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- W2896526078 abstract "Defining protein-protein interactions (PPIs) is central to the biological sciences. Here, we present a novel platform - Affinity Capture of Polyribosomes followed by RNA sequencing (ACAPseq) - for identifying PPIs. ACAPseq harnesses the power of massively parallel RNA sequencing (RNAseq) to quantify the enrichment of polyribosomes based on the affinity of their associated nascent polypeptides for an immobilized protein ‘bait’. This method was developed and tested using neonatal mouse brain polyribosomes and a variety of extracellular domains as baits. Of 92 baits tested, 25 identified one or more binding partners that appear to be biologically relevant; additional candidate partners remain to be validated. ACAPseq can detect binding to targets that are present at less than 1 part in 100,000 in the starting polyribosome preparation. One of the observed PPIs was analyzed in detail, revealing the mode of homophilic binding for Protocadherin-9 (PCDH9), a non-clustered Protocadherin family member." @default.
- W2896526078 created "2018-10-26" @default.
- W2896526078 creator A5003456907 @default.
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- W2896526078 date "2018-10-22" @default.
- W2896526078 modified "2023-10-17" @default.
- W2896526078 title "Affinity capture of polyribosomes followed by RNAseq (ACAPseq), a discovery platform for protein-protein interactions" @default.
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- W2896526078 doi "https://doi.org/10.7554/elife.40982" @default.
- W2896526078 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6197854" @default.
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