W2134698924 @default.
- W2896525135 cites W2148151935 @default.
- W2896525135 cites W2161151688 @default.
- W2896525135 cites W2170860554 @default.
- W2896525135 cites W2199968103 @default.
- W2896525135 cites W2336330109 @default.
- W2896525135 cites W2409319157 @default.
- W2896525135 cites W2610994034 @default.
- W2896525135 cites W289557169 @default.
- W2896525135 cites W3107871404 @default.
- W2896525135 cites W311549123 @default.
- W2896525135 cites W563089146 @default.
- W2896525135 doi "https://doi.org/10.1371/journal.ppat.1007377" @default.
- W2896525135 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6211774" @default.