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- W2896544812 abstract "The interaction of 6-hydroxyflavone (6HF) with hen egg white lysozyme (HEWL) has been executed using multi-spectroscopic and computational methods. Steady state fluorescence studies indicated that static quenching mechanism is involved in the binding of 6HF with HEWL, which was further supported by excited state lifetime and UV-vis absorption studies. The binding constant (Kb) of the HEWL-6HF complex was observed to be 6.44 ± 0.09 × 104 M-1 at 293 K, which decreases with the increase in temperature. The calculation of the thermodynamic quantities showed that the binding is exothermic in nature with a negative enthalpy change (ΔH = -11.91 ± 1.02 kJ mol-1) along with a positive entropy change (ΔS = +51.36 ± 2.43 J K-1 mol-1), and the major forces responsible for the binding are hydrogen bonding and hydrophobic interactions. The possibility of energy transfer from tryptophan (Trp) residue to the 6HF ligand was observed from Fo¨rster's theory. The inclusion of 6HF within the binding site of HEWL induces some micro-environmental changes around the Trp residues as indicated by synchronous and three-dimensional (3D) fluorescence studies. The changes in secondary structural components of HEWL are observed on binding with 6HF along with a reduction in % α-helical content. Computational studies correlate well with the experimental finding, and the ligand 6HF is found to bind near to Trp 62 and Trp 63 residues of HEWL. Altogether, the present study provides an insight into the interaction dynamics and energetics of the binding of 6HF to HEWL. Communicated by Ramaswamy H. Sarma." @default.
- W2896544812 created "2018-10-26" @default.
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- W2896544812 date "2018-12-10" @default.
- W2896544812 modified "2023-10-02" @default.
- W2896544812 title "An insight into the binding of 6-hydroxyflavone with hen egg white lysozyme: a combined approach of multi-spectroscopic and computational studies" @default.
- W2896544812 cites W1491824558 @default.
- W2896544812 cites W1824722086 @default.
- W2896544812 cites W1934853983 @default.
- W2896544812 cites W1964958542 @default.
- W2896544812 cites W1966078827 @default.
- W2896544812 cites W1973309411 @default.
- W2896544812 cites W1973387903 @default.
- W2896544812 cites W1984729902 @default.
- W2896544812 cites W1985034086 @default.
- W2896544812 cites W1986687052 @default.
- W2896544812 cites W1988090932 @default.
- W2896544812 cites W1991767480 @default.
- W2896544812 cites W1991794210 @default.
- W2896544812 cites W1995816895 @default.
- W2896544812 cites W1996112440 @default.
- W2896544812 cites W1996734538 @default.
- W2896544812 cites W1997372874 @default.
- W2896544812 cites W1997872408 @default.
- W2896544812 cites W2001345257 @default.
- W2896544812 cites W2001486675 @default.
- W2896544812 cites W2001770439 @default.
- W2896544812 cites W2002590736 @default.
- W2896544812 cites W2003547729 @default.
- W2896544812 cites W2004066943 @default.
- W2896544812 cites W2006382951 @default.
- W2896544812 cites W2013885842 @default.
- W2896544812 cites W2015698292 @default.
- W2896544812 cites W2017206337 @default.
- W2896544812 cites W2017429979 @default.
- W2896544812 cites W2020389778 @default.
- W2896544812 cites W2024857145 @default.
- W2896544812 cites W2024891605 @default.
- W2896544812 cites W2037030371 @default.
- W2896544812 cites W2038373518 @default.
- W2896544812 cites W2047644486 @default.
- W2896544812 cites W2050786084 @default.
- W2896544812 cites W2053119941 @default.
- W2896544812 cites W2055230590 @default.
- W2896544812 cites W2059091830 @default.
- W2896544812 cites W2060370206 @default.
- W2896544812 cites W2062102053 @default.
- W2896544812 cites W2063225109 @default.
- W2896544812 cites W2065329899 @default.
- W2896544812 cites W2066243047 @default.
- W2896544812 cites W2073369057 @default.
- W2896544812 cites W2075705506 @default.
- W2896544812 cites W2076148156 @default.
- W2896544812 cites W2077234232 @default.
- W2896544812 cites W2078448643 @default.
- W2896544812 cites W2081693079 @default.
- W2896544812 cites W2089506931 @default.
- W2896544812 cites W2094517427 @default.
- W2896544812 cites W2104017463 @default.
- W2896544812 cites W2114367386 @default.
- W2896544812 cites W2116060738 @default.
- W2896544812 cites W2119638146 @default.
- W2896544812 cites W2132629607 @default.
- W2896544812 cites W2135572502 @default.
- W2896544812 cites W2140260269 @default.
- W2896544812 cites W2147541184 @default.
- W2896544812 cites W2155476411 @default.
- W2896544812 cites W2159426997 @default.
- W2896544812 cites W2161081130 @default.
- W2896544812 cites W2171268876 @default.
- W2896544812 cites W2200379651 @default.
- W2896544812 cites W2307448996 @default.
- W2896544812 cites W2328826403 @default.
- W2896544812 cites W2488893896 @default.
- W2896544812 cites W2491458126 @default.
- W2896544812 cites W2552717109 @default.
- W2896544812 cites W2583621898 @default.
- W2896544812 cites W2597387889 @default.
- W2896544812 cites W2766003482 @default.
- W2896544812 cites W2768042791 @default.
- W2896544812 cites W2784122538 @default.
- W2896544812 cites W2802297495 @default.
- W2896544812 doi "https://doi.org/10.1080/07391102.2018.1535451" @default.
- W2896544812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30314416" @default.
- W2896544812 hasPublicationYear "2018" @default.
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