FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896560476> ?p ?o ?g. }

• W2896560476 endingPage "e179" @default.
• W2896560476 startingPage "e179" @default.
• W2896560476 abstract "In vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) enables the anatomical distribution of free radical species to be monitored. DNP enhances the MRI signal from nuclei such as 1H by irradiating tissue at the electron paramagnetic resonance (EPR) frequency of the free radical, before applying the MRI pulse sequence, thus increasing the image intensity where free radicals are present. Low-molecular-weight, stable nitroxyl compounds, including carbamoyl-PROXYL (CmP), are organic free radicals that possess SOD-like activity, and they have been used as probes in a variety of biophysical and biochemical experiments. The reduction rate of the radical probe depends on the tissue redox environment, which is influenced by factors such as overproduction of ROS and decreased antioxidant defences. In this study, we evaluated the feasibility of mitochondrial redox metabolic imaging, assessed by DNP-MRI with CmP as a molecular imaging probe, for detecting the metabolic change after radiation treatment to the tissue homogenate solution. Redox imaging of mitochondrial metabolism was performed with a low magnetic field using an in vivo DNP-MRI system (Keller, Japan Redox Inc.). The external magnetic field (B0) for EPR irradiation and MRI was fixed at 15 mT, and the radiofrequencies for EPR irradiation and MRI were 455 MHz and 683 kHz, respectively. DNP-MRI images of liver homogenate solution with or without radiation (10 or 20 gray) were obtained at 3, 5, 10, 15, 20, 25, 30, 35 and 40 min after starting measurement. The redox map was obtained by the slope of the enhanced DNP image intensity of each pixel from four pharmacokinetic images using a custom Excel macro program. Phantom redox experiments were conducted to examine the ability of CmP to participate in the mitochondrial redox reaction, and time-dependent changes in image contrast were assessed using liver tissue homogenate and KCN, an inhibitor of mitochondrial electron transfer chain. The DNP images clearly showed the significant enhancement in tissue homogenate solution by CmP. The enhancement of DNP-image was gradually reduced by liver homogenate solution due to reduction of CmP and the reduction rate of CmP was increased by the treatment of radiation. Interestingly, reduction of CmP in both homogenates with or without radiation were completely inhibited by KCN treatment. It is suggested that enhancement of CmP reduction is related in mitochondrial electron transfer system. We demonstrated that the mitochondrial redox metabolic change in the electron transfer chain after radiotherapy could be monitored by DNP-MRI with CmP as a redox probe." @default.
• W2896560476 created "2018-10-26" @default.
• W2896560476 creator A5021329884 @default.
• W2896560476 creator A5051022830 @default.
• W2896560476 creator A5077293109 @default.
• W2896560476 creator A5085728678 @default.
• W2896560476 creator A5091061721 @default.
• W2896560476 date "2018-11-01" @default.
• W2896560476 modified "2023-10-16" @default.
• W2896560476 title "Imaging of Mitochondria Metabolic Change after Radiation Treatment using Dynamic Nuclear Polarization (DNP)-MRI" @default.
• W2896560476 doi "https://doi.org/10.1016/j.ijrobp.2018.07.663" @default.
• W2896560476 hasPublicationYear "2018" @default.
• W2896560476 type Work @default.
• W2896560476 sameAs 2896560476 @default.
• W2896560476 citedByCount "0" @default.
• W2896560476 crossrefType "journal-article" @default.
• W2896560476 hasAuthorship W2896560476A5021329884 @default.
• W2896560476 hasAuthorship W2896560476A5051022830 @default.
• W2896560476 hasAuthorship W2896560476A5077293109 @default.
• W2896560476 hasAuthorship W2896560476A5085728678 @default.
• W2896560476 hasAuthorship W2896560476A5091061721 @default.
• W2896560476 hasConcept C111337013 @default.
• W2896560476 hasConcept C121332964 @default.
• W2896560476 hasConcept C12554922 @default.
• W2896560476 hasConcept C126838900 @default.
• W2896560476 hasConcept C139066938 @default.
• W2896560476 hasConcept C143409427 @default.
• W2896560476 hasConcept C150903083 @default.
• W2896560476 hasConcept C178790620 @default.
• W2896560476 hasConcept C185544564 @default.
• W2896560476 hasConcept C185592680 @default.
• W2896560476 hasConcept C187961010 @default.
• W2896560476 hasConcept C207001950 @default.
• W2896560476 hasConcept C46141821 @default.
• W2896560476 hasConcept C55493867 @default.
• W2896560476 hasConcept C55904794 @default.
• W2896560476 hasConcept C71924100 @default.
• W2896560476 hasConcept C86803240 @default.
• W2896560476 hasConceptScore W2896560476C111337013 @default.
• W2896560476 hasConceptScore W2896560476C121332964 @default.
• W2896560476 hasConceptScore W2896560476C12554922 @default.
• W2896560476 hasConceptScore W2896560476C126838900 @default.
• W2896560476 hasConceptScore W2896560476C139066938 @default.
• W2896560476 hasConceptScore W2896560476C143409427 @default.
• W2896560476 hasConceptScore W2896560476C150903083 @default.
• W2896560476 hasConceptScore W2896560476C178790620 @default.
• W2896560476 hasConceptScore W2896560476C185544564 @default.
• W2896560476 hasConceptScore W2896560476C185592680 @default.
• W2896560476 hasConceptScore W2896560476C187961010 @default.
• W2896560476 hasConceptScore W2896560476C207001950 @default.
• W2896560476 hasConceptScore W2896560476C46141821 @default.
• W2896560476 hasConceptScore W2896560476C55493867 @default.
• W2896560476 hasConceptScore W2896560476C55904794 @default.
• W2896560476 hasConceptScore W2896560476C71924100 @default.
• W2896560476 hasConceptScore W2896560476C86803240 @default.
• W2896560476 hasIssue "3" @default.
• W2896560476 hasLocation W28965604761 @default.
• W2896560476 hasOpenAccess W2896560476 @default.
• W2896560476 hasPrimaryLocation W28965604761 @default.
• W2896560476 hasRelatedWork W1971207319 @default.
• W2896560476 hasRelatedWork W1972375624 @default.
• W2896560476 hasRelatedWork W1973615240 @default.
• W2896560476 hasRelatedWork W1991237059 @default.
• W2896560476 hasRelatedWork W2028678486 @default.
• W2896560476 hasRelatedWork W2093932502 @default.
• W2896560476 hasRelatedWork W2212793539 @default.
• W2896560476 hasRelatedWork W2322334504 @default.
• W2896560476 hasRelatedWork W2374685867 @default.
• W2896560476 hasRelatedWork W4293071741 @default.
• W2896560476 hasVolume "102" @default.
• W2896560476 isParatext "false" @default.
• W2896560476 isRetracted "false" @default.
• W2896560476 magId "2896560476" @default.
• W2896560476 workType "article" @default.