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- W2896536905 abstract "Droplet digital PCR (ddPCR) allows precise quantitation of individual copies of DNA. Using allele-specific fluorescent oligonucleotide probes, ddPCR can enumerate both mutant and wild-type alleles enabling highly sensitive detection and quantitation of the variant allele frequency of mutated genes. In this protocol, we describe a method for using ddPCR to detect mutations in genomic DNA with a sensitivity of up to 1 in 50,000 DNA copies." @default.
- W2896536905 created "2018-10-26" @default.
- W2896536905 creator A5085769609 @default.
- W2896536905 date "2018-10-23" @default.
- W2896536905 modified "2023-09-24" @default.
- W2896536905 title "Rare Variant Quantitation Using Droplet Digital PCR" @default.
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- W2896536905 doi "https://doi.org/10.1007/978-1-4939-8876-1_18" @default.
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