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- W2896535916 abstract "In the current study we describe how we have exploited on-tissue enzyme assisted derivatisation with micro-Liquid-Extraction-for-Surface-Analysis and nano-liquid chromatography-mass spectrometry for the imaging of oxysterols in rodent brain. We find 24S-hydroxycholesterol (24S-HC) to be the most abundant oxysterol in all brain regions being at highest concentration in striatum and thalamus and at lowest levels in grey matter of cerebellum. We are also able, for the first time, to definitively identify 3β,7α-dihydroxycholest-5-en-(25R)26-oic acid in brain, where it is most abundant in cerebellum. We confirm that 24S-HC is essentially absent from brain of the CYP46A1 knockout mouse being replaced by lower levels of 25-hydroxycholesterol." @default.
- W2896535916 created "2018-10-26" @default.
- W2896535916 creator A5030641085 @default.
- W2896535916 date "2018-01-01" @default.
- W2896535916 modified "2023-09-24" @default.
- W2896535916 title "Imaging Oxysterols in Mouse Brain by On-Tissue Derivatisation-Robotic Liquid Micro-Extraction Surface Analysis-Liquid Chromatography Mass Spectrometry" @default.
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